Inbred corn line II15

ABSTRACT

Inbred corn line, designated II15, is disclosed. The invention relates to the seeds of inbred corn line II15, to the plants and plant parts of inbred corn line II15, and to methods for producing a corn plant, either inbred or hybrid, by crossing inbred corn line II15 with itself or another corn line. The invention also relates to products produced from the seeds, plants, or parts thereof, of inbred corn line II15 and/or of the hybrids produced using the inbred as a parent. The invention further relates to methods for producing a corn plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other inbred corn lines derived from inbred corn line II15.

CROSS-REFERENCE TO RELATED PATENT APPLICATIONS

The present application claims priority to, and the benefit of U.S.Provisional Patent Application Ser. No. 61/954,741, filed on Mar. 18,2014, which is herein incorporated by reference in its entirety.

FIELD OF THE INVENTION

The present invention relates to new and distinctive corn inbred lines(Zea mays, also known as maize), designated BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65.

BACKGROUND OF THE INVENTION

The disclosures, including the claims, figures and/or drawings, of eachand every patent, patent application, and publication cited herein arehereby incorporated herein by reference in their entireties.

The following description includes information that may be useful inunderstanding the present invention. It is not an admission that any ofthe information provided herein is prior art or relevant to thepresently claimed inventions, or that any publication specifically orimplicitly referenced is prior art.

There are numerous steps in the development of any novel, desirableplant germplasm. Plant breeding begins with the analysis and definitionof problems and weaknesses of the current germplasm, the establishmentof program goals, and the definition of specific breeding objectives.The next step is selection of germplasm that possess the traits to meetthe program goals. The goal is to combine in a single variety or hybridan improved combination of desirable traits from the parental germplasm.These important traits may include higher yield, resistance to diseasesand insects, better stalks and roots, tolerance to drought and heat,reduction of grain moisture at harvest as well as better agronomicquality. With mechanical harvesting of many crops, uniformity of plantcharacteristics such as germination and stand establishment, growthrate, maturity and plant and ear height is important.

Choice of breeding or selection methods depends on the mode of plantreproduction, the heritability of the trait(s) being improved, and thetype of cultivar used commercially (e.g., F₁ hybrid cultivar, purelinecultivar, etc.). For highly heritable traits, a choice of superiorindividual plants evaluated at a single location will be effective,whereas for traits with low heritability, selection should be based onmean values obtained from replicated evaluations of families of relatedplants. Popular selection methods commonly include pedigree selection,modified pedigree selection, mass selection, recurrent selection, andbackcross breeding.

The complexity of inheritance influences choice of breeding method.Backcross breeding is used to transfer one or a few favorable genes fora heritable trait into a desirable cultivar. This approach has been usedextensively for breeding disease-resistant cultivars; nevertheless, itis also suitable for the adjustment and selection of morphologicalcharacters, color characteristics and simply inherited quantitativecharacters such as earliness, plant height or seed size and shape.Various recurrent selection techniques are used to improvequantitatively inherited traits controlled by numerous genes. The use ofrecurrent selection in self-pollinating crops depends on the ease ofpollination, the frequency of successful hybrids from each pollination,and the number of hybrid offspring from each successful cross.

Each breeding program should include a periodic, objective evaluation ofthe efficiency of the breeding procedure. Evaluation criteria varydepending on the goal and objectives, but should include gain fromselection per year based on comparisons to an appropriate standard,overall value of the advanced breeding lines, and number of successfulcultivars produced per unit of input (e.g., per year, per dollarexpended, etc.).

Promising advanced breeding lines are thoroughly tested per se and inhybrid combination and compared to appropriate standards in environmentsrepresentative of the commercial target area(s) for three or more years.The best lines are candidates for use as parents in new commercialcultivars; those still deficient in a few traits may be used as parentsto produce new populations for further selection.

These processes, which lead to the final step of marketing anddistribution, usually take from eight to twelve years from the time thefirst cross is made. Therefore, development of new cultivars is atime-consuming process that requires precise forward planning, efficientuse of resources, and a focus on clear objectives.

A most difficult task is the identification of individuals that aregenetically superior, because for most traits the true genotypic valueis masked by other confounding plant traits or environmental factors.One method of identifying a superior plant is to observe its performancerelative to other experimental plants and to a widely grown standardcultivar. If a single observation is inconclusive, replicatedobservations provide a better estimate of its genetic worth.

The goal of corn breeding is to develop new, unique and superior corninbred lines and hybrids. The breeder initially selects and crosses twoor more parental lines, followed by repeated self pollination or selfingand selection, producing many new genetic combinations. Another methodused to develop new, unique and superior corn inbred lines and hybridsoccurs when the breeder selects and crosses two or more parental lines,followed by haploid induction and chromosome doubling that results inthe development of dihaploid inbred lines. The breeder can theoreticallygenerate billions of different genetic combinations via crossing,selfing and mutations and the same is true for the utilization of thedihaploid breeding method.

Each year, the plant breeder selects the germplasm to advance to thenext generation. This germplasm is grown under unique and differentgeographical, climatic and soil conditions, and further selections arethen made, during and at the end of the growing season. The inbred lineswhich are developed are unpredictable. This unpredictability is becausethe breeder's selection occurs in unique environments, with no controlat the DNA level (using conventional breeding procedures or dihaploidbreeding procedures), and with millions of different possible geneticcombinations being generated. A breeder of ordinary skill in the artcannot predict the final resulting lines he develops, except possibly ina very gross and general fashion. This unpredictability results in theexpenditure of large research funds to develop several superior new corninbred lines.

The development of commercial corn hybrids requires the development ofhomozygous inbred lines, the crossing of these lines, and the evaluationof the crosses. Pedigree breeding and recurrent selection breedingmethods are used to develop inbred lines from breeding populations.Breeding programs combine desirable traits from two or more inbred linesor various broad-based sources into breeding pools from which inbredlines are developed by selfing and selection of desired phenotypes orthrough the dihaploid breeding method followed by the selection ofdesired phenotypes. The new inbreds are crossed with other inbred linesand the hybrids from these crosses are evaluated to determine which havecommercial potential.

Pedigree breeding is used commonly for the improvement ofself-pollinating crops or inbred lines of cross-pollinating crops. Twoparents which possess favorable, complementary traits are crossed toproduce an F₁. An F₂ population is produced by selfing one or severalF₁s or by intercrossing two F₁s (sib mating). Selection of the bestindividuals is usually begun in the F₂ population; then, beginning inthe F₃, the best individuals in the best families are selected.Replicated testing of families, or hybrid combinations involvingindividuals of these families, often follows in the F₄ generation toimprove the effectiveness of selection for traits with low heritability.At an advanced stage of inbreeding (i.e., F₆ and F₇), the best lines ormixtures of phenotypically similar lines are tested for potentialrelease as new cultivars. Similarly, the development of new inbred linesthrough the dihaploid system requires the selection of the best inbredsfollowed by four to five years of testing in hybrid combinations inreplicated plots.

Mass and recurrent selections can be used to improve populations ofeither self- or cross-pollinating crops. A genetically variablepopulation of heterozygous individuals is either identified or createdby intercrossing several different parents. The best plants are selectedbased on individual superiority, outstanding progeny, or excellentcombining ability. The selected plants are intercrossed to produce a newpopulation in which further cycles of selection are continued.

Backcross breeding has been used to transfer genes for a simplyinherited, highly heritable trait into a desirable cultivar or inbredline which is the recurrent parent. The source of the trait to betransferred is called the donor parent. The resulting plant is expectedto have the attributes of the recurrent parent (e.g., cultivar) and thedesirable trait transferred from the donor parent. After the initialcross, individuals possessing the phenotype of the donor parent areselected and repeatedly crossed (backcrossed) to the recurrent parent.The resulting plant is expected to have the attributes of the recurrentparent (e.g., cultivar) and the desirable trait transferred from thedonor parent.

Descriptions of other breeding methods that are commonly used fordifferent traits and crops can be found in one of several referencebooks (e.g., R. W. Allard, 1960, Principles of Plant Breeding, JohnWiley and Son; Briggs, F. N. and Knowles, P. F. 1967. Introduction toPlant Breeding. Reinhold Publishing Corporation; N. W Simmonds, 1979,Principles of Crop Improvement, Longman Group Limited; W. R. Fehr, 1987,Principles of Crop Development, Macmillan Publishing Co.; N. F. Jensen,1988, Plant Breeding Methodology, John Wiley & Sons).

Proper testing should detect any major faults and establish the level ofsuperiority or improvement over current cultivars. In addition toshowing superior performance, there must be a demand for a new cultivarthat is compatible with industry standards or which creates a newmarket. The introduction of a new cultivar will incur additional coststo the seed producer, the grower, processor and consumer for specialadvertising and marketing, altered seed and commercial productionpractices, and new product utilization. The testing preceding release ofa new cultivar should take into consideration research and developmentcosts as well as technical superiority of the final cultivar. Forseed-propagated cultivars, it must be feasible to produce seed easilyand economically.

Once the inbreds that give the best hybrid performance have beenidentified, the hybrid seed can be reproduced indefinitely as long asthe homogeneity of the inbred parent is maintained. A single-crosshybrid is produced when two inbred lines are crossed to produce the F₁progeny. A double-cross hybrid is produced from four inbred linescrossed in pairs (A×B and C×D) and then the two F₁ hybrids are crossedagain (A×B)×(C×D). Much of the hybrid vigor exhibited by F₁ hybrids islost in the next generation (F₂). Consequently, seed from hybridvarieties is not used for planting stock.

Hybrid corn seed is typically produced by a male sterility system or byincorporating manual or mechanical detasseling. Alternate strips of twocorn inbreds are planted in a field, and the pollen-bearing tassels areremoved from one of the inbreds (female). Providing that there issufficient isolation from sources of foreign corn pollen, the ears ofthe detasseled inbred will be fertilized only from the other inbred(male), and the resulting seed is therefore hybrid and will form hybridplants.

The laborious, and occasionally unreliable, detasseling process can beavoided by using cytoplasmic male-sterile (CMS) inbreds. Plants of a CMSinbred are male sterile as a result of factors resulting from thecytoplasmic, as opposed to the nuclear, genome. Thus, thischaracteristic is inherited exclusively through the female parent incorn plants, since only the female provides cytoplasm to the fertilizedseed. CMS plants are fertilized with pollen from another inbred that isnot male-sterile. Pollen from the second inbred may or may notcontribute genes that make the hybrid plants male-fertile. Seed fromdetasseled fertile corn and CMS produced seed of the same hybrid can beblended to insure that adequate pollen loads are available forfertilization when the hybrid plants are grown.

There are several methods of conferring genetic male sterilityavailable, such as multiple mutant genes at separate locations withinthe genome that confer male sterility, as disclosed in U.S. Pat. Nos.4,654,465 and 4,727,219 to Brar et al. These and all patents referred toare incorporated by reference. In addition to these methods, Albertsenet al., U.S. Pat. No. 5,432,068, have developed a system of nuclear malesterility which includes: identifying a gene which is critical to malefertility, silencing this native gene which is critical to malefertility; removing the native promoter from the essential malefertility gene and replacing it with an inducible promoter; insertingthis genetically engineered gene back into the plant; and thus creatinga plant that is male sterile because the inducible promoter is not “on”resulting in the male fertility gene not being transcribed. Fertility isrestored by inducing, or turning “on,” the promoter, which in turnallows the gene that confers male fertility to be transcribed.

There are many other methods of conferring genetic male sterility in theart, each with its own benefits and drawbacks. These methods use avariety of approaches such as delivering into the plant a gene encodinga cytotoxic substance associated with a male tissue specific promoter oran anti-sense system in which a gene critical to fertility is identifiedand an antisense to that gene is inserted in the plant (see,Fabinjanski, et al. EPO 89/0301053.8 publication number 329,308 and PCTapplication PCT/CA90/00037 published as WO 90/08828).

Another version useful in controlling male sterility makes use ofgametocides. Gametocides are not a genetic system, but rather a topicalapplication of chemicals. These chemicals affect cells that are criticalto male fertility. The application of these chemicals affects fertilityin the plants only for the growing season in which the gametocide isapplied (see Carlson, G. R., U.S. Pat. No. 4,936,904). Application ofthe gametocide, timing of the application, and genotype often limit theusefulness of the approach.

Corn is an important and valuable field crop. Thus, a continuing goal ofplant breeders is to develop stable, high yielding corn hybrids that areagronomically sound. The reasons for this goal are obviously to maximizethe amount of ears or kernels produced on the land used and to supplyfood for both humans and animals. To accomplish this goal, the cornbreeder must select and develop corn plants that have the traits thatresult in superior parental lines for producing hybrids.

The foregoing examples of the related art and limitations relatedtherewith are intended to be illustrative and not exclusive. Otherlimitations of the related art will become apparent to those of skill inthe art upon a reading of the specification.

SUMMARY OF THE INVENTION

The following embodiments and aspects thereof are described inconjunction with systems, tools and methods which are meant to beexemplary and illustrative, not limiting in scope. In variousembodiments, one or more of the above-described problems have beenreduced or eliminated, while other embodiments are directed to otherimprovements.

According to the invention, there are provided inbred corn linesdesignated BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39,II15, II17, IM5, LK1, and MM65. This invention thus relates to the seedsof inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147, CB21,CB34, CB39, II15, II17, IM5, LK1, and MM65, to the plants or partsthereof of inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, to plants or partsthereof having all the physiological and morphological characteristicsof inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21,CB34, CB39, II15, II17, IM5, LK1, and MM65 and to plants or partsthereof having all the physiological and morphological characteristicsof inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21,CB34, CB39, II15, II17, IM5, LK1, and MM65 listed in Table 1, includingbut not limited to as determined at the 5% significance level when grownin the same environmental conditions.

The invention also relates to variants, mutants and trivialmodifications of the seed or plant of inbred corn lines BB208, BB209,BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, andMM65. Parts of the inbred corn plant of the present invention are alsoprovided, such as e.g., pollen obtained from an inbred plant and anovule of the inbred plant. Variants, mutants and trivial modificationsof the seed or plant of the corn line of the present invention can begenerated by methods available to one skilled in the art, including butnot limited to, mutagenesis (e.g., chemical mutagenesis, radiationmutagenesis, transposon mutagenesis, insertional mutagenesis, signaturetagged mutagenesis, site-directed mutagenesis, and natural mutagenesis),knock-outs/knock-ins, antisense and RNA interference. For moreinformation of mutagenesis in plants, such as agents, protocols, seeAcquaah et al. (Principles of plant genetics and breeding,Wiley-Blackwell, 2007, ISBN 1405136464, 9781405136464, which is hereinincorporated by reference in its entity).

The invention also relates to a mutagenized population of inbred cornlines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15,II17, IM5, LK1, and MM65, and methods of using such populations. In someembodiments, the mutagenized population can be used in screening for newcorn lines which comprises one or more or all of the morphological andphysiological characteristics of inbred corn line BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65.In some embodiments, the new corn lines obtained from the screeningprocess comprise all of the morphological and physiologicalcharacteristics of inbred corn line BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, and one or moreadditional or different morphological and physiological characteristicsthat the inbred corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 does not have.

The mutagenized population of the present invention can be used inTargeting Induced Local Lesions in Genomes (TILLING) screening method,which combines a standard and efficient technique of mutagenesis with achemical mutagen (e.g., Ethyl methanesulfonate (EMS)) with a sensitiveDNA screening-technique that identifies single base mutations (alsocalled point mutations) in a target gene. Detailed description onmethods and compositions on TILLING® can be found in Till et al.(Discovery of induced point mutations in maize genes by TILLING, BMCPlant Biology 2004, 4:12), Weil et al., (TILLING in Grass Species, PlantPhysiology January 2009 vol. 149 no. 1 158-164), Comai, L. and S.Henikoff (“TILLING: practical single-nucleotide mutation discovery.”Plant J 45(4): 684-94), McCallum et al., (Nature Biotechnology, 18:455-457, 2000), McCallum et al., (Plant Physiology, 123: 439-442, 2000),Colbert et al., (Plant Physiol. 126(2): 480-484, 2001), U.S. Pat. No.5,994,075, U.S. Patent Application Publication No. 2004/0053236A1, andInternational Patent Application Publication Nos. WO 2005/055704 and WO2005/048692, each of which is hereby incorporated by reference for allpurposes.

The plants and seeds of the present invention include those that may beof an essentially derived variety as defined in section 41(3) of thePlant Variety Protection Act, i.e., a variety that:

-   -   1. is predominantly derived from inbred corn lines BB208, BB209,        BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5,        LK1, and MM65, or from a variety that is predominantly derived        from inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147,        CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, while        retaining the expression of the essential characteristics that        result from the genotype or combination of genotypes of inbred        corn lines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34,        CB39, II15, II17, IM5, LK1, and MM65;    -   2. is clearly distinguishable from inbred corn line BB208,        BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17,        IM5, LK1, and MM65; and    -   3. except for differences that result from the act of        derivation, conforms to the initial variety in the expression of        the essential characteristics that result from the genotype or        combination of genotypes of the initial variety.

In another aspect, the present invention provides regenerable cells foruse in tissue culture of inbred corn plant corn line BB208, BB209,BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, andMM65. The tissue culture will preferably be capable of regeneratingplants having all the physiological and morphological characteristics ofthe foregoing inbred corn plant. Preferably, the cells of such tissuecultures will be embryos, ovules, meristematic cells, seeds, callus,pollen, leaves, anthers, roots, root tips, silk, flowers, kernels, ears,cobs, husks, stalks or the like. Protoplasts produced from such tissueculture are also included in the present invention. The corn shoots,roots and whole plants regenerated from the tissue cultures are alsopart of the invention.

Also included in this invention are methods for producing a corn plantproduced by crossing the inbred corn line BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 withitself or another corn line. When crossed with itself, i.e., crossedwith another inbred corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 plant orself-pollinated, the inbred line corn line BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 will beconserved (e.g., as an inbred). When crossed with another, differentcorn line, an F₁ hybrid seed is produced. F₁ hybrid seeds and plantsproduced by growing said hybrid seeds are included in the presentinvention. A method for producing an F₁ hybrid corn seed comprisingcrossing inbred corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 corn plant with adifferent corn plant and harvesting the resultant hybrid corn seed arealso part of the invention. The hybrid corn seed produced by the methodcomprising crossing inbred corn line BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 corn plant witha different corn plant and harvesting the resultant hybrid corn seed areincluded in the invention, as are included the hybrid corn plant orparts thereof, seeds included, produced by growing said hybrid cornseed.

In another embodiment, this invention relates to a method for producingthe inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21,CB34, CB39, II15, II17, IM5, LK1, and MM65 from a collection of seeds,the collection containing both inbred corn line BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65seeds and hybrid seeds having inbred corn line BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 asa parental line. Such a collection of seeds might be a commercial bag ofseeds. Said method comprises planting the collection of seeds. Whenplanted, the collection of seeds will produce inbred corn line BB208,BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5,LK1, and MM65 plants from inbred corn line BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 seeds andhybrid plants from hybrid seeds. The plants having all the physiologicaland morphological characteristics of corn inbred BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 orhaving a decreased vigor compared to the other plants grown from thecollection of seeds are identified as inbred corn line BB208, BB209,BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, andMM65 parent plant. Said decreased vigor is due to the inbreedingdepression effect and can be identified for example by a less vigorousappearance for vegetative and/or reproductive characteristics includingshorter plant height, small ear size, ear and kernel shape, ear color orother characteristics. As previously mentioned, if the inbred corn lineBB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17,IM5, LK1, and MM65 is self-pollinated, the inbred corn line BB208,BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5,LK1, and MM65 will be preserved, therefore, the next step is controllingpollination of the inbred parent plants in a manner which preserves thehomozygosity of said inbred corn line BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 parent plant andthe final step is to harvest the resultant seed.

This invention also relates to methods for producing other inbred cornlines derived from inbred corn line BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 and to theinbred corn lines derived by the use of those methods.

In another aspect, the present invention provides transformed inbredcorn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39,II15, II17, IM5, LK1, and MM65 or parts thereof that have beentransformed so that its genetic material contains one or moretransgenes, preferably operably linked to one or more regulatoryelements. Also, the invention provides methods for producing a cornplant containing in its genetic material one or more transgenes,preferably operably linked to one or more regulatory elements, bycrossing transformed inbred corn line BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 with either asecond plant of another corn line, or a non-transformed corn plant ofthe inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21,CB34, CB39, II15, II17, IM5, LK1, and MM65, so that the genetic materialof the progeny that results from the cross contains the transgene(s),preferably operably linked to one or more regulatory elements. Theinvention also provides methods for producing a corn plant that containsin its genetic material one or more transgene(s), wherein the methodcomprises crossing the inbred corn line BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 with asecond plant of another corn line which contains one or moretransgene(s) operably linked to one or more regulatory element(s) sothat the genetic material of the progeny that results from the crosscontains the transgene(s) operably linked to one or more regulatoryelement(s). Transgenic corn plants, or parts thereof produced by themethod are in the scope of the present invention.

More specifically, the invention comprises methods for producing cornplants or seeds with at least one trait selected from the groupconsisting of male sterile, male fertile, herbicide resistant, insectresistant, disease resistant, water stress tolerant corn plants orseeds, or corn plants or seeds with modified, in particular decreased,phytate content, with modified waxy and/or amylose starch content, withmodified protein content, with modified oil content or profile, withincreased digestibility or with increased nutritional quality. Saidmethods comprise transforming the inbred corn lines BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65corn plant with nucleic acid molecules that confer, for example, malesterility, male fertility, herbicide resistance, insect resistance,disease resistance, water stress tolerance, or that can modify thephytate, the waxy and/or amylose starches, the protein or the oilcontents, the digestibility or the nutritional qualities, respectively.The transformed corn plants or seeds obtained from the provided methods,including, for example, those corn plants or seeds with male sterility,male fertility, herbicide resistance, insect resistance, diseaseresistance, water stress tolerance, modified phytate, waxy and/oramylose starches, protein or oil contents, increased digestibility andincreased nutritional quality are included in the present invention.Plants may display one or more of the above listed traits. For thepresent invention and the skilled artisan, disease is understood to befungal disease, viral disease, bacterial disease or other plantpathogenic diseases and disease resistant plant encompasses plantsresistant to fungal, viral, bacterial and other plant pathogens.

Also included in the invention are methods for producing a corn plant orseed containing in its genetic material one or more transgenes involvedwith fatty acid metabolism, carbohydrate metabolism, and starch contentsuch as waxy starch or increased amylose starch. The transgenic cornplants or seeds produced by these methods are also part of theinvention.

In another aspect, the present invention provides for methods ofintroducing one or more desired trait(s) into the inbred corn linesBB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17,IM5, LK1, and MM65 and plants or seeds obtained from such methods. Thedesired trait(s) may be, but not exclusively, a single gene, preferablya dominant but also a recessive allele. Preferably, the transferred geneor genes will confer such traits as male sterility, herbicideresistance, insect resistance, resistance for bacterial, fungal, orviral disease, male fertility, water stress tolerance, enhancednutritional quality, modified waxy content, modified amylose content,modified protein content, modified oil content, enhanced plant quality,enhanced digestibility and industrial usage. The gene or genes may benaturally occurring maize gene(s) or transgene(s) introduced throughgenetic engineering techniques. The method for introducing the desiredtrait(s) is preferably a backcrossing process making use of a series ofbackcrosses to the inbred corn lines BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 during which thedesired trait(s) is maintained by selection.

When using a transgene, the trait is generally not incorporated intoeach newly developed line such as inbred corn line BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 bydirect transformation. Rather, the more typical method used by breedersof ordinary skill in the art to incorporate the transgene is to take aline already carrying the transgene and to use such line as a donor lineto transfer the transgene into the newly developed line. The same wouldapply for a naturally occurring trait (e.g., a native trait, such as butnot limited to drought tolerance or improved nitrogen utilization) orone arising from spontaneous or induced mutations. The backcrossbreeding process comprises the following steps: (a) crossing inbred cornline BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15,II17, IM5, LK1, and MM65 plants with plants of another line thatcomprise the desired trait(s), (b) selecting the F₁ progeny plants thathave the desired trait(s); (c) crossing the selected F₁ progeny plantswith the inbred corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 plants to producebackcross progeny plants; (d) selecting for backcross progeny plantsthat have the desired trait(s) and physiological and morphologicalcharacteristics of corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 to produce selectedbackcross progeny plants; and (e) repeating steps (c) and (d) one, two,three, four, five, six, seven, eight, nine or more times in successionto produce selected, second, third, fourth, fifth, sixth, seventh,eighth, ninth or higher backcross progeny plants that comprise thedesired trait(s) and all the physiological and morphologicalcharacteristics of corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 as listed in Table 1Ato 1S, including but not limited to at a 5% significance level whengrown in the same environmental conditions. The corn plants or seedsproduced by the methods are also part of the invention. Backcrossingbreeding methods, well known to one skilled in the art of plant breedingwill be further developed in subsequent parts of the specification.

In another aspect of the invention, inbred corn lines BB208, BB209,BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, andMM65 may be used as a parent, or a single gene conversion or atransgenic inbred corn line of BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, such as BB208, BB209,BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1,MM65, and MN3/MON89034 may be used as a parent and may be crossed withanother corn line. Preferably, the single gene conversions or transgenicinbred lines will confer such traits, herbicide resistance, insectresistance, resistance for bacterial, fungal, or viral disease, malefertility, water stress tolerance, enhanced nutritional quality,modified waxy content, modified amylose content, modified proteincontent, modified oil content, enhanced plant quality, enhanceddigestibility and industrial usage. The gene or genes may be naturallyoccurring maize gene(s) (e.g., native traits) or transgene(s) introducedthrough genetic engineering techniques. The hybrid corn plants or seedshaving inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21,CB34, CB39, II15, II17, IM5, LK1, and MM65 or a single gene conversionor a transgenic inbred corn line BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 as a parentalline and having another, different, corn line as a second parental lineas discussed above are comprised in the present invention.

Any DNA sequence(s), whether from a different species or from the samespecies that is inserted into the genome using transformation isreferred to herein collectively as “transgenes.” In some embodiments ofthe invention, a transformed variant of BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 maycontain at least one transgene but could contain at least 1, 2, 3, 4, 5,6, 7, 8, 9, or 10 transgenes. In another embodiment of the invention, atransformed variant of another corn line used as the other parental linemay contain at least one transgene but could contain at least 1, 2, 3,4, 5, 6, 7, 8, 9, or 10 transgenes, such as LH287, MON810, and MON89034.

In an embodiment of this invention is a method of making a backcrossconversion of inbred corn lines BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, comprising thesteps of crossing a plant of corn inbred BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 with adonor plant comprising a mutant gene or transgene conferring a desiredtrait, selecting an F₁ progeny plant comprising the mutant gene ortransgene conferring the desired trait, and backcrossing the selected F₁progeny plant to a plant of inbred corn line BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65. Thismethod may further comprise the step of obtaining a molecular markerprofile of inbred corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 and using the molecularmarker profile to select for a progeny plant with the desired trait andthe molecular marker profile of inbred corn line BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65.In the same manner, this method may be used to produce an F₁ hybrid seedby adding a final step of crossing the desired trait conversion ofinbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34,CB39, II15, II17, IM5, LK1, and MM65 with a different corn plant to makeF₁ hybrid corn seed comprising a mutant gene or transgene conferring thedesired trait.

In some embodiments of the invention, the number of loci that may bebackcrossed into inbred corn line BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 is at least 1,2, 3, 4, or 5. A single locus may contain several transgenes, such as atransgene for disease resistance that, in the same expression vector,also contains a transgene for herbicide resistance. The gene forherbicide resistance may be used as a selectable marker and/or as aphenotypic trait. A single locus conversion of site specific integrationsystem allows for the integration of multiple genes at the convertedlocus.

In a preferred embodiment, the present invention provides methods forincreasing and producing inbred corn lines BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 seed,whether by crossing a first inbred parent corn plant with a secondinbred parent corn plant and harvesting the resultant corn seed, whereinboth said first and second inbred corn plant are the inbred corn linesBB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17,IM5, LK1, and MM65 or by planting an inbred corn seed of the inbred cornlines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15,II17, IM5, LK1, and MM65, growing an inbred corn lines BB208, BB209,BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, andMM65 plant from said seed, controlling a self pollination of the plantwhere the pollen produced by the grown inbred corn lines BB208, BB209,BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, andMM65 plant pollinates the ovules produced by the very same inbred cornlines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15,II17, IM5, LK1, and MM65 grown plant and harvesting the resultant seed.

The invention further provides methods for developing corn plants in acorn plant breeding program using plant breeding techniques includingrecurrent selection, backcrossing, pedigree breeding, molecular marker(Isozyme Electrophoresis, Restriction Fragment Length Polymorphisms(RFLPs), Randomly Amplified Polymorphic DNAs (RAPDs), Arbitrarily PrimedPolymerase Chain Reaction (AP-PCR), DNA Amplification Fingerprinting(DAF), Sequence Characterized Amplified Regions (SCARs). AmplifiedFragment Length Polymorphisms (AFLPs), and Simple Sequence Repeats(SSRs) which are also referred to as Microsatellites, etc.) enhancedselection, genetic marker enhanced selection and transformation. Cornseeds, plants, and parts thereof produced by such breeding methods arealso part of the invention.

In addition, any and all products made using the corn seeds, plants andparts thereof obtained from inbred corn lines BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 orfrom any corn line produced using inbred corn lines BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 asa direct or indirect parent are also part of the invention. Examples ofsuch corn products include but are not limited to corn meal, corn flour,corn starch, corn syrup, corn sweetener and corn oil. The origin of thecorn used in such corn products can be determined by tracking the sourceof the corn used to make the products and/or by using protein (isozyme,ELISA, etc.) and/or DNA (RFLP, PCR, SSR, SNP, EST, etc.) testing.

In addition to the exemplary aspects and embodiments described above,further aspects and embodiments will become apparent by study of thefollowing descriptions.

Definitions

In the description and tables which follow, a number of terms are used.In order to provide a clear and consistent understanding of thespecification and claims, including the scope to be given such terms,the following definitions are provided:

Allele. The allele is any of one or more alternative forms of a gene,all of which relate to one trait or characteristic. In a diploid cell ororganism, the two alleles of a given gene occupy corresponding loci on apair of homologous chromosomes.

Backcrossing. Backcrossing is a process in which a breeder repeatedlycrosses hybrid progeny back to one of the parents, for example, a firstgeneration hybrid F₁ with one of the parental genotype of the F₁ hybrid.

BT1-1, BT1. BT1-1 refers to MON810, also known as MON810Bt or BT1, isthe designation given by the Monsanto Company (St. Louis, Mo.) for thetransgenic event that, when expressed in maize, produces an endotoxinthat is efficacious against the European corn borer, Ostrinia nubilalisand certain other Lepidopteran larvae.

Collection of seeds. In the context of the present invention acollection of seeds will be a grouping of seeds mainly containingsimilar kind of seeds, for example hybrid seeds having the inbred lineof the invention as a parental line, but that may also contain, mixedtogether with this first kind of seeds, a second, different kind ofseeds, of one of the inbred parent lines, for example the inbred line ofthe present invention. A commercial bag of hybrid seeds having theinbred line of the invention as a parental line and containing also theinbred line seeds of the invention would be, for example such acollection of seeds.

CL. CL, also known as Clearfield is commercial denomination given to thegene that, when present in maize, allows the use of imidazolinoneherbicides as a weed control agent for both grasses (i.e.,monocotyledons) and broadleaves (i.e., dicotyledons).

Daily heat unit value. The daily heat unit value (also referred to asgrowing degree unit, or GDU) is calculated as follows: (the maximumdaily temperature+the minimum daily temperature)/2 minus 50. Alltemperatures are in degrees Fahrenheit. The maximum temperaturethreshold is 86 degrees, if temperatures exceed this, 86 is used. Theminimum temperature threshold is 50 degrees, if temperatures go belowthis, 50 is used. For each hybrid, it takes a certain number of GDUs toreach various stages of plant development. GDUs are a way of measuringplant maturity. GDUs can also relate to stages of growth for an inbredline.

Decreased vigor. A plant having a decreased vigor in the presentinvention is a plant that, compared to other plants has a less vigorousappearance for vegetative and/or reproductive characteristics includingshorter plant height, small ear size, ear and kernel shape, ear color orother characteristics.

Dropped ears. This is a measure of the number of dropped ears per plot,and represents the percentage of plants that dropped an ear prior toharvest.

Dry down. This is the rate at which a hybrid will reach acceptableharvest moisture.

Ear height. The ear height is a measure from the ground to the upper earnode attachment, and is measured in centimeters.

Essentially all of the physiological and morphological characteristics.A plant having essentially all of the physiological and morphologicalcharacteristics means a plant having the physiological and morphologicalcharacteristics of the recurrent parent, except for the characteristicsderived from the converted gene.

GDU pollen. The number of heat units from planting until 50% of theplants in the hybrid are shedding pollen.

GDU silk. The GDU silk (=heat unit silk) is the number of growing degreeunits (GDU) or heat units required for an inbred line or hybrid to reachsilk emergence from the time of planting.

Harvest aspect. This is a visual rating given the day of harvest or theprevious day. Hybrids are rated 1 (poorest) to 9 (best) with poorerscores given for poor plant health, visible signs of fungal infection,poor plant intactness characterized by missing leaves, tassels, or othervegetative parts, or a combination of these traits.

Herbicide resistant or tolerant. A plant containing anyherbicide-resistant gene or any DNA molecule or construct (or replicatethereof) which is not naturally occurring in the plant which results inincrease tolerance to any herbicide including, imidazoline,sulfonylurea, glyphosate, glufosinate, L-phosphinothricin, triazine andbenzonitrile. For purposes of this definition, a DNA molecule orconstruct shall be considered to be naturally occurring if it exists ina plant at a high enough frequency to provide herbicide resistancewithout further selection and/or if it has not been produced as a resultof tissue culture selection, mutagenesis, genetic engineering usingrecombinant DNA techniques or other in vitro or in vivo modification tothe plant.

Inbreeding depression. The inbreeding depression is the loss ofperformance of the inbreds due to the effect of inbreeding, i.e., due tothe mating of relatives or to self-pollination. It increases thehomozygous recessive alleles leading to plants which are weaker andsmaller and having other less desirable traits.

Late plant greenness. Similar to a stay green rating. This is a visualassessment given at around the dent stage but typically a few weeksbefore harvest to characterize the degree of greenness left in theleaves. Plants are rated from 1 (poorest) to 9 (best) with poorer scoresgiven for plants that have more non-green leaf tissue typically due toearly senescence or from disease.

MN RM. This represents the Minnesota Relative Maturity Rating (MN RM)for the hybrid and is based on the harvest moisture of the grainrelative to a standard set of checks of previously determined MN RMrating. Regression analysis is used to compute this rating.

Moisture. The moisture is the actual percentage moisture of the grain atharvest.

Plant cell. Plant cell, as used herein includes plant cells whetherisolated, in tissue culture, or incorporated in a plant or plant part.

Plant habit. This is a visual assessment assigned during the latevegetative to early reproductive stages to characterize the plant's leafhabit. It ranges from decumbent with leaves growing horizontally fromthe stalk to a very upright leaf habit, with leaves growing nearvertically from the stalk.

Plant height. This is a measure of the height of the hybrid from theground to the tip of the tassel, and is measured in centimeters.

Plant intactness. This is a visual assessment assigned to a hybrid orinbred at or close to harvest to indicate the degree that the plant hassuffered disintegration through the growing season. Plants are ratedfrom 1 (poorest) to 9 (best) with poorer scores given for plants thathave more of their leaf blades missing.

Plant part. As used herein, the term “plant part” includes any part ofthe plant including but not limited to leaves, stems, roots, seeds,grains, embryos, pollens, ovules, flowers, ears, cobs, husks, stalks,root tips, anthers, silk, tissue, cells and the like.

Pollen shed. This is a visual rating assigned at flowering to describethe abundance of pollen produced by the anthers. Inbreds are rated 1(poorest) to 9 (best) with the best scores for inbreds with tassels thatshed more pollen during anthesis.

Post-anthesis root lodging. This is a percentage plants that root lodgeafter anthesis: plants that lean from the vertical axis at anapproximately 30° angle or greater.

Pre-anthesis brittle snapping. This is a percentage of “snapped” plantsfollowing severe winds prior to anthesis.

Pre-anthesis root lodging. This is a percentage plants that root lodgeprior to anthesis: plants that lean from the vertical axis at anapproximately 30° angle or greater.

Predicted RM. This trait for a hybrid, predicted relative maturity (RM),is based on the harvest moisture of the grain. The relative maturityrating is based on a known set of checks and utilizes conventionalmaturity such as the Comparative Relative Maturity Rating System or itssimilar, the Minnesota Relative Maturity Rating System.

Quantitative trait loci (QTL). Quantitative trait loci refer to geneticloci that control to some degree numerically representable traits thatare usually continuously distributed.

RBDHV, CCR1, CRW2-1. RBDHV, CCR1 or CRW2-1 refers to MON88017, alsoknown as MON88017CCR1, is the transgenic event that, when expressed inmaize, allows the use of glyphosate as a weed control agent. Inaddition, this event produces an endotoxin that is efficacious againstthe corn root worm, Diabrotica virgifera, and certain other Coleopteranlarvae.

Regeneration. Regeneration refers to the development of a plant fromtissue culture.

RMQKZ, RMQKC, RMQKD. RMQKZ, RMQKC, or RMQKD refers to a combination ofMon88017 (see below) and Mon89034 transgenes for insect resistance andgyphosate tolerance. Mon89034 is a transgenic event expressed in maize,that produces an endotoxin that is efficacious against the European cornborer, Ostrinia nubilalis and certain other Lepidopteran larvae.

RHTTZ, RR2. RHTZZ and RR2 refers to MON603, also known as MON603RR2,better known as NK603, is the designation for the transgenic event that,when expressed in maize, allows the use of glyphosate as a weed controlagent on the crop. Another transgenic event, GA21, when expressed inmaize, also allows the use of glyphosate as a weed control agent on thecrop.

Root lodging. The root lodging is the percentage of plants that rootlodge; i.e., those that lean from the vertical axis at an approximate30° angle or greater would be counted as root lodged.

Seed quality. This is a visual rating assigned to the kernels of theinbred. Kernels are rated 1 (poorest) to 9 (best) with poorer scoresgiven for kernels that are very soft and shriveled with splitting of thepericarp visible and better scores for fully formed kernels.

Seedling vigor. This is the vegetative growth after emergence at theseedling stage, approximately five leaves.

Silking ability. This is a visual assessment given during flowering.Plants are rated on the amount and timing of silk production. Plants arerated from 1 (poorest) to 9 (best) with poorer scores given for plantsthat produce very little silks that are delayed past pollen shed.

Single gene converted. Single gene converted or conversion plants refersto plants which are developed by a plant breeding technique calledbackcrossing wherein essentially all the morphological and physiologicalcharacteristics of an inbred are recovered in addition to the singlegene transferred into the inbred via the backcrossing technique or viagenetic engineering. This also includes multiple transference of singlegenes.

Stalk lodging. This is the percentage of plants that stalk lodge, i.e.,stalk breakage, as measured by either natural lodging or pushing thestalks and determining the percentage of plants that break off below theear. This is a relative rating of a hybrid to other hybrids forstandability.

Standability. A term referring to the how well a plant remains uprighttowards the end of the growing season. Plants with excessive stalkbreakage and/or root lodging would be considered to have poorstandability.

Stay green. Stay green is the measure of plant health near the time ofblack layer formation (physiological maturity). A high score indicatesbetter late-season plant health.

Transgenic. Where an inbred line has been converted to contain one ormore transgenes by single gene conversion or by direct transformation.

Variety. A plant variety as used by one skilled in the art of plantbreeding means a plant grouping within a single botanical taxon of thelowest known rank which can be defined by the expression of thecharacteristics resulting from a given genotype or combination ofphenotypes, distinguished from any other plant grouping by theexpression of at least one of the said characteristics and considered asa unit with regard to its suitability for being propagated unchanged(International Convention for the Protection of New Varieties ofPlants).

Yield (Bushels/Acre). The yield is the actual yield of the grain atharvest adjusted to 15.5% moisture.

ZKDDZ. ZKDDZ refers to MON810, also known as MON810Bt or BT1, is thedesignation given by the Monsanto Company (St. Louis, Mo.) for thetransgenic event that, when expressed in maize, produces an endotoxinthat is efficacious against the European corn borer, Ostrinia nubilalisand certain other Lepidopteran larvae.

DETAILED DESCRIPTION OF THE INVENTION

Inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34,CB39, II15, II17, IM5, LK1, and MM65 is a corn inbred with superiorcharacteristics, and provide very good parental lines in crosses forproducing first generation (F₁) hybrid corn. Inbred corn line BB208,BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5,LK1, and MM65 is best adapted to the East, Central, South and Westernregions of the United States Corn Belt in the zones that are commonlyreferred to as Zones 5, 6 and 7. Hybrids that are adapted to thesematurity zones can be grown on a significant number of acres as itrelates to the total of the USA corn acres.

BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17,IM5, LK1, and MM65 is an inbred corn line with high yield potential inhybrids. Hybrids with inbred corn line BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 as oneparental line produce uniform, consistent sized ears with a high kernelrow number and heavy, hard-textured kernels. Often these hybridcombinations result in plants which are appreciably better than averagefor stalk strength, grain yield, and test weight when compared to inbredlines of similar maturity and geographical adaptability. Some of thecriteria used to select ears in various generations include: yield,yield to harvest moisture ratio, stalk quality, root quality, diseasetolerance with emphasis on grey leaf spot, test weight, late seasonplant greenness, late season plant intactness, ear retention, earheight, pollen shedding ability, silking ability, and corn borertolerance. During the development and selection of the line, crosseswere made to inbred testers for the purpose of estimating the line'sgeneral and specific combining ability, and evaluations were run by theAmes, Iowa Research Station. The inbred was evaluated further as a lineand in numerous crosses by the Ames station and other research stationsacross the Corn Belt. The inbred has proven to have an excellentcombining ability in hybrid combinations.

Inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34,CB39, II15, II17, IM5, LK1, and MM65 has shown uniformity and stabilityfor the traits, within the limits of environmental influence for thetraits. These lines have been increased with continued observation foruniformity of plant type. inbred corn line BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 has thefollowing morphologic and other characteristics (based primarily on datacollected at Ames, Iowa, unless otherwise specified).

BB208

TABLE 1A VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: BB208 is a yellow, dent corn inbred 2. Region where developed:Ames, IA 3. Maturity: Heat Units From planting to 50% of plants in silk:1562 GDD (Growing Degree Days) From planting to 50% of plants in pollen:1507 GDD Plant: 1. Plant height to tassel tip: 197.5 cm 2. Ear height tobase of top ear: 57.0 cm 3. Average length of top ear internode: 14.0 cm4. Average number of tillers: 0 5. Average number of ears per stalk: 1.66. Anthocyanin of brace roots: Strongly present Leaf: 1. Width of earnode leaf: 6.7 cm 2. Length of ear node leaf: 82.2 cm 3. Number ofleaves above top ear: 6.5 4. Leaf angle (from 2nd leaf above ear atanthesis to stalk above leaf): 58° 5. Leaf color: Greenish yellow,Munsell 7.5GY 4/4 6. Leaf sheath pubescence (Rated on scale from 1 =none to 9 = like peach fuzz): 2 7. Marginal waves (Rated on scale from 1= none to 9 = many): 7 8. Longitudinal creases (Rated on scale from 1 =none to 9 = many): 2 Tassel: 1. Number of lateral branches: 5.4 2.Branch angle from central spike: 57° 3. Tassel length (from top leafcollar to tassel top): 38.0 cm 4. Pollen shed (Rated on scale from 0 =male sterile to 9 = heavy shed): 4 5. Anther color: Yellow, Munsell 10Y7/10 6. Glume color: Greenish yellow, Munsell 2.5 GY 7/6 7. Tassel glumebands color: Absent Ear (Unhusked Data): 1. Silk color (3 days afteremergence): Greenish yellow, Munsell 2.5 GY 8.5/6 2. Fresh husk color(25 days after 50% silking): Greenish yellow, Munsell 5GY 7/8 3. Dryhusk color (65 days after 50% silking): Yellow, Munsell 5Y 9/2 4.Position of ear: n/a 5. Husk tightness (Rated on scale from 1 = veryloose to 9 = very tight): n/a 6. Husk extension at harvest: 2.2 cm Ear(Husked Ear Data): 1. Ear length: 10.1 cm 2. Ear diameter at mid-point:3.7 cm 3. Ear weight: 47.8 g 4. Number of kernel rows: 14 5. Rowalignment: Slightly crooked 6. Ear taper: Slight Kernel (Dried): 1.Kernel length: 10.5 mm 2. Kernel width: 7.0 mm 3. Kernel thickness: 5.0mm 4. Hard endosperm color: Yellowish red, Munsell 10YR 7/10 5.Endosperm type: Dent 6. Weight per 100 kernels (unsized sample): 25.3 gCob: 1. Cob diameter at mid-point: 21.4 mm 2. Cob color: Red, Munsell10R 6/6 Agronomic Traits: 1. Dropped ears (at 65 days after anthesis):0% 2. Pre-anthesis brittle snapping: 0% 3. Pre-anthesis root lodging: 0%4. Post-anthesis root lodging (at 65 days after harvest): N/A %BB209

TABLE 1B VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: BB209 is a yellow, dent corn inbred 2. Region where developed:Champaign, IL 3. Maturity: Heat Units From planting to 50% of plants insilk: 1511 From planting to 50% of plants in pollen: 1511 Plant: 1.Plant height to tassel tip: 200.0 cm 2. Ear height to base of top ear:55.0 cm 3. Average length of top ear internode: 13.0 cm 4. Averagenumber of tillers: 0.0 5. Average number of ears per stalk: 1.5 6.Anthocyanin of brace roots: Absent Leaf: 1. Width of ear node leaf: 9.0cm 2. Length of ear node leaf: 84.0 cm 3. Number of leaves above topear: 5.0 4. Leaf angle (from 2nd leaf above ear at anthesis to stalkabove leaf): 30° 5. Leaf color: Munsell 3-5GY3/2 6. Leaf sheathpubescence (Rated on scale from 1 = none to 9 = like peach fuzz): 3 7.Marginal waves (Rated on scale from 1 = none to 9 = many): 3 8.Longitudinal creases (Rated on scale from 1 = none to 9 = many): 2Tassel: 1. Number of lateral branches: 8.0 2. Branch angle from centralspike: 20° 3. Tassel length (from top leaf collar to tassel top): 47.0cm 4. Pollen shed (Rated on scale from 0 = male sterile to 9 = heavyshed): 8 5. Anther color: Munsell 3-10RP7/6 6. Glume color: Munsell2-5GY6/6 7. Tassel glume bands color: Absent Ear (Unhusked Data): 1.Silk color (3 days after emergence): Munsell 1-10Y9/8 2. Fresh huskcolor (25 days after 50% silking): Munsell 1-5GY8/10 3. Dry husk color(65 days after 50% silking): Munsell 1-5Y9/2 4. Position of ear: Upright5. Husk tightness (Rated on scale from 1 = very loose to 9 = verytight): 2 6. Husk extension at harvest: 2 (Medium, up to 8 cm) Ear(Husked Ear Data): 1. Ear length: 15.0 cm 2. Ear diameter at mid-point:4.3 cm 3. Ear weight: 146.0 g 4. Number of kernel rows: 14-16 5. Rowalignment: Straight 6. Ear taper: Average Kernel (Dried): 1. Kernellength: 12.0 mm 2. Kernel width: 7.0 mm 3. Kernel thickness: 4.0 mm 4.Hard endosperm color: Munsell 1-10YR8/10 5. Endosperm type: Dent 6.Weight per 100 kernels (unsized sample): 31.0 g Cob: 1. Cob diameter atmid-point: 22.0 mm 2. Cob color: Dark Red Agronomic Traits: 1. Droppedears (at 65 days after anthesis): 0% 2. Pre-anthesis brittle snapping:0% 3. Pre-anthesis root lodging: 0%BB210

TABLE 1C VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: BB210 is a yellow, dent corn inbred 2. Region where developed:Champaign, IL 3. Maturity: Heat Units From planting to 50% of plants insilk: 1488 GDD From planting to 50% of plants in pollen: 1511 GDDPlant: 1. Plant height to tassel tip: 215.0 cm 2. Ear height to base oftop ear: 60.0 cm 3. Average length of top ear internode: 15.0 cm 4.Average number of tillers: 0.0 5. Average number of ears per stalk: 1.96. Anthocyanin of brace roots: Absent Leaf: 1. Width of ear node leaf:8.0 cm 2. Length of ear node leaf: 88.0 cm 3. Number of leaves above topear: 6.0 4. Leaf angle (from 2nd leaf above ear at anthesis to stalkabove leaf): 20° 5. Leaf color: Munsell 3-5GY3/2 6. Leaf sheathpubescence (Rated on scale from 1 = none to 9 = like peach fuzz): 4 7.Marginal waves (Rated on scale from 1 = none to 9 = many): 2 8.Longitudinal creases (Rated on scale from 1 = none to 9 = many): 2Tassel: 1. Number of lateral branches: 9.0 2. Branch angle from centralspike: 70° 3. Tassel length (from top leaf collar to tassel top): 49.0cm 4. Pollen shed (Rated on scale from 0 = male sterile to 9 = heavyshed): 7 5. Anther color: Munsell 3-10RP7/8 6. Glume color: Munsell2-5GY6/8 7. Tassel glume bands color: Absent Ear (Unhusked Data): 1.Silk color (3 days after emergence): Munsell 1-10Y9/8 2. Fresh huskcolor (25 days after 50% silking): Munsell 2-5GY7/8 3. Dry husk color(65 days after 50% silking): Munsell 1-5Y9/4 4. Position of ear: Pendent5. Husk tightness (Rated on scale from 1 = very loose to 9 = verytight): 3 6. Husk extension at harvest: 2 (Medium, up to 8 cm) Ear(Husked Ear Data): 1. Ear length: 15.0 cm 2. Ear diameter at mid-point:4.4 cm 3. Ear weight: 147.0 g 4. Number of kernel rows: 14-16 5. Rowalignment: Straight 6. Ear taper: Average Kernel (Dried): 1. Kernellength: 12.0 mm 2. Kernel width: 8.0 mm 3. Kernel thickness: 4.0 mm 4.Hard endosperm color: Munsell 1-10YR7/12 5. Endosperm type: Dent 6.Weight per 100 kernels (unsized sample): 34.0 g Cob: 1. Cob diameter atmid-point: 21.0 mm 2. Cob color: Dark Red Agronomic Traits: 1. Droppedears (at 65 days after anthesis): 0% 2. Pre-anthesis brittle snapping:0% 3. Pre-anthesis root lodging: 0% 4. Post-anthesis root lodging (at 65days after harvest): 0%BB211

TABLE 1D VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: BB211 is a yellow, dent corn inbred 2. Region where developed:Champaign, IL 3. Maturity: Heat Units From planting to 50% of plants insilk: 1488 GDD From planting to 50% of plants in pollen: 1488 GDDPlant: 1. Plant height to tassel tip: 225.0 cm 2. Ear height to base oftop ear: 65.0 cm 3. Average length of top ear internode: 13.0 cm 4.Average number of tillers: 0.0 5. Average number of ears per stalk: 1.76. Anthocyanin of brace roots: Absent Leaf: 1. Width of ear node leaf:8.0 cm 2. Length of ear node leaf: 90.0 cm 3. Number of leaves above topear: 7.0 4. Leaf angle (from 2nd leaf above ear at anthesis to stalkabove leaf): 15° 5. Leaf color: Munsell 3-5GY4/4 6. Leaf sheathpubescence (Rated on scale from 1 = none to 9 = like peach fuzz): 3 7.Marginal waves (Rated on scale from 1 = none to 9 = many): 4 8.Longitudinal creases (Rated on scale from 1 = none to 9 = many): 2Tassel: 1. Number of lateral branches: 8.0 2. Branch angle from centralspike: 20° 3. Tassel length (from top leaf collar to tassel top): 40.0cm 4. Pollen shed (Rated on scale from 0 = male sterile to 9 = heavyshed): 6 5. Anther color: Munsell 1-10Y8.5/8 6. Glume color: Munsell1-5GY8.5/6 7. Tassel glume bands color: Absent Ear (Unhusked Data): 1.Silk color (3 days after emergence): Munsell 1-10Y9/8 2. Fresh huskcolor (25 days after 50% silking): Munsell 2-5GY7/8 3. Dry husk color(65 days after 50% silking): Munsell 1-5Y9/4 4. Position of ear: Pendent5. Husk tightness (Rated on scale from 1 = very loose to 9 = verytight): 2 6. Husk extension at harvest: 3 (Long, 8-10 cm beyond ear tip)Ear (Husked Ear Data): 1. Ear length: 15.0 cm 2. Ear diameter atmid-point: 4.3 cm 3. Ear weight: 151.0 g 4. Number of kernel rows: 14-165. Row alignment: Straight 6. Ear taper: Average Kernel (Dried): 1.Kernel length: 12.0 mm 2. Kernel width: 7.0 mm 3. Kernel thickness: 4.0mm 4. Hard endosperm color: Munsell 1-10YR8/10 5. Endosperm type: Dent6. Weight per 100 kernels (unsized sample): 28.0 g Cob: 1. Cob diameterat mid-point: 20.0 mm 2. Cob color: Dark Red Agronomic Traits: 1.Dropped ears (at 65 days after anthesis): 0% 2. Pre-anthesis brittlesnapping: 0% 3. Pre-anthesis root lodging: 0% 4. Post-anthesis rootlodging (at 65 days after harvest): 0%BC146

TABLE 1E VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: BC146 is a yellow, dent corn inbred 2. Region where developed:Kirkland, Illinois, United States 3. Maturity: 111 Heat Units Fromplanting to 50% of plants in silk: 1461 GDD From planting to 50% ofplants in pollen: 1461 GDD Plant: 1. Plant height to tassel tip: 249.9cm 2. Ear height to base of top ear: 70.5 cm 3. Average length of topear internode: 14.1 cm 4. Average number of tillers: 0 5. Average numberof ears per stalk: 1.1 6. Anthocyanin of brace roots: Absent Leaf: 1.Width of ear node leaf: 9.2 cm 2. Length of ear node leaf: 80.7 cm 3.Number of leaves above top ear: 6.7 4. Leaf angle (from 2nd leaf aboveear at anthesis to stalk above leaf): 12.2° 5. Leaf color: Munsell 7.5GY3/4 Green 6. Leaf sheath pubescence (Rated on scale from 1 = none to 9 =like peach fuzz): 4 7. Marginal waves (Rated on scale from 1 = none to 9= many): 7 8. Longitudinal creases (Rated on scale from 1 = none to 9 =many): 7 Tassel: 1. Number of lateral branches: 6.3 2. Branch angle fromcentral spike: 35.8° 3. Tassel length (from top leaf collar to tasseltop): 49.1 cm 4. Pollen shed (Rated on scale from 0 = male sterile to 9= heavy shed): 7 5. Anther color: Munsell 2.5GY8/6 Green 6. Glume color:Munsell 2.5GY8/8 Green 7. Tassel glume bands color: Absent Ear (UnhuskedData): 1. Silk color (3 days after emergence): Munsell 2.5GY8/6 Green 2.Fresh husk color (25 days after 50% silking): Munsell 5GY7/8 Green 3.Dry husk color (65 days after 50% silking): Munsell 2.5Y8/8 Yellow 4.Position of ear: Upright 5. Husk tightness (Rated on scale from 1 = veryloose to 9 = very tight): 5 6. Husk extension at harvest: 2.1 cm ShortEar (Husked Ear Data): 1. Ear length: 13.3 cm 2. Ear diameter atmid-point: 46.4 mm 3. Ear weight: 134.4 g 4. Number of kernel rows:16-18 5. Row alignment: Slightly Curved 6. Ear taper: Average Kernel(Dried): 1. Kernel length: 13.4 mm 2. Kernel width: 8.9 mm 3. Kernelthickness: 4.2 mm 4. Hard endosperm color: Munsell 2.5Y8/10 5. Endospermtype: Dent 6. Weight per 100 kernels (unsized sample): 28.0 g Cob: 1.Cob diameter at mid-point: 23.7 mm 2. Cob color: Munsell 5R6/8 RedAgronomic Traits: 1. Dropped ears (at 65 days after anthesis): 0% 2.Pre-anthesis brittle snapping: 0% 3. Pre-anthesis root lodging: 0% 4.Post-anthesis root lodging (at 65 days after harvest): 0%BBC147

TABLE 1F VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: BC147 is a yellow, dent corn inbred 2. Region where developed:Kirkland, Illinois, United States 3. Maturity: 112 Heat Units Fromplanting to 50% of plants in silk: 1461 GDD From planting to 50% ofplants in pollen: 1438 GDD Plant: 1. Plant height to tassel tip: 241.7cm 2. Ear height to base of top ear: 76.2 cm 3. Average length of topear internode: 15.4 cm 4. Average number of tillers: 0 5. Average numberof ears per stalk: 1.7 6. Anthocyanin of brace roots: Faint Leaf: 1.Width of ear node leaf: 9.8 cm 2. Length of ear node leaf: 85.0 cm 3.Number of leaves above top ear: 6.2 4. Leaf angle (from 2nd leaf aboveear at anthesis to stalk above leaf): 14.8° 5. Leaf color: Munsell7.5GY3/4 Green 6. Leaf sheath pubescence (Rated on scale from 1 = noneto 9 = like peach fuzz): 6 7. Marginal waves (Rated on scale from 1 =none to 9 = many): 3 8. Longitudinal creases (Rated on scale from 1 =none to 9 = many): 4 Tassel: 1. Number of lateral branches: 6.3 2.Branch angle from central spike: 37.3° 3. Tassel length (from top leafcollar to tassel top): 44.1 cm 4. Pollen shed (Rated on scale from 0 =male sterile to 9 = heavy shed): 7 5. Anther color: Munsell 2.5GY8/6Green 6. Glume color: Munsell 2.5GY8/6 Green 7. Tassel glume bandscolor: Absent Ear (Unhusked Data): 1. Silk color (3 days afteremergence): Munsell 2.5GY8/8 Green 2. Fresh husk color (25 days after50% silking): Munsell 2.5GY8/8 Green 3. Dry husk color (65 days after50% silking): Munsell 2.5Y8/6 Yellow 4. Position of ear: Upright 5. Husktightness (Rated on scale from 1 = very loose to 9 = very tight): 6 6.Husk extension at harvest: 2.2 cm Short Ear (Husked Ear Data): 1. Earlength: 14.0 cm 2. Ear diameter at mid-point: 45.9 mm 3. Ear weight:134.3 g 4. Number of kernel rows: 16 5. Row alignment: Slightly Curved6. Ear taper: Slight Kernel (Dried): 1. Kernel length: 12.3 mm 2. Kernelwidth: 8.7 mm 3. Kernel thickness: 4.3 mm 4. Hard endosperm color:Munsell 2.5Y7/10 5. Endosperm type: Dent 6. Weight per 100 kernels(unsized sample): 26.8 g Cob: 1. Cob diameter at mid-point: 23.0 mm 2.Cob color: Munsell 5R4/6 Red Agronomic Traits: 1. Dropped ears (at 65days after anthesis): 0% 2. Pre-anthesis brittle snapping: 0% 3.Pre-anthesis root lodging: 0% 4. Post-anthesis root lodging (at 65 daysafter harvest): 0%CB21

TABLE 1G VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: CB21 is a yellow, dent corn inbred 2. Region where developed:Lebanon, Indiana, USA 3. Maturity: Heat Units From planting to 50% ofplants in silk: 1565 GDD From planting to 50% of plants in pollen: 1573GDD Plant: 1. Plant height to tassel tip: 181.5 cm 2. Ear height to baseof top ear: 77.5 cm 3. Average length of top ear internode: 12.75 cm 4.Average number of tillers: 0 5. Average number of ears per stalk: 2.0 6.Anthocyanin of brace roots: purple Leaf: 1. Width of ear node leaf: 8.25cm 2. Length of ear node leaf: 68.5 cm 3. Number of leaves above topear: 5.0 4. Leaf angle (from 2nd leaf above ear at anthesis to stalkabove leaf): 25° 5. Leaf color: 7.5 GY 4/2 Munsell 6. Leaf sheathpubescence (Rated on scale from 1 = none to 9 = like peach fuzz): 1 7.Marginal waves (Rated on scale from 1 = none to 9 = many): 4 8.Longitudinal creases (Rated on scale from 1 = none to 9 = many): 3Tassel: 1. Number of lateral branches: 5 2. Branch angle from centralspike: 28° 3. Tassel length (from top leaf collar to tassel top): 33 cm4. Pollen shed (Rated on scale from 0 = male sterile to 9 = heavy shed):5 5. Anther color: Munsell 5 YR 6/8 6. Glume color: Munsell 7.5 GY 6/67. Tassel glume bands color: Absent Ear (Unhusked Data): 1. Silk color(3 days after emergence): Munsell 2.5 GY 8/10 2. Fresh husk color (25days after 50% silking): Munsell 5GY 6/8 3. Dry husk color (65 daysafter 50% silking): Munsell 2.5Y 8/6 4. Position of ear: verticalPendent 5. Husk tightness (Rated on scale from 1 = very loose to 9 =very tight): 8 6. Husk extension at harvest: 6.98 cm Ear (Husked EarData): 1. Ear length: 11.27 cm 2. Ear diameter at mid-point: 3.89 cm 3.Ear weight: 86.28 g 4. Number of kernel rows: 16 5. Row alignment:Straight 6. Ear taper: conico-cylindrical Kernel (Dried): 1. Kernellength: 11.25 mm 2. Kernel width: 7.7 mm 3. Kernel thickness: 3.175 mm4. Hard endosperm color: Grey 5. Endosperm type: Dent 6. Weight per 100kernels (unsized sample): 22.9 g Cob: 1. Cob diameter at mid-point:19.05 mm 2. Cob color: Red, Munsell 2.5 YR 4/6 Munsell AgronomicTraits: 1. Dropped ears (at 65 days after anthesis): 0% 2. Pre-anthesisbrittle snapping: 0% 3. Pre-anthesis root lodging: 0% 4. Post-anthesisroot lodging (at 65 days after harvest): 0%CB34

TABLE 1H VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: CB34 is a yellow, dent corn inbred 2. Region where developed:Champaign, IL 3. Maturity: Heat Units From planting to 50% of plants insilk: 1511 GDD From planting to 50% of plants in pollen: 1536 GDDPlant: 1. Plant height to tassel tip: 225.0 cm 2. Ear height to base oftop ear: 75.0 cm 3. Average length of top ear internode: 15.0 cm 4.Average number of tillers: 0.0 5. Average number of ears per stalk: 1.86. Anthocyanin of brace roots: Absent Leaf: 1. Width of ear node leaf:10.0 cm 2. Length of ear node leaf: 80.0 cm 3. Number of leaves abovetop ear: 6.0 4. Leaf angle (from 2nd leaf above ear at anthesis to stalkabove leaf): 25° 5. Leaf color: Munsell 3-5GY4/4 6. Leaf sheathpubescence (Rated on scale from 1 = none to 9 = like peach fuzz): 2 7.Marginal waves (Rated on scale from 1 = none to 9 = many): 4 8.Longitudinal creases (Rated on scale from 1 = none to 9 = many): 2Tassel: 1. Number of lateral branches: 4.0 2. Branch angle from centralspike: 15° 3. Tassel length (from top leaf collar to tassel top): 40.0cm 4. Pollen shed (Rated on scale from 0 = male sterile to 9 = heavyshed): 6 5. Anther color: Munsell 3-10RP7/8 6. Glume color: Munsell1-5GY9/6 7. Tassel glume bands color: Absent Ear (Unhusked Data): 1.Silk color (3 days after emergence): Munsell 1-10Y9/8 2. Fresh huskcolor (25 days after 50% silking): Munsell 1-5GY8/10 3. Dry husk color(65 days after 50% silking): Munsell 1-5Y8/6 4. Position of ear: Upright5. Husk tightness (Rated on scale from 1 = very loose to 9 = verytight): 2 6. Husk extension at harvest: Long (8-10 cm) Ear (Husked EarData): 1. Ear length: 14.0 cm 2. Ear diameter at mid-point: 4.3 cm 3.Ear weight: 128.0 g 4. Number of kernel rows: 14-16 5. Row alignment:Straight 6. Ear taper: Average Kernel (Dried): 1. Kernel length: 12.0 mm2. Kernel width: 8.0 mm 3. Kernel thickness: 4.0 mm 4. Hard endospermcolor: Munsell 2-5Y8/12 5. Endosperm type: Dent 6. Weight per 100kernels (unsized sample): 33.0 g Cob: 1. Cob diameter at mid-point: 21.0mm 2. Cob color: Red Agronomic Traits: 1. Dropped ears (at 65 days afteranthesis): 0% 2. Pre-anthesis brittle snapping: 0% 3. Pre-anthesis rootlodging: 0% 4. Post-anthesis root lodging (at 65 days after harvest): 0%CB39

TABLE 1I VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: CB39 is a yellow, dent corn inbred 2. Region where developed: FortBranch, Indiana, USA 3. Maturity: 110 RM Heat Units From planting to 50%of plants in silk: 1557.5 GDD From planting to 50% of plants in pollen:1494.0 GDD Plant: 1. Plant height to tassel tip: 184.0 cm 2. Ear heightto base of top ear: 54.0 cm 3. Average length of top ear internode: 12.3cm 4. Average number of tillers: 0 5. Average number of ears per stalk:1.7 6. Anthocyanin of brace roots: Faint Leaf: 1. Width of ear nodeleaf: 8.63 cm 2. Length of ear node leaf: 75.2 cm 3. Number of leavesabove top ear: 5.7 4. Leaf angle (from 2nd leaf above ear at anthesis tostalk above leaf): 49.5° 5. Leaf color: Munsell 5GY 4/4 6. Leaf sheathpubescence (Rated on scale from 1 = none to 9 = like peach fuzz): 5.5 7.Marginal waves (Rated on scale from 1 = none to 9 = many): 5 8.Longitudinal creases (Rated on scale from 1 = none to 9 = many): 6.5Tassel: 1. Number of lateral branches: 5.2 2. Branch angle from centralspike: 52.5° 3. Tassel length (from top leaf collar to tassel top): 42.5cm 4. Pollen shed (Rated on scale from 0 = male sterile to 9 = heavyshed): 6.3 5. Anther color: Munsell 2.5 GY 8/8 6. Glume color: Munsell2.5 GY 6/6 7. Tassel glume bands color: Absent Ear (Unhusked Data): 1.Silk color (3 days after emergence): Munsell 2.5 GY 8/6 2. Fresh huskcolor (25 days after 50% silking): Munsell 5GY 7/8 3. Dry husk color (65days after 50% silking): Munsell 5Y 8/10 4. Position of ear: Upright 5.Husk tightness (Rated on scale from 1 = very loose to 9 = very tight):2.5 6. Husk extension at harvest: Short (exposed) Ear (Husked EarData): 1. Ear length: 15.8 cm 2. Ear diameter at mid-point: 42.4 cm 3.Ear weight: 131.42 g 4. Number of kernel rows: 16 5. Row alignment:Straight 6. Shank length: 14.15 cm 7. Ear taper: Average Kernel(Dried): 1. Kernel length: 11.55 mm 2. Kernel width: 8.1 mm 3. Kernelthickness: 4.1 mm 4. Hard endosperm color: Munsell 2.5 Y 8/10 5.Endosperm type: Normal Starch 6. Weight per 100 kernels (unsizedsample): 30.7 g Cob: 1. Cob diameter at mid-point: 51.5 mm 2. Cob color:Red, Munsell 10 R 4/4 Agronomic Traits: 1. Dropped ears (at 65 daysafter anthesis): 0% 2. Pre-anthesis brittle snapping: 0% 3. Pre-anthesisroot lodging: 0% 4. Post-anthesis root lodging (at 65 days afterharvest): 0%II15

TABLE 1J VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: II15 is a yellow, dent corn inbred 2. Region where developed:Morris, Minnesota 3. Maturity: Heat Units: From planting to 50% ofplants in silk: 1357.8 GDD From planting to 50% of plants in pollen:1320.6 GDD Plant: 1. Plant height to tassel tip: 201.5 cm 2. Ear heightto base of top ear: 76.0 cm 3. Average length of top ear internode: 17.0cm 4. Average number of tillers: 0 5. Average number of ears per stalk:1.1 (average of 10 plants) 6. Anthocyanin of brace roots: Yes Leaf: 1.Width of ear node leaf: 6.6 cm 2. Length of ear node leaf: 75.6 cm 3.Number of leaves above top ear: 5.3 (average of 10 plants) 4. Leaf angle(from 2^(nd) leaf above ear at anthesis to stalk above leaf): 15-20° 5.Leaf color: 5GY 5/4 6. Leaf sheath pubescence (rated on scale from 1 =none, to 9 = like peach fuzz): 4 7. Marginal waves (rated on scale from1 = none, to 9 = many): 3 8. Longitudinal creases (rated on scale from 1= none, to 9 = many): 3 Tassel: 1. Number of lateral branches: 7.1(average of 10 plants) 2. Branch angle from central spike: 20-30° 3.Tassel length (from top leaf collar to tassel top): 45.25 cm 4. Pollenshed (rated on scale from 0 = male sterile, to 9 = heavy shed): 3 5.Anther color: 5Y 7/6 6. Glume color: 5R 4/8-2.5GY 6/6 7. Tassel glumebands color: Absent Ear (Unhusked Data): 1. Silk color (3 days afteremergence): 2.5GY 8/6 2. Fresh husk color (25 days after 50% silking):2.5GY 8/8 3. Dry husk color (65 days after 50% silking): 2.5Y 8/2 4.Position of ear: some upright, some tilting 5. Husk tightness (rated onscale from 1 = very loose, to 9 = very tight): 6.6 6. Husk extension atharvest: to the tip Ear (Husked Ear Data): 1. Ear length: 14.15 cm 2.Ear diameter at mid-point: 35.6 mm 3. Ear weight: 99.5 g 4. Number ofkernel rows: 13.1 5. Row alignment: not straight 6. Shank length: 14.6cm 7. Ear taper: tapered Kernel (Dried): 1. Kernel length: 12.75 mm 2.Kernel width: 8.7 mm 3. Kernel thickness: 4.55 mm 4. Hard endospermcolor: 7.5YR 7/10 5. Endosperm type: dent 6. Weight per 100 kernels(unsized sample): 34.0 g Cob: 1. Cob diameter at mid-point: 20.8 mm 2.Cob color: 5YR 7/6 Argonomic Traits: 1. Dropped ears (at 65 days afteranthesis): 0 2. Pre-anthesis brittle snapping: 0.0% 3. Pre-anthesis rootlodging: 0.0% 4. Post-anthesis root lodging (at 65 days after anthesis):0.0%II17

TABLE 1K VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: II17 is a yellow, dent corn inbred 2. Region where developed:Kenyon, Minnesota, USA 3. Maturity: Heat Units From planting to 50% ofplants in silk: 1470 From planting to 50% of plants in pollen: 1470Plant: 1. Plant height to tassel tip: 193 cm 2. Ear height to base oftop ear: 101 cm 3. Average length of top ear internode: N/A 4. Averagenumber of tillers: 0 5. Average number of ears per stalk: 1 6.Anthocyanin of brace roots: green w/purple banding Leaf: 1. Width of earnode leaf: N/A 2. Length of ear node leaf: N/A 3. Number of leaves abovetop ear: N/A 4. Leaf angle (from 2nd leaf above ear at anthesis to stalkabove leaf): N/A 5. Leaf color: N/A 6. Leaf sheath pubescence (Rated onscale from 1 = none to 9 = like peach fuzz): N/A 7. Marginal waves(Rated on scale from 1 = none to 9 = many): 9 8. Longitudinal creases(Rated on scale from 1 = none to 9 = many): 3 Tassel: 1. Number oflateral branches: N/A 2. Branch angle from central spike: N/A 3. Tassellength (from top leaf collar to tassel top): N/A 4. Pollen shed (Ratedon scale from 0 = male sterile to 9 = heavy shed): 6 5. Anther color:N/A 6. Glume color: N/A 7. Tassel glume bands color: N/A Ear (UnhuskedData): 1. Silk color (3 days after emergence): N/A 2. Fresh husk color(25 days after 50% silking): N/A 3. Dry husk color (65 days after 50%silking): N/A 4. Position of ear: N/A 5. Husk tightness (Rated on scalefrom 1 = very loose to 9 = very tight): 5 6. Husk extension at harvest:N/A Ear (Husked Ear Data): 1. Ear length: N/A 2. Ear diameter atmid-point: N/A 3. Ear weight: N/A 4. Number of kernel rows: N/A 5. Rowalignment: straight 6. Ear taper: N/A Kernel (Dried): 1. Kernel length:10 mm 2. Kernel width: 8 mm 3. Kernel thickness: 8 mm 4. Hard endospermcolor: N/A 5. Endosperm type: Dent 6. Weight per 100 kernels (unsizedsample): 21 g Cob: 1. Cob diameter at mid-point: N/A 2. Cob color: RedAgronomic Traits: 1. Dropped ears (at 65 days after anthesis): 0% 2.Pre-anthesis brittle snapping: 0% 3. Pre-anthesis root lodging: 0%IM5

TABLE 1L VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: IM5 is a yellow, dent corn inbred 2. Region where developed:Morris, Minnesota 3. Maturity: Heat Units: From planting to 50% ofplants in silk: 1446.5  From planting to 50% of plants in pollen:1416.35 Plant: 1. Plant height to tassel tip: 212.5 cm 2. Ear height tobase of top ear: 81.0 cm 3. Average length of top ear internode: 12.65cm 4. Average number of tillers: 0 5. Average number of ears per stalk:1.6 (average of 10 plants) 6. Anthocyanin of brace roots: Yes Leaf: 1.Width of ear node leaf: 7.05 cm 2. Length of ear node leaf: 83.35 cm 3.Number of leaves above top ear: 6 4. Leaf angle (from 2^(nd) leaf aboveear at anthesis to stalk above leaf): 10-20° 5. Leaf color: 5GY 5/4 6.Leaf sheath pubescence (rated on scale from 1 = none, to 9 = like peachfuzz): 3 7. Marginal waves (rated on scale from 1 = none, to 9 = many):2 8. Longitudinal creases (rated on scale from 1 = none, to 9 = many): 2Tassel: 1. Number of lateral branches: 8 2. Branch angle from centralspike: 10-15° 3. Tassel length (from top leaf collar to tassel top):46.5 cm 4. Pollen shed (rated on scale from 0 = male sterile, to 9 =heavy shed): 7 5. Anther color: 5Y 8/8 6. Glume color: 2.5GY 7/6-2.5R4/8 7. Tassel glume bands color: Absent Ear (Unhusked Data): 1. Silkcolor (3 days after emergence): 2.5GY 8/6 2. Fresh husk color (25 daysafter 50% silking): 2.5GY 8/6 3. Dry husk color (65 days after 50%silking): 2.5Y 8/4 4. Position of ear: mostly upright, some tilting 5.Husk tightness (rated on scale from 1 = very loose, to 9 = very tight):2.3 6. Husk extension at harvest: past the ear Ear (Husked Ear Data): 1.Ear length: 13.3 cm 2. Ear diameter at mid-point: 42.7 mm 3. Ear weight:111.2 g 4. Number of kernel rows: 16 5. Row alignment: not straight 6.Shank length: 10.2 cm 7. Ear taper: average Kernel (Dried): 1. Kernellength: 11.7 mm 2. Kernel width: 7.25 mm 3. Kernel thickness: 3.65 mm 4.Hard endosperm color: 5YR 6/10 5. Endosperm type: dent 6. Weight per 100kernels (unsized sample): 26.0 g Cob: 1. Cob diameter at mid-point: 22.3mm 2. Cob color: 2.5YR 5/8 Argonomic Traits: 1. Dropped ears (at 65 daysafter anthesis): 0 2. Pre-anthesis brittle snapping: 0.0% 3.Pre-anthesis root lodging: 0.0% 4. Post-anthesis root lodging (at 65days after anthesis): 0LK1

TABLE 1M VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: LK1 is a yellow, dent corn inbred 2. Region where developed: Ames,IA 3. Maturity: Heat Units: From planting to 50% of plants in silk: 1444From planting to 50% of plants in pollen: 1414 Plant: 1. Plant height totassel tip: 200.0 cm 2. Ear height to base of top ear: 65.5 cm 3.Average length of top ear internode: 13.0 cm 4. Average number oftillers: 0 5. Average number of ears per stalk: 1.1 6. Anthocyanin ofbrace roots: Absent Leaf: 1. Width of ear node leaf: 8.0 cm 2. Length ofear node leaf: 68.6 cm 3. Number of leaves above top ear: 5.0 4. Leafangle (from 2nd leaf above ear at anthesis to stalk above leaf): N/A 5.Leaf color: Yellowish green, Munsell 10GY 6/4 6. Leaf sheath pubescence(Rated on scale from 1 = none to 9 = like peach fuzz): 8 7. Marginalwaves (Rated on scale from 1 = none to 9 = many): 4 8. Longitudinalcreases (Rated on scale from 1 = none to 9 = many): 6 Tassel: 1. Numberof lateral branches: 5.4 2. Branch angle from central spike: 42.5° 3.Tassel length (from top leaf collar to tassel top): 40.0 cm 4. Pollenshed (Rated on scale from 0 = male sterile to 9 = heavy shed): 3 5.Anther color: Olive, Munsell 5Y 6/8 6. Glume color: Yellow green,Munsell 2.5GY 7/6 7. Tassel glume bands color: Absent Ear (UnhuskedData): 1. Silk color (3 days after emergence): Pale yellowish green,Munsell 2.5 GY 8/10 2. Fresh husk color (25 days after 50% silking):Greenish yellow, Munsell 2.5GY 6/8 3. Dry husk color (65 days after 50%silking): Yellow, Munsell 2.5Y 8/6 4. Position of ear: Pendant 5. Husktightness (Rated on scale from 1 = very loose to 9 = very tight): 2 6.Husk extension at harvest: N/A Ear (Husked Ear Data): 1. Ear length:13.7 cm 2. Ear diameter at mid-point: 4.1 cm 3. Ear weight: 87.9 g 4.Number of kernel rows: 14 5. Row alignment: Slightly crooked 6. Eartaper: None Kernel (Dried): 1. Kernel length: 11.2 mm 2. Kernel width:9.6 mm 3. Kernel thickness: 5.4 mm 4. Hard endosperm color: Yellow redMunsell 7.5YR 5/8 5. Endosperm type: Dent 6. Weight per 100 kernels(unsized sample): 33.4 g Cob: 1. Cob diameter at mid-point: 24.7 mm 2.Cob color: Red, Munsell 10R 4/6 Agronomic Traits: 1. Dropped ears (at 65days after anthesis): 0% 2. Pre-anthesis brittle snapping: 1.8% 3.Pre-anthesis root lodging: 0% 4. Post-anthesis root lodging (at 65 daysafter harvest): 0%MM65

TABLE 1N VARIETY DESCRIPTION INFORMATION General Plant Information: 1.Type: MM65 is a yellow, dent corn inbred 2. Region where developed: FortBranch, Indiana, USA 3. Maturity: 112 RM Heat Units: From planting to50% of plants in silk: 1570.5 GDD From planting to 50% of plants inpollen: 1570.5 GDD Plant: 1. Plant height to tassel tip: 201 cm 2. Earheight to base of top ear: 50.5 cm 3. Average length of top earinternode: 7.2 cm 4. Average number of tillers: 0 5. Average number ofears per stalk: 1.3 6. Anthocyanin of brace roots: Absent Leaf: 1. Widthof ear node leaf: 9.5 cm 2. Length of ear node leaf: 68.2 cm 3. Numberof leaves above top ear: 5.9 4. Leaf angle (from 2nd leaf above ear atanthesis to stalk above leaf): 50° 5. Leaf color: Munsell 7.5 GY 3/4 6.Leaf sheath pubescence (Rated on scale from 1 = none to 9 = like peachfuzz): 1 7. Marginal waves (Rated on scale from 1 = none to 9 = many):2.5 8. Longitudinal creases (Rated on scale from 1 = none to 9 = many):3 Tassel: 1. Number of lateral branches: 4.2 2. Branch angle fromcentral spike: 67° 3. Tassel length (from top leaf collar to tasseltop): 38.8 cm 4. Pollen shed (Rated on scale from 0 = male sterile to 9= heavy shed): 8 5. Anther color: Munsell 2.5 GY 8/6 6. Glume color:Munsell 5 GY 6/6 7. Tassel glume bands color: Absent Ear (UnhuskedData): 1. Silk color (3 days after emergence): Munsell 2.5 GY 6/10 2.Fresh husk color (25 days after 50% silking): Munsell 5 GY 6/6 3. Dryhusk color (65 days after 50% silking): Munsell 5Y 8/10 4. Position ofear: Horizontal 5. Husk tightness (Rated on scale from 1 = very loose to9 = very tight): 1 6. Husk extension at harvest: Short Ear (Husked EarData): 1. Ear length: 17.9 cm 2. Ear diameter at mid-point: 40.7 mmm 3.Ear weight: 119.76 g 4. Number of kernel rows: 14 5. Row alignment:Straight 6. Ear taper: Slight Kernel (Dried): 1. Kernel length: 11.2 mm2. Kernel width: 8.5 mm 3. Kernel thickness: 4.5 mm 4. Hard endospermcolor: Munsell 2.5 Y 8/10 5. Endosperm type: Normal Starch 6. Weight per100 kernels (unsized sample): 29.95 g Cob: 1. Cob diameter at mid-point:25.7 mm 2. Cob color: Munsell N 9.3 Agronomic Traits: 1. Dropped ears(at 65 days after anthesis): 0% 2. Pre-anthesis brittle snapping: 0% 3.Pre-anthesis root lodging: 5.5% 4. Post-anthesis root lodging (at 65days after harvest): 0%

Further Embodiments of the Invention

This invention is also directed to methods for producing a corn plant bycrossing a first parent corn plant with a second parent corn plantwherein either the first or second parent corn plant is an inbred cornplant of inbred corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65. Further, both firstand second parent corn plants can come from the inbred corn line BB208,BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5,LK1, and MM65. When self-pollinated, or crossed with another inbred cornlines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15,II17, IM5, LK1, and MM65 plant, inbred corn lines BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65will be stable while when crossed with another, different corn line, anF₁ hybrid seed is produced. Such methods of hybridization andself-pollination of corn are well known to those skilled in the art ofcorn breeding.

An inbred corn line has been produced through several cycles ofself-pollination and is therefore to be considered as a homozygous line.An inbred line can also be produced though the dihaploid system whichinvolves doubling the chromosomes from a haploid plant thus resulting inan inbred line that is genetically stable (homozygous) and can bereproduced without altering the inbred line. A hybrid variety isclassically created through the fertilization of an ovule from an inbredparental line by the pollen of another, different inbred parental line.Due to the homozygous state of the inbred line, the produced gametescarry a copy of each parental chromosome. As both the ovule and thepollen bring a copy of the arrangement and organization of the genespresent in the parental lines, the genome of each parental line ispresent in the resulting F₁ hybrid, theoretically in the arrangement andorganization created by the plant breeder in the original parental line.

As long as the homozygosity of the parental lines is maintained, theresulting hybrid cross is stable. The F₁ hybrid is then a combination ofphenotypic characteristics issued from two arrangement and organizationof genes, both created by one skilled in the art through the breedingprocess.

Still further, this invention also is directed to methods for producingan inbred corn line BB208, BB209, BB210, BB211, BC146, BC147, CB21,CB34, CB39, II15, II17, IM5, LK1, and MM65-derived corn plant bycrossing inbred corn line BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 with a second cornplant and growing the progeny seed, and repeating the crossing andgrowing steps with the inbred corn line BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65-derivedplant from 0 to 7 times. Thus, any such methods using the inbred cornlines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15,II17, IM5, LK1, and MM65 are part of this invention: selfing,backcrosses, hybrid production, crosses to populations, and the like.All plants produced using inbred corn lines BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 as aparent are within the scope of this invention, including plants derivedfrom inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147, CB21,CB34, CB39, II15, II17, IM5, LK1, and MM65. Advantageously, the inbredcorn line is used in crosses with other, different, corn inbreds toproduce first generation (F₁) corn hybrid seeds and plants with superiorcharacteristics.

It should be understood that the inbred can, through routinemanipulation of cytoplasmic or other factors, be produced in amale-sterile form. Such embodiments are also contemplated within thescope of the present claims. As used herein, the term plant includesplant cells, plant protoplasts, plant cell tissue cultures from whichcorn plants can be regenerated, plant calli, plant clumps and plantcells that are intact in plants or parts of plants, such as embryos,pollen, ovules, flowers, kernels, seeds, ears, cobs, leaves, husks,stalks, roots, root tips, anthers, silk and the like.

Duncan, et al., (Planta, 1985, 165:322-332) indicates that 97% of theplants cultured that produced callus were capable of plant regeneration.Subsequent experiments with both inbreds and hybrids produced 91%regenerable callus that produced plants. In a further study in 1988,Songstad, et al. (Plant Cell Reports, 7:262-265) reports several mediaadditions that enhance regenerability of callus of two inbred lines.Other published reports also indicated that “nontraditional” tissues arecapable of producing somatic embryogenesis and plant regeneration. K. V.Rao et al., (Maize Genetics Cooperation Newsletter, 1986, 60:64-65)refer to somatic embryogenesis from glume callus cultures and B. V.Conger, et al. (Plant Cell Reports, 1987, 6:345-347) indicate somaticembryogenesis from the tissue cultures of corn leaf segments. Thus, itis clear from the literature that the state of the art is such thatthese methods of obtaining plants are routinely used and have a veryhigh rate of success.

Tissue culture of corn is also described in European Patent Application,publication 160,390 and in Green and Rhodes, Maize for BiologicalResearch, Plant Molecular Biology Association, Charlottesville, Va.,1982, 367-372. Thus, another aspect of this invention is to providecells which upon growth and differentiation produce corn plants havingthe physiological and morphological characteristics of inbred corn linesBB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15, II17,IM5, LK1, and MM65.

The utility of inbred corn lines BB208, BB209, BB210, BB211, BC146,BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 also extends tocrosses with other species. Commonly, suitable species will be of thefamily Graminaceae, and especially of the genera Zea, Tripsacum, Coix,Schlerachne, Polytoca, Chionachne, and Trilobachne, of the tribeMaydeae. Potentially suitable for crosses with BB208, BB209, BB210,BB211, BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65may be the various varieties of grain sorghum, Sorghum bicolor (L.)Moench.

With the advent of molecular biological techniques that have allowed theisolation and characterization of genes that encode specific proteinproducts, scientists in the field of plant biology developed a stronginterest in engineering the genome of plants to contain and expressforeign genes, or additional, or modified versions of native, orendogenous, genes (perhaps driven by different promoters) in order toalter the traits of a plant in a specific manner. Such foreignadditional and/or modified genes are referred to herein collectively as“transgenes.” Over the last fifteen to twenty years several methods forproducing transgenic plants have been developed, and the presentinvention, in particular embodiments, also relates to transformedversions of the claimed inbred line. An embodiment of the presentinvention comprises at least one transformation event in inbred cornlines BB208, BB209, BB210, BB211, BC146, BC147, CB21, CB34, CB39, II15,II17, IM5, LK1, and MM65.

Plant transformation involves the construction of an expression vectorwhich will function in plant cells. Such a vector comprises DNAcomprising a gene under control of, or operatively linked to, aregulatory element (for example, a promoter). The expression vector maycontain one or more such operably linked gene/regulatory elementcombinations. The vector(s) may be in the form of a plasmid, and can beused alone or in combination with other plasmids, to provide transformedcorn plants, using transformation methods as described below toincorporate transgenes into the genetic material of the corn plant(s).

Expression Vectors for Corn Transformation: Marker Genes

Expression vectors include at least one genetic marker, operably linkedto a regulatory element (a promoter, for example) that allowstransformed cells containing the marker to be either recovered bynegative selection, i.e., inhibiting growth of cells that do not containthe selectable marker gene, or by positive selection, i.e., screeningfor the product encoded by the genetic marker. Many commonly usedselectable marker genes for plant transformation are well known in thetransformation arts, and include, for example, genes that code forenzymes that metabolically detoxify a selective chemical agent which maybe an antibiotic or a herbicide, or genes that encode an altered targetwhich is insensitive to the inhibitor. A few positive selection methodsare also known in the art.

One commonly used selectable marker gene for plant transformation is theneomycin phosphotransferase II (nptII) gene, isolated from transposonTn5, which, when placed under the control of plant regulatory signals,confers resistance to kanamycin (Fraley et al., Proc. Natl. Acad. Sci.U.S.A., 80:4803 (1983)). Another commonly used selectable marker gene isthe hygromycin phosphotransferase gene which confers resistance to theantibiotic hygromycin (Vanden Elzen et al., Plant Mol. Biol., 5:299(1985)).

Additional selectable marker genes of bacterial origin that conferresistance to antibiotics include gentamycin acetyl transferase,streptomycin phosphotransferase, and aminoglycoside-3′-adenyltransferase, the bleomycin resistance determinant (Hayford et al., PlantPhysiol. 86:1216 (1988), Jones et al., Mol. Gen. Genet., 210:86 (1987),Svab et al., Plant Mol. Biol. 14:197 (1990), and Hille et al., PlantMol. Biol. 7:171 (1986)). Other selectable marker genes conferresistance to herbicides such as glyphosate, glufosinate or bromoxynil(Comai et al., Nature 317:741-744 (1985), Gordon-Kamm et al., Plant Cell2:603-618 (1990) and Stalker et al., Science 242:419-423 (1988)).

Selectable marker genes for plant transformation that are not ofbacterial origin include, for example, mouse dihydrofolate reductase,plant 5-enolpyruvylshikimate-3-phosphate synthase and plant acetolactatesynthase (Eichholtz et al., Somatic Cell Mol. Genet. 13:67 (1987), Shahet al., Science 233:478 (1986), and Charest et al., Plant Cell Rep.8:643 (1990)).

Another class of marker genes for plant transformation requiresscreening of presumptively transformed plant cells rather than directgenetic selection of transformed cells for resistance to a toxicsubstance such as an antibiotic. These genes are particularly useful toquantify or visualize the spatial pattern of expression of a gene inspecific tissues and are frequently referred to as reporter genesbecause they can be fused to a gene or gene regulatory sequence for theinvestigation of gene expression. Commonly used genes for screeningpresumptively transformed cells include beta-glucuronidase (GUS),beta-galactosidase, luciferase, and chloramphenicol acetyltransferase(Jefferson, R. A., Plant Mol. Biol. Rep. 5:387 (1987), Teeri et al.,EMBO J. 8:343 (1989), Koncz et al., Proc. Natl. Acad. Sci U.S.A. 84:131(1987), and DeBlock et al., EMBO J. 3:1681 (1984). Another approach tothe identification of relatively rare transformation events has been useof a gene that encodes a dominant constitutive regulator of the Zea maysanthocyanin pigmentation pathway (Ludwig et al., Science 247:449(1990)).

In vivo methods for visualizing GUS activity that do not requiredestruction of plant tissue are also available. However, these in vivomethods for visualizing GUS activity have not proven useful for recoveryof transformed cells because of low sensitivity, high fluorescentbackgrounds and limitations associated with the use of luciferase genesas selectable markers.

A gene encoding Green Fluorescent Protein (GFP) has been utilized as amarker for gene expression in prokaryotic and eukaryotic cells (Chalfieet al., Science 263:802 (1994)). GFP and mutants of GFP may be used asscreenable markers.

Expression Vectors for Corn Transformation: Promoters

Genes included in expression vectors must be driven by nucleotidesequence comprising a regulatory element, for example, a promoter.Several types of promoters are now well known in the transformationarts, as are other regulatory elements that can be used alone or incombination with promoters.

As used herein, “promoter” includes reference to a region of DNAupstream from the start of transcription and involved in recognition andbinding of RNA polymerase and other proteins to initiate transcription.A “plant promoter” is a promoter capable of initiating transcription inplant cells. Examples of promoters under developmental control includepromoters that preferentially initiate transcription in certain organs,such as leaves, roots, seeds and tissues such as fibers, xylem vessels,tracheids, or sclerenchyma. Such promoters are referred to as“tissue-preferred.” Promoters which initiate transcription only incertain tissue are referred to as “tissue-specific.” A “cell-type”specific promoter primarily drives expression in certain cell types inone or more organs, for example, vascular cells in roots or leaves. An“inducible” promoter is a promoter which is under environmental control.Examples of environmental conditions that may effect transcription byinducible promoters include anaerobic conditions or the presence oflight. Tissue-specific, tissue-preferred, cell-type specific, andinducible promoters constitute the class of “non-constitutive”promoters. A “constitutive” promoter is a promoter which is active undermost environmental conditions.

A. Inducible Promoters—An inducible promoter is operably linked to agene for expression in corn. Optionally, the inducible promoter isoperably linked to a nucleotide sequence encoding a signal sequencewhich is operably linked to a gene for expression in corn. With aninducible promoter the rate of transcription increases in response to aninducing agent. Any inducible promoter can be used in the instantinvention. See Ward et al., Plant Mol. Biol. 22:361-366 (1993).Exemplary inducible promoters include, but are not limited to, that fromthe ACEI system which responds to copper (Mett et al., Proc. Natl. Acad.Sci. U.S.A. 90:4567-4571 (1993)); In2 gene from maize which responds tobenzenesulfonamide herbicide safeners (Gatz et al., Mol. Gen. Genetics243:32-38 (1994)) or Tet repressor from Tn10 (Gatz et al., Mol. Gen.Genetics 227:229-237 (1991)). A particularly preferred induciblepromoter is a promoter that responds to an inducing agent to whichplants do not normally respond. An exemplary inducible promoter is theinducible promoter from a steroid hormone gene, the transcriptionalactivity of which is induced by a glucocorticosteroid hormone (Schena etal., Proc. Natl. Acad. Sci. U.S.A. 88:0421 (1991)).

B. Constitutive Promoters—A constitutive promoter is operably linked toa gene for expression in corn or the constitutive promoter is operablylinked to a nucleotide sequence encoding a signal sequence which isoperably linked to a gene for expression in corn. Many differentconstitutive promoters can be utilized in the instant invention.Exemplary constitutive promoters include, but are not limited to, thepromoters from plant viruses such as the 35S promoter from CaMV (Odellet al., Nature 313:810-812 (1985)) and the promoters from such genes asrice actin (McElroy et al., Plant Cell 2:163-171 (1990)); ubiquitin(Christensen et al., Plant Mol. Biol. 12:619-632 (1989) and Christensenet al., Plant Mol. Biol. 18:675-689 (1992)); pEMU (Last et al., Theor.Appl. Genet. 81:581-588 (1991)); MAS (Velten et al., EMBO J. 3:2723-2730(1984)) and maize H3 histone (Lepetit et al., Mol. Gen. Genetics231:276-285 (1992) and Atanassova et al., Plant Journal 2 (3): 291-300(1992)).

The ALS promoter, XbaI/NcoI fragment 5′ to the Brassica napus ALS3structural gene (or a nucleotide sequence similarity to said XbaI/NcoIfragment), represents a particularly useful constitutive promoter. SeePCT application WO96/30530.

C. Tissue-specific or Tissue-preferred Promoters—A tissue-specificpromoter is operably linked to a gene for expression in corn.Optionally, the tissue-specific promoter is operably linked to anucleotide sequence encoding a signal sequence which is operably linkedto a gene for expression in corn. Plants transformed with a gene ofinterest operably linked to a tissue-specific promoter produce theprotein product of the transgene exclusively, or preferentially, in aspecific tissue.

Any tissue-specific or tissue-preferred promoter can be utilized in theinstant invention. Exemplary tissue-specific or tissue-preferredpromoters include, but are not limited to, a root-preferred promoter,such as that from the phaseolin gene (Mural et al., Science 23:476-482(1983) and Sengupta-Gopalan et al., Proc. Natl. Acad. Sci. U.S.A.82:3320-3324 (1985)); a leaf-specific and light-induced promoter such asthat from cab or rubisco (Simpson et al., EMBO J. 4(11):2723-2729 (1985)and Timko et al., Nature 318:579-582 (1985)); an anther-specificpromoter such as that from LAT52 (Twell et al., Mol. Gen. Genetics217:240-245 (1989)); a pollen-specific promoter such as that from Zm13or a microspore-preferred promoter such as that from apg (Twell et al.,Sex. Plant Reprod. 6:217-224 (1993)).

Signal Sequences for Targeting Proteins to Subcellular Compartments

Transport of protein produced by transgenes to a subcellular compartmentsuch as the chloroplast, vacuole, peroxisome, glyoxysome, cell wall ormitochondrion or for secretion into the apoplast, is accomplished bymeans of operably linking the nucleotide sequence encoding a signalsequence to the 5′ and/or 3′ region of a gene encoding the protein ofinterest. Targeting sequences at the 5′ and/or 3′ end of the structuralgene may determine, during protein synthesis and processing, where theencoded protein is ultimately compartmentalized.

The presence of a signal sequence directs a polypeptide to either anintracellular organelle or subcellular compartment or for secretion tothe apoplast. Many signal sequences are known in the art. See, forexample Becker et al., Plant Mol. Biol. 20:49 (1992), Knox, C., et al.,Plant Mol. Biol. 9:3-17 (1987), Lerner et al., Plant Physiol. 91:124-129(1989), Fontes et al., Plant Cell 3:483-496 (1991), Matsuoka et al.,Proc. Natl. Acad. Sci. 88:834 (1991), Gould et al., J. Cell. Biol.108:1657 (1989), Creissen et al., Plant J. 2:129 (1991), Kalderon, etal., Cell 39:499-509 (1984), Stiefel, et al., Plant Cell 2:785-793(1990).

Foreign Protein Genes and Agronomic Genes

With transgenic plants according to the present invention, a foreignprotein can be produced in commercial quantities. Thus, techniques forthe selection and propagation of transformed plants, which are wellunderstood in the art, yield a plurality of transgenic plants which areharvested in a conventional manner, and a foreign protein then can beextracted from a tissue of interest or from total biomass. Proteinextraction from plant biomass can be accomplished by known methods whichare discussed, for example, by Heney and Orr, Anal. Biochem. 114:92-6(1981).

According to a preferred embodiment, the transgenic plant provided forcommercial production of foreign protein is corn. In another preferredembodiment, the biomass of interest is seed. For the relatively smallnumber of transgenic plants that show higher levels of expression, agenetic map can be generated, primarily via conventional RFLP, PCR andSSR analysis, which identifies the approximate chromosomal location ofthe integrated DNA molecule. For exemplary methodologies in this regard,see Glick and Thompson, Methods in Plant Molecular Biology andBiotechnology CRC Press, Boca Raton 269:284 (1993). Map informationconcerning chromosomal location is useful for proprietary protection ofa subject transgenic plant. If unauthorized propagation is undertakenand crosses made with other germplasm, the map of the integration regioncan be compared to similar maps for suspect plants, to determine if thelatter have a common parentage with the subject plant. Map comparisonswould involve hybridizations, RFLP, PCR, SSR and sequencing, all ofwhich are conventional techniques.

Likewise, by means of the present invention, agronomic genes can beexpressed in transformed plants. More particularly, plants can begenetically engineered to express various phenotypes of agronomicinterest. Exemplary genes implicated in this regard include, but are notlimited to, those categorized below:

1. Genes that Confer Resistance to Pests or Disease and that Encode:

A. Plant disease resistance genes. Plant defences are often activated byspecific interaction between the product of a disease resistance gene(R) in the plant and the product of a corresponding avirulence (Avr)gene in the pathogen. A plant inbred line can be transformed with clonedresistance gene to engineer plants that are resistant to specificpathogen strains. See, for example Jones et al., Science 266:789 (1994)(cloning of the tomato Cf-9 gene for resistance to Cladosporium fulvum);Martin et al., Science 262:1432 (1993) (tomato Pto gene for resistanceto Pseudomonas syringae pv. tomato encodes a protein kinase); Mindrinoset al., Cell 78:1089 (1994) (Arabidopsis RSP2 gene for resistance toPseudomonas syringae).

B. A Bacillus thuringiensis protein, a derivative thereof or a syntheticpolypeptide modeled thereon. See, for example, Geiser et al., Gene48:109 (1986), who disclose the cloning and nucleotide sequence of a Btalpha-endotoxin gene. Moreover, DNA molecules encoding alpha-endotoxingenes can be purchased from American Type Culture Collection, Manassas,Va., for example, under ATCC Accession Numbers 40098, 67136, 31995 and31998.

C. A lectin. See, for example, the article by Van Damme et al., PlantMolec. Biol. 24:25 (1994), who disclose the nucleotide sequences ofseveral Clivia miniata mannose-binding lectin genes.

D. A vitamin-binding protein such as avidin. See PCT application US93/06487. The application teaches the use of avidin and avidinhomologues as larvicides against insect pests.

E. An enzyme inhibitor, for example, a protease or proteinase inhibitoror an amylase inhibitor. See, for example, Abe et al., J. Biol. Chem.262:16793 (1987) (nucleotide sequence of rice cysteine proteinaseinhibitor), Huub et al., Plant Molec. Biol. 21:985 (1993) (nucleotidesequence of cDNA encoding tobacco proteinase inhibitor I), Sumitani etal., Biosci. Biotech. Biochem. 57:1243 (1993) (nucleotide sequence ofStreptomyces nitrosporeus alpha-amylase inhibitor).

F. An insect-specific hormone or pheromone such as an ecdysteroid orjuvenile hormone, a variant thereof, a mimetic based thereon, or anantagonist or agonist thereof. See, for example, the disclosure byHammock et al., Nature 344:458 (1990), of baculovirus expression ofcloned juvenile hormone esterase, an inactivator of juvenile hormone.

G. An insect-specific peptide or neuropeptide which, upon expression,disrupts the physiology of the affected pest. For example, see thedisclosures of Pratt et al., Biochem. Biophys. Res. Comm. 163:1243(1989) (an allostatin is identified in Diploptera puntata). See alsoU.S. Pat. No. 5,266,317 to Tomalski et al., who disclose genes encodinginsect-specific, paralytic neurotoxins.

H. An insect-specific venom produced in nature by a snake, a wasp, etc.For example, see Pang et al., Gene 116:165 (1992), for disclosure ofheterologous expression in plants of a gene coding for a scorpioninsectotoxic peptide.

I. An enzyme responsible for a hyper-accumulation of a monoterpene, asesquiterpene, a steroid, a hydroxamic acid, a phenylpropanoidderivative or another non-protein molecule with insecticidal activity.

J. An enzyme involved in the modification, including thepost-translational modification, of a biologically active molecule; forexample, a glycolytic enzyme, a proteolytic enzyme, a lipolytic enzyme,a nuclease, a cyclase, a transaminase, an esterase, a hydrolase, aphosphatase, a kinase, a phosphorylase, a polymerase, an elastase, achitinase and a glucanase, whether natural or synthetic. See PCTapplication WO 93/02197 in the name of Scott et al., which discloses thenucleotide sequence of a callase gene. DNA molecules which containchitinase-encoding sequences can be obtained, for example, from the ATCCunder Accession Numbers 39637 and 67152. See also Kramer et al., InsectBiochem. Molec. Biol. 23:691 (1993), who teach the nucleotide sequenceof a cDNA encoding tobacco hornworm chitinase, and Kawalleck et al.,Plant Molec. Biol. 21:673 (1993), who provide the nucleotide sequence ofthe parsley ubi4-2 polyubiquitin gene.

K. A molecule that stimulates signal transduction. For example, see thedisclosure by Botella et al., Plant Molec. Biol. 24:757 (1994), ofnucleotide sequences for mung bean calmodulin cDNA clones, and Griess etal., Plant Physiol. 104:1467 (1994), who provide the nucleotide sequenceof a maize calmodulin cDNA clone.

L. A hydrophobic moment peptide. See PCT application WO95/16776(disclosure of peptide derivatives of Tachyplesin which inhibit fungalplant pathogens) and PCT application WO 95/18855 (teaches syntheticantimicrobial peptides that confer disease resistance).

M. A membrane permease, a channel former or a channel blocker. Forexample, see the disclosure of Jaynes et al., Plant Sci 89:43 (1993), ofheterologous expression of a cecropin-beta, lytic peptide analog torender transgenic tobacco plants resistant to Pseudomonas solanacearum.

N. A viral-invasive protein or a complex toxin derived therefrom. Forexample, the accumulation of viral coat proteins in transformed plantcells imparts resistance to viral infection and/or disease developmenteffected by the virus from which the coat protein gene is derived, aswell as by related viruseS. See Beachy et al., Ann. Rev. Phytopathol.28:451 (1990). Coat protein-mediated resistance has been conferred upontransformed plants against alfalfa mosaic virus, cucumber mosaic virus,tobacco streak virus, potato virus X, potato virus Y, tobacco etchvirus, tobacco rattle virus and tobacco mosaic virus. Id.

O. An insect-specific antibody or an immunotoxin derived therefrom.Thus, an antibody targeted to a critical metabolic function in theinsect gut would inactivate an affected enzyme, killing the insect.

P. A virus-specific antibody. See, for example, Tavladoraki et al.,Nature 366:469 (1993), who show that transgenic plants expressingrecombinant antibody genes are protected from virus attack.

Q. A developmental-arrestive protein produced in nature by a pathogen ora parasite. Thus, fungal endo-alpha-1,4-D-polygalacturonases facilitatefungal colonization and plant nutrient release by solubilising plantcell wall homo-alpha-1,4-D-galacturonase. See Lamb et al., BioTechnology10:1436 (1992). The cloning and characterization of a gene which encodesa bean endopolygalacturonase-inhibiting protein is described by Toubartet al., Plant J. 2:367 (1992).

R. A developmental-arrestive protein produced in nature by a plant. Forexample, Logemann et al., BioTechnology 10:305 (1992), have shown thattransgenic plants expressing the barley ribosome-inactivating gene havean increased resistance to fungal disease.

2. Genes that Confer Resistance to an Herbicide, for Example:

A. An herbicide that inhibits the growing point or meristem, such as animidazolinone or a sulfonylurea. Exemplary genes in this category codefor mutant ALS and AHAS enzyme as described, for example, by Lee et al.,EMBO J. 7:1241 (1988), and Miki et al., Theor. Appl. Genet. 80:449(1990), respectively.

B. Glyphosate (resistance conferred by mutant5-enolpyruvylshikimate-3-phosphate synthase (EPSP) and aroA genes,respectively) and other phosphono compounds such as glufosinate(phosphinothricin acetyl transferase (PAT) and Streptomyceshygroscopicus (PAT bar genes), and pyridinoxy or phenoxy propionic acidsand cyclohexones (ACCase inhibitor-encoding genes). See, for example,U.S. Pat. No. 4,940,835 to Shah et al., which discloses the nucleotidesequence of a form of EPSP which can confer glyphosate resistance. A DNAmolecule encoding a mutant aroA gene can be obtained under ATCCaccession number 39256, and the nucleotide sequence of the mutant geneis disclosed in U.S. Pat. No. 4,769,061 to Comai. European patentapplication No. 0 333 033 to Kumada et al., and U.S. Pat. No. 4,975,374to Goodman et al., disclose nucleotide sequences of glutamine synthetasegenes which confer resistance to herbicides such as L-phosphinothricin.The nucleotide sequence of a PAT gene is provided in Europeanapplication No. 0 242 246 to Leemans et al. DeGreef et al.,BioTechnology 7:61 (1989), describe the production of transgenic plantsthat express chimeric bar genes coding for PAT activity. Exemplary ofgenes conferring resistance to phenoxy propionic acids and cyclohexones,such as sethoxydim and haloxyfop are the ABC5-S1, ABC5-S2 and ABC5-S3genes described by Marshall et al., Theor. Appl. Genet. 83:435 (1992).

C. An herbicide that inhibits photosynthesis, such as a triazine (psbAand gs+ genes) or a benzonitrile (nitrilase gene). Przibilla et al.,Plant Cell 3:169 (1991), describe the transformation of Chlamydomonaswith plasmids encoding mutant psbA genes. Nucleotide sequences fornitrilase genes are disclosed in U.S. Pat. No. 4,810,648 to Stalker, andDNA molecules containing these genes are available under ATCC AccessionNumbers 53435, 67441, and 67442. Cloning and expression of DNA codingfor a glutathione S-transferase is described by Hayes et al., Biochem.J. 285:173 (1992).

3. Genes that Confer or Contribute to a Value-Added Trait, Such as:

A. Modified fatty acid metabolism, for example, by transforming a plantwith an antisense gene of stearyl-ACP desaturase to increase stearicacid content of the plant. See Knutzon et al., Proc. Natl. Acad Sci.U.S.A. 89:2624 (1992)

B. Increased resistance to high light stress such as photo-oxidativedamages, for example by transforming a plant with a gene coding for aprotein of the Early Light Induced Protein family (BLIP) as described inWO 03/074713 in the name of Biogemma.

C. Modified carbohydrate composition effected, for example, bytransforming plants with a gene coding for an enzyme that alters thebranching pattern of starch. See Shiroza et al., J. Bact. 170:810 (1988)(nucleotide sequence of Streptococcus mutants fructosyltransferasegene), Steinmetz et al., Mol. Gen. Genet. 20:220 (1985) (nucleotidesequence of Bacillus subtilis levansucrase gene), Pen et al.,BioTechnology 10:292 (1992) (production of transgenic plants thatexpress Bacillus lichenifonnis α-amylase), Elliot et al., Plant Molec.Biol. 21:515 (1993) (nucleotide sequences of tomato invertase genes),Søgaard et al., J. Biol. Chem. 268:22480 (1993) (site-directedmutagenesis of barley alpha-amylase gene), and Fisher et al., PlantPhysiol. 102:1045 (1993) (maize endosperm starch branching enzyme II).

D. Increased resistance/tolerance to water stress or drought, forexample, by transforming a plant to create a plant having a modifiedcontent in ABA-Water-Stress-Ripening-Induced proteins (ARS proteins) asdescribed in WO 01/83753 in the name of Biogemma, or by transforming aplant with a nucleotide sequence coding for a phosphoenolpyruvatecarboxylase as shown in WO 02/081714. The tolerance of corn to droughtcan also be increased by an overexpression of phosphoenolpyruvatecarboxylase (PEPC-C4), obtained, for example from sorghum.

E. Increased content of cysteine and glutathione, useful in theregulation of sulfur compounds and plant resistance against variousstresses such as drought, heat or cold, by transforming a plant with agene coding for an Adenosine 5′ Phosphosulfate as shown in WO 01/49855.

F. Increased nutritional quality, for example, by introducing a zeingene which genetic sequence has been modified so that its proteinsequence has an increase in lysine and proline. The increasednutritional quality can also be attained by introducing into the maizeplant an albumin 2S gene from sunflower that has been modified by theaddition of the KDEL peptide sequence to keep and accumulate the albuminprotein in the endoplasmic reticulum.

G. Decreased phytate content: 1) Introduction of a phytase-encoding genewould enhance breakdown of phytate, adding more free phosphate to thetransformed plant. For example, see Van Hartingsveldt et al., Gene127:87 (1993), for a disclosure of the nucleotide sequence of anAspergillus niger phytase gene. 2) A gene could be introduced thatreduced phytate content. In maize, this, for example, could beaccomplished, by cloning and then reintroducing DNA associated with thesingle allele which is responsible for maize mutants characterized bylow levels of phytic acid. See Raboy et al., Maydica 35:383 (1990).

4. Genes that Control Male Sterility

A. Introduction of a deacetylase gene under the control of atapetum-specific promoter and with the application of the chemicalN-Ac-PPT. See international publication WO 01/29237.

B. Introduction of various stamen-specific promoters. See internationalpublications WO 92/13956 and WO 92/13957.

C. Introduction of the barnase and the barstar genes. See Paul et al.,Plant Mol. Biol. 19:611-622, 1992).

Examples of Transgenes

MON810, also known as MON810Bt or BT1, is the designation given by theMonsanto Company (St. Louis, Mo.) for the transgenic event that, whenexpressed in maize, produces an endotoxin that is efficacious againstthe European corn borer, Ostrinia nubilalis and certain otherLepidopteran larvae.

MON603, also known as MON603RR2, better known as NK603, is thedesignation for the transgenic event that, when expressed in maize,allows the use of glyphosate as a weed control agent on the crop.Another transgenic event, GA21, when expressed in maize, also allows theuse of glyphosate as a weed control agent on the crop.

MON89034, a designation given by the Monsanto Company (St. Louis, Mo.)for the transgenic event that, when expressed in maize, produces anendotoxin that is efficacious against the European corn borer, Ostrinianubilalis, fall armyworm, Spodoptera frugiperda, and certain otherLepidopteran larvae.

MON88017, also known as MON88017CCR1, is the transgenic event that, whenexpressed in maize, allows the use of glyphosate as a weed controlagent. In addition, this event produces an endotoxin that is efficaciousagainst the corn root worm, Diabrotica virgifera, and certain otherColeopteran larvae.

HERCULEX Corn Borer, better known as HX1 or TC1507, is the designationfor the transgenic event that, when expressed in maize, produces anendotoxin that is efficacious against the European corn borer, Ostrinianubilalis, and certain other Lepidopteran larvae. In addition, thetransgenic event was developed to allow the crop to be tolerant to theuse of glufosinate ammonium, the active ingredient in phosphinothricinherbicides.

HERCULEX Root Worm, or DAS59122-7, is the designation for the transgenicevent that, when expressed in maize, produces an endotoxin that isefficacious against the corn root worm, Diabrotica virgifera, andcertain other Coleopteran larvae. In addition, the transgenic event wasdeveloped to allow the crop to be tolerant to the use of glufosinateammonium, the active ingredient in phosphinothricin herbicides.

T25 is the designation for the transgenic event that, when expressed inmaize, allows the crop to be tolerant to the use of glufosinateammonium, the active ingredient in phosphinothricin herbicides.

Methods for Corn Transformation

Numerous methods for plant transformation have been developed, includingbiological and physical plant transformation protocols. See, forexample, Miki et al., “Procedures for Introducing Foreign DNA intoPlants” in Methods in Plant Molecular Biology and Biotechnology, GlickB. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993) pages67-88. In addition, expression vectors and in vitro culture methods forplant cell or tissue transformation and regeneration of plants areavailable. See, for example, Gruber et al., “Vectors for PlantTransformation” in Methods in Plant Molecular Biology and Biotechnology,Glick B. R. and Thompson, J. E. Eds. (CRC Press, Inc., Boca Raton, 1993)pages 89-119.

A. Agrobacterium-mediated Transformation—One method for introducing anexpression vector into plants is based on the natural transformationsystem of Agrobacterium. See, for example, Horsch et al., Science227:1229 (1985). A. tumefaciens and A. rhizogenes are plant pathogenicsoil bacteria which genetically transform plant cells. The Ti and Riplasmids of A. tumefaciens and A. rhizogenes, respectively, carry genesresponsible for genetic transformation of the plant. See, for example,Kado, C. I., Crit. Rev. Plant Sci. 10:1 (1991). Descriptions ofAgrobacterium vector systems and methods for Agrobacterium-mediated genetransfer are provided by Gruber et al., supra, Miki et al., supra, andMoloney et al., Plant Cell Reports 8:238 (1989). See also, U.S. Pat. No.5,591,616 issued Jan. 7, 1997.

B. Direct Gene Transfer—Despite the fact the host range forAgrobacterium-mediated transformation is broad, some major cereal cropspecies and gymnosperms have generally been recalcitrant to this mode ofgene transfer, even though some success has recently been achieved inrice and corn. Hiei et al., The Plant Journal 6:271-282 (1994) and U.S.Pat. No. 5,591,616 issued Jan. 7, 1997. Several methods of planttransformation, collectively referred to as direct gene transfer, havebeen developed as an alternative to Agrobacterium-mediatedtransformation.

A generally applicable method of plant transformation ismicroprojectile-mediated transformation wherein DNA is carried on thesurface of microprojectiles measuring 1 to 4 micron. The expressionvector is introduced into plant tissues with a biolistic device thataccelerates the microprojectiles to speeds of 300 to 600 m/s which issufficient to penetrate plant cell walls and membranes. Sanford et al.,Part. Sci. Technol. 5:27 (1987), Sanford, J. C., Trends Biotech. 6:299(1988), Klein et al., BioTechnology 6:559-563 (1988), Sanford, J. C.,Physiol Plant 7:206 (1990), Klein et al., BioTechnology 10:268 (1992).In corn, several target tissues can be bombarded with DNA-coatedmicroprojectiles in order to produce transgenic plants, including, forexample, callus (Type I or Type II), immature embryos, and meristematictissue.

Another method for physical delivery of DNA to plants is sonication oftarget cells. Zhang et al., BioTechnology 9:996 (1991). Alternatively,liposome and spheroplast fusion have been used to introduce expressionvectors into plants. Deshayes et al., EMBO J., 4:2731 (1985), Christouet al., Proc Natl. Acad. Sci. U.S.A. 84:3962 (1987). Direct uptake ofDNA into protoplasts using CaCl₂ precipitation, polyvinyl alcohol orpoly-L-ornithine has also been reported. Hain et al., Mol. Gen. Genet.199:161 (1985) and Draper et al., Plant Cell Physiol. 23:451 (1982).Electroporation of protoplasts and whole cells and tissues have alsobeen described. D'Halluin et al., Plant Cell 4:1495-1505 (1992) andSpencer et al., Plant Mol. Biol. 24:51-61 (1994).

Following transformation of corn target tissues, expression of theabove-described selectable marker genes allows for preferentialselection of transformed cells, tissues and/or plants, usingregeneration and selection methods now well known in the art.

The foregoing methods for transformation would typically be used forproducing a transgenic inbred line. The transgenic inbred line couldthen be crossed, with another (non-transformed or transformed) inbredline, in order to produce a new transgenic inbred line. Alternatively, agenetic trait which has been engineered into a particular corn lineusing the foregoing transformation techniques could be moved intoanother line using traditional backcrossing techniques that are wellknown in the plant breeding arts. For example, a backcrossing approachcould be used to move an engineered trait from a public, non-eliteinbred line into an elite inbred line, or from an inbred line containinga foreign gene in its genome into an inbred line or lines which do notcontain that gene. As used herein, “crossing” can refer to a simple X byY cross, or the process of backcrossing, depending on the context.

When the term inbred corn plant is used in the context of the presentinvention, this also includes any inbred corn plant where one or moredesired traits have been introduced through backcrossing methods,whether such trait is a naturally occurring one or a transgenic one.Backcrossing methods can be used with the present invention to improveor introduce one or more characteristic into the inbred. The termbackcrossing as used herein refers to the repeated crossing of a hybridprogeny back to one of the parental corn plants for that inbred. Theparental corn plant which contributes the gene or the genes for thedesired characteristic is termed the nonrecurrent or donor parent. Thisterminology refers to the fact that the nonrecurrent parent is used onetime in the backcross protocol and therefore does not recur. Theparental corn plant to which the gene or genes from the nonrecurrentparent are transferred is known as the recurrent parent as it is usedfor several rounds in the backcrossing protocol (Fehr, 1987).

In a typical backcross protocol, the original inbred of interest(recurrent parent) is crossed to a second inbred (nonrecurrent parent)that carries the gene or genes of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a corn plant isobtained wherein all the desired morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant in addition to the gene or genes transferred from the nonrecurrentparent. It should be noted that some, one, two, three or more,self-pollination and growing of a population might be included betweentwo successive backcrosses. Indeed, an appropriate selection in thepopulation produced by the self-pollination, i.e., selection for thedesired trait and physiological and morphological characteristics of therecurrent parent might be equivalent to one, two or even threeadditional backcrosses in a continuous series without rigorousselection, saving time, money and effort for the breeder. The backcrossprocess could also be accelerated through a step of haploid inductiontogether by a molecular marker screening to identify the backcrossprogeny plants that have the closest genetic resemblance with therecurrent line, together with the gene or genes of interest to betransferred. A non limiting example of such a protocol would be thefollowing: a) the first generation F₁ produced by the cross of therecurrent parent A by the donor parent B is backcrossed to parent A, b)selection is practiced for the plants having the desired trait of parentB, c) selected plants are self-pollinated to produce a population ofplants where selection is practiced for the plants having the desiredtrait of parent B and the physiological and morphologicalcharacteristics of parent A, d) the selected plants are backcrossed one,two, three, four, five or more times to parent A to produce selectedbackcross progeny plants comprising the desired trait of parent B andthe physiological and morphological characteristics of parent A. Step c)may or may not be repeated and included between the backcrosses of stepd. Step c) may or may not be followed by a step of haploid inductionfollowed by molecular marker screening.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute one or more trait(s) or characteristic(s) in theoriginal inbred. To accomplish this, a gene or genes of the recurrentinbred is modified or substituted with the desired gene or genes fromthe nonrecurrent parent, while retaining essentially all of the rest ofthe desired genetic, and therefore the desired physiological andmorphological, constitution of the original inbred. The choice of theparticular nonrecurrent parent will depend on the purpose of thebackcross; one of the major purposes is to add some commerciallydesirable, agronomically important trait(s) to the plant. The exactbackcrossing protocol will depend on the characteristic(s) or trait(s)being altered to determine an appropriate testing protocol. Althoughbackcrossing methods are simplified when the characteristic beingtransferred is a single gene and dominant allele, multiple genes andrecessive allele(s) may also be transferred and therefore, backcrossbreeding is by no means restricted to character(s) governed by one or afew genes. In fact the number of genes might be less important than theidentification of the character(s) in the segregating population. Inthis instance it may then be necessary to introduce a test of theprogeny to determine if the desired characteristic(s) has beensuccessfully transferred. Such tests encompass not only visualinspection and simple crossing, but also follow up of thecharacteristic(s) through genetically associated markers and molecularassisted breeding tools. For example, selection of progeny containingthe transferred trait is done by direct selection, visual inspection fora trait associated with a dominant allele, while the selection ofprogeny for a trait that is transferred via a recessive allele, such asthe waxy starch characteristic, require selfing the progeny to determinewhich plant carry the recessive allele(s).

Many single gene traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single gene traits may or may not betransgenic, i.e., they may be naturally present in the nonrecurrentparent, examples of these traits include but are not limited to, malesterility, waxy starch, amylose starch, herbicide resistance, resistancefor bacterial, fungal, or viral disease, insect resistance, malefertility, water stress tolerance, enhanced nutritional quality,industrial usage, increased digestibility, yield stability and yieldenhancement. An example of this is the Rp1D gene which controlsresistance to rust fungus by preventing P. sorghi from producing spores.The Rp1D gene is usually preferred over the other Rp genes because it iswidely effective against all races of rust, but the emergence of newraces has lead to the use of other Rp genes comprising, for example, theRp1E, Rp1G, Rp1I, Rp1K or “compound” genes which combine two or more Rpgenes including Rp1GI, Rp1GDJ, etc. These genes are generally inheritedthrough the nucleus. Some known exceptions to this are the genes formale sterility, some of which are inherited cytoplasmically, but stillact as single gene traits. Several of these single gene traits aredescribed in U.S. Pat. Nos. 5,777,196; 5,948,957 and 5,969,212, thedisclosures of which are specifically hereby incorporated by reference.Genes related to digestibility are known to one skilled in the art, suchas those described in U.S. Pat. Nos. 8,20,302, 8,143,482 and 8,088,95.Each of the references mentioned above is herein incorporated intoreference by its entirety.

In 1981, the backcross method of breeding accounted for 17% of the totalbreeding effort for inbred line development in the United States,according to, Hallauer, A. R. et al., (1988) “Corn Breeding” in Corn andCorn Improvement, No. 18, pp. 463-481. The backcross breeding methodprovides a precise way of improving varieties that excel in a largenumber of attributes but are deficient in a few characteristics (Allard,1960, Principles of Plant Breeding, John Wiley & Sons, Inc.). The methodmakes use of a series of backcrosses to the variety to be improvedduring which the character or the characters in which improvement issought is maintained by selection. At the end of the backcrossing, thegene or genes being transferred unlike all other genes will beheterozygous. Selfing after the last backcross produces homozygosity forthis gene pair(s) and, coupled with selection, will result in a varietywith exactly the adaptation, yielding ability and qualitycharacteristics of the recurrent parent but superior to that parent inthe particular characteristic(s) for which the improvement program wasundertaken. Therefore, this method provides the plant breeder with ahigh degree of genetic control of his work.

Backcrossing is a powerful mechanism for achieving homozygosity and anypopulation obtained by backcrossing may rapidly converge on the genotypeof the recurrent parent. When backcrossing is made the basis of a plantbreeding program, the genotype of the recurrent parent will be modifiedwith regards to genes being transferred, which are maintained in thepopulation by selection.

Examples of successful backcrosses are the transfer of stem rustresistance from “Hope” wheat to “Bart” wheat and the transfer of buntresistance to “Bart” wheat to create “Bart 38” which has resistance toboth stem rust and bunt. Also highlighted by Allard is the successfultransfer of mildew, leaf spot and wilt resistances in “CaliforniaCommon” alfalfa to create “Caliverde.” This new “Caliverde” varietyproduced through the backcross process is indistinguishable from“California Common” except for its resistance to the three nameddiseases.

One of the advantages of the backcross method is that the breedingprogram can be carried out in almost every environment that will allowthe development of the character being transferred. Another advantage ofthe backcross method is that more than one character or trait can betransferred, either through several backcrosses or through the use oftransformation and then backcrossing.

The backcross technique is not only desirable when breeding for diseaseresistance but also for the adjustment of morphological characters,color characteristics and simply inherited quantitative characters suchas earliness, plant height and seed size and shape. In this regard, amedium grain type variety, “Calady,” has been produced by Jones andDavis. In dealing with quantitative characteristics, they selected thedonor parent with the view of sacrificing some of the intensity of thecharacter for which it was chosen, i.e., grain size. “Lady Wright,” along grain variety was used as the donor parent and “Coloro,” a shortgrain one as the recurrent parent. After four backcrosses, the mediumgrain type variety “Calady” was produced.

Deposit Information

A deposit of the inbred corn line of this invention is maintained byAgReliant Genetics, LLC, 4640 East SR32, Lebanon, Ind. 46052. AgReliantmaintains the seed deposit on behalf of Limagrain Europe and KWS SAATAG. In addition, a sample of the inbred corn seed of this invention hasbeen or will be deposited with the American Type Culture Collection,10801 University Boulevard, Manassas, Va. 20110 or the NationalCollections of Industrial, Food and Marine Bacteria (NCIMB), 23 StMachar Drive, Aberdeen, Scotland, AB24 3RY, United Kingdom.

To satisfy the enablement requirements of 35 U.S.C. 112, and to certifythat the deposit of the isolated strain (i.e., corn inbred) of thepresent invention meets the criteria set forth in 37 CFR 1.801-1.809 andManual of Patent Examining Procedure (MPEP) 2402-2411.05, Applicantshereby make the following statements regarding the deposited corn inbredline II15 (deposited as ATCC Accession No. 123784):

If the deposit is made under the terms of the Budapest Treaty, theinstant invention will be irrevocably and without restriction releasedto the public upon the granting of a patent.

If the deposit is made not under the terms of the Budapest Treaty,Applicant(s) provides assurance of compliance by following statements:

-   -   1. During the pendency of this application, access to the        invention will be afforded to the Commissioner upon request;    -   2. All restrictions on availability to the public will be        irrevocably removed upon granting of the patent under conditions        specified in 37 CFR 1.808;    -   3. The deposit will be maintained in a public repository for a        period of 30 years or 5 years after the last request or for the        effective life of the patent, whichever is longer;    -   4. A test of the viability of the biological material at the        time of deposit will be conducted by the public depository under        37 CFR 1.807; and    -   5. The deposit will be replaced if it should ever become        unavailable.

Access to this deposit will be available during the pendency of thisapplication to persons determined by the Commissioner of Patents andTrademarks to be entitled thereto under 37 C.F.R. §1.14 and 35 U.S.C.§122. Upon granting of any claims in this application, all restrictionson the availability to the public of the variety will be irrevocablyremoved by affording access to a deposit of at least 2,500 seeds of thesame variety with the ATCC or NCIMB.

INDUSTRIAL APPLICABILITY

Corn is used as human food, livestock feed, and as raw material inindustry. The food uses of corn, in addition to human consumption ofcorn kernels, include both products of dry- and wet-milling industries.The principal products of corn dry-milling are grits, meal and flour.Corn meal is flour ground to fine, medium, and coarse consistencies fromdried corn. In the United States, the finely ground corn meal is alsoreferred to as corn flour. However, the term “corn flour” denotes cornstarch in the United Kingdom. Corn meal has a long shelf life and isused to produce an assortment of products, including but not limited totortillas, taco shells, bread, cereal and muffins.

The corn wet-milling industry can provide corn starch, corn syrups, cornsweeteners and dextrose for food use. Corn syrup is used in foods tosoften texture, add volume, prevent crystallization of sugar and enhanceflavor. Corn syrup is distinct from high-fructose corn syrup (HFCS),which is created when corn syrup undergoes enzymatic processing,producing a sweeter compound that contains higher levels of fructose.

Corn oil is recovered from corn germ, which is a by-product of both dry-and wet-milling industries. Corn oil which is high in mono and polyunsaturated fats, is used for reducing fat and trans fat in numerousfood products.

Corn, including both grain and non-grain portions of the plant, is alsoused extensively as livestock feed, primarily for beef cattle, dairycattle, hogs and poultry.

Industrial uses of corn include production of ethanol, corn starch inthe wet-milling industry and corn flour in the dry-milling industry.Corn ethanol is ethanol produced from corn as a biomass throughindustrial fermentation, chemical processing and distillation. Corn isthe main feedstock used for producing ethanol fuel in the United States.The industrial applications of corn starch and flour are based onfunctional properties, such as viscosity, film formation, adhesiveproperties, and ability to suspend particles. Corn starch and flour alsohave application in the paper and textile industries. Other industrialuses include applications in adhesives, building materials, foundrybinders, laundry starches, explosives, oil-well muds and other miningapplications.

Plant parts other than the grain of corn are also used in industry, forexample, stalks and husks are made into paper and wallboard and cobs areused for fuel and to make charcoal.

The seed of inbred corn lines BB208, BB209, BB210, BB211, BC146, BC147,CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65, the plant producedfrom the inbred seed, the hybrid corn plant produced from the crossingof the inbred, hybrid seed, and various parts of the hybrid corn plantand transgenic versions of the foregoing, can be utilized for humanfood, livestock feed, and as a raw material in industry.

Tables of Field Test Trials

In the tables that follow, exemplary traits and characteristics ofhybrid combinations having inbred corn lines BB208, BB209, BB210, BB211,BC146, BC147, CB21, CB34, CB39, II15, II17, IM5, LK1, and MM65 as aparental line are given compared to other hybrids. The data collectedare presented for key characteristics and traits. The field tests areexperimental trials and have been made at numerous locations, with oneor two replications per location under supervision of the applicant.Information about these experimental hybrids as compared to the checkhybrids is presented.

Field Test Trials:

Information for each pedigree includes:

-   -   1. Mean yield of the hybrid (YLD) across all locations        (bushels/acre) is shown in column 3.    -   2. A mean for the percentage moisture (Mst %) for the hybrid        across all locations is shown in column 4    -   3. A mean of the yield divided by the percentage moisture (Y/M)        for the hybrid across all locations is shown in column 5.    -   4. Test weight (TWgt) is the grain density as measured in pounds        per bushel and is shown in column 6    -   5. A mean of the percentage of plants with stalk lodging (SL %)        across all locations is shown in column 7.    -   6. A mean of the percentage of plants with root lodging (RL %)        across all locations is shown in column 8.    -   7. Ear Height (EHT) is a physical measurement taken from the        ground level to the node of attachment for the upper ear. It is        expressed to the nearest tenth of a foot and is shown in column        9.    -   8. Plant Height (PLHT) is a physical measurement taken from the        ground level to the tip of the tassel. It is expressed to the        nearest tenth of a foot and is shown in column 10.    -   9. Harvest Appearance (HA n) is a rating made by a trained        person on the date of harvest. Harvest appearance is the rater's        impression of the hybrid based on, but not limited to, a        combination of factors to include plant intactness, tissue        health appearance and ease of harvest as it relates to stalk        lodging and root lodging. A scale of 1=Lowest to 9=Highest/Most        Desirable is used and is listed in column 11.        BB208

TABLE 2 Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA nComparative Data for 212US01109, a Hybrid Having BB208 as One InbredParent Overall comparisons: Year 1 field trials, 12 reps 210UM01241 +603 HCL112RR2 × HCL437 162.8 16.0 10.2 56.6 0.6 3.1 3.3 8.9 4.09DKC52-59 9DKC52-59 169.7 16.2 10.5 54.9 8.9 1.6 3.8 8.9 4.1 212US01109BB208 × ML12 180.1 18.0 10.0 55.2 −0.1 10.8 4.1 10.3 4.6 9P0463XR9P0463XR 173.9 18.5 9.4 57.0 0.2 3.0 3.6 9.0 6.0 208UE01323 + NZRHCL301RR2 × HCL516BT1 175.8 19.0 9.3 55.7 5.8 11.6 3.3 8.9 4.0210UA02090 + VTU R6258RMQKZ × ML8 186.2 19.1 9.7 55.0 5.2 4.2 3.9 9.75.7 206UR01913 + G21 NP2660GT × NP2727 162.3 19.1 8.5 55.7 0.7 15.1 3.910.0 5.6 Comparative Data for 212US01103, a Hybrid Having BB208 as OneInbred Parent Overall comparisons: Year 1 field trials, 19 reps212US01103 BB208 × ML8 173.8 17.2 10.1 54.9 4.9 1.4 3.6 8.8 5.19DKC52-59 9DKC52-59 175.5 18.1 9.7 56.2 4.3 0.4 3.5 8.1 4.8 210UR01119 +GSS R6258RMQKZ × R2683LMSLZ 165.2 19.5 8.5 56.9 3.3 1.8 3.8 9.0 5.8206UR01913 + G21 NP2660GT × NP2727 162.9 19.7 8.3 56.4 2.7 5.8 3.7 8.96.0 208UE01323 + NZR HCL301RR2 × HCL516BT1 164.0 20.1 8.2 56.5 5.0 4.03.4 8.3 4.8 Comparative Data for 212US01104 and 212US01110, HybridsHaving BB208 as One Inbred Parent Overall comparisons: Year 2 fieldtrials, 23 reps 212US01104 BB208 × ML9 185.2 15.3 12.1 59.1 1.1 4.6 3.08.1 5.0 212UE01938 + VTU R9540RMQKZ × HCL5015 173.4 15.4 11.3 60.1 1.63.9 2.9 8.2 5.2 212US01110 BB208 × MM53 191.8 15.9 12.1 58.8 2.7 5.3 3.18.3 4.9 9DKC53-78 9DKC53-78 176.9 16.0 11.1 59.4 1.8 0.0 2.9 7.8 5.29P0115AM1 9P0115AM1 175.4 16.3 10.8 59.1 0.3 9.4 3.1 8.0 5.2210UA02168 + 603 SGI064RHTTZ × SGI065 180.9 16.9 10.7 58.2 10.4 0.2 3.27.8 4.3 212UC00914 + VTU RBO1RMQKZ × MM53 197.3 17.3 11.4 56.3 1.0 5.63.4 9.2 5.8 210US02194 + VTU F4780RMQKZ × T5972Z 187.3 17.7 10.6 58.13.0 4.0 3.1 8.4 5.5 210UA02091 + AG4 NP2660GTCBLLRW × NP2727 183.1 17.910.2 56.5 0.3 7.3 3.6 9.2 6.3 210UA02090 + VTU R6258RMQKZ × ML8 178.819.3 9.3 55.7 0.8 3.6 3.5 8.5 5.8 Comparative Data for 212US02336 + VTU,212US02337 + VTU, and 212US02338 + VTU, Hybrids Having BB208 as OneInbred Parent Overall comparisons: Year 2 field trials, 9 reps210UA02079 + YGC R2999RBDHZ × HCL4003BT1 161.5 14.8 10.9 61.3 0.5 3.84.4 212US02338 + VTU BB208RMQKZ × MM53 191.3 15.7 12.2 60.3 0.0 2.0 4.7210UR01118 + VTU HCL112CCR1 × A5338ZNYKZ 166.7 15.7 10.6 60.7 0.0 0.04.7 212UE01938 + VTU R954ORMQKZ × HCL5015 172.6 16.0 10.8 60.1 0.0 0.04.9 212US02336 + VTU BB208RMQKZ × ML8 185.4 16.5 11.2 59.2 0.2 1.3 4.7210US02194 + VTU F478ORMQKZ × T5972Z 186.4 18.2 10.2 58.3 0.6 3.5 5.9212US02337 + VTU BB208RMQKZ × ML12 192.1 19.5 9.9 56.7 0.5 1.4 5.3210UA02090 + VTU R6258RMQKZ × ML8 183.1 20.0 9.2 57.0 0.1 1.7 6.0Comparative Data for 212US02338 + VTU, a Hybrid Having BB208 as OneInbred Parent Overall comparisons: Year 3 field trials, 10 reps212US02338 + VTU BB208RMQKZ × MM53 215.9 17.5 12.3 53.5 0.1 0.3 4.0 9.75.6 212UC00914 + VTU RBO1RMQKZ × MM53 212.2 17.7 12.0 54.0 0.0 0.0 4.29.8 5.7 213UR02175 + GSS R2999GJLHZ × R3414LFWMZ 194.8 17.8 10.9 55.80.0 0.0 4.1 9.0 6.3 211UC01094 + 017 CB18 × MN7RMQKZ 214.5 18.4 11.752.4 0.1 0.0 4.3 9.8 6.2 Comparative Data for 212US01110, a HybridHaving BB208 as One Inbred Parent Overall comparisons: Year 3 fieldtrials, 24 reps 212US01110 BB208 × MM53 197.2 19.7 10.0 52.9 1.4 3.0 4.09.9 5.3 213UM02224 + GSS R2999RMQKB × T5842LMSLZ 198.9 20.3 9.8 54.3 0.60.1 3.6 9.0 5.9 212UC00890 + VTU RBO1RMQKZ × ML9 191.7 20.5 9.4 53.6 0.10.1 3.8 9.5 6.1 9DKC53-56RIB 9DKC53-56RIB 199.4 21.1 9.5 54.6 0.0 0.04.1 9.4 6.3 212UC00914 + VTU RBO1RMQKZ × MM53 194.5 21.1 9.2 52.8 0.01.5 3.8 9.1 5.7 9P0533AM1 9P0533AM1 202.2 21.6 9.4 55.2 0.1 0.7 3.5 9.15.3 211UA02203 + VTU MEF2526RMQKZ × HCL4029 191.2 21.8 8.8 54.9 0.0 0.53.5 9.1 5.6 Comparative Data for 212US02336 + VTU, 212US02337 + VTU, and212US02338 + VTU, Hybrids Having BB208 as One Inbred Parent Overallcomparisons: Year 3 field trials, 16 reps 212US02336 + VTU BB208RMQKZ ×ML8 194.6 17.0 11.4 52.2 1.5 0.3 4.3 10.3 5.6 212US02338 + VTUBB208RMQKZ × MM53 208.7 19.3 10.8 53.1 0.5 1.8 4.0 10.6 5.1 212UC00914 +VTU RBO1RMQKZ × MM53 194.7 19.9 9.8 53.1 0.0 1.9 4.3 11.2 5.9212US02337 + VTU BB208RMQKZ × ML12 203.0 20.0 10.2 53.3 0.6 4.2 4.3 10.35.9 213UM02224 + GSS R2999RMQKB × T5842LMSLZ 193.7 20.1 9.6 54.8 0.4 3.04.6 10.2 6.3 9DKC53-56RIB 9DKC53-56RIB 194.9 20.2 9.6 55.0 0.0 0.2 3.910.2 6.0 212UC00890 + VTU RBO1RMQKZ × ML9 196.3 20.5 9.6 53.7 0.0 5.64.1 10.9 6.2 211UA02203 + VTU MEF2526RMQKZ × HCL4029 188.9 20.5 9.2 56.20.0 0.6 4.4 10.2 5.6 9P0533AM1 9P0533AM1 195.9 21.5 9.1 55.0 0.6 1.3 4.09.9 5.4 Comparative Data for 213US01094 + VTU, 213US01160 + VTU, and213UT01267 + VTU, Hybrids Having BB208 as One Inbred Parent Overallcomparisons: Year 3 field trials, 23 reps 213US01094 + VTU BB208RMQKZ ×ML9 203.7 18.6 11.0 52.7 0.0 0.0 3.4 9.0 5.8 213US01160 + VTU BB208RMQKZ× MM70 198.7 18.7 10.6 51.9 2.5 0.0 3.5 9.1 4.9 213UT01267 + VTUBB208RMQKB × NL6 199.6 19.4 10.3 52.6 2.0 0.3 3.9 9.6 5.8 9DKC53-56RIB9DKC53-56R1B 198.1 20.4 9.7 54.8 0.0 0.0 3.2 8.9 5.8 213UM02224 + GSSR2999RMQKB × T5842LMSLZ 197.5 20.4 9.7 54.1 0.5 0.7 3.4 8.9 5.9212UC00890 + VTU RBO1RMQKZ × ML9 191.0 20.4 9.4 53.4 0.8 1.0 3.4 9.2 6.0211UA02203 + VTU MEF2526RMQKZ × HCL4029 189.7 20.4 9.3 56.0 0.1 −0.1 3.28.3 6.2 212UC00914 + VTU RBO1RMQKZ × MM53 201.8 20.6 9.8 52.4 0.0 2.83.6 9.7 5.7 9P0533AM1 9P0533AM1 200.7 21.5 9.3 55.2 0.0 0.3 3.1 8.8 5.7Comparative Data for 213US01094 + VTU, 213US01152 + VTU, 214US01523 +VTU, and 214UV02565 + VTU, Hybrids Having BB208 as One Inbred ParentOverall comparisons: Year 4 field trials, 24 reps 213US01094 + VTUBB208RMQKZ × ML9 197.9 16.4 12.1 52.9 0.8 4.3 3.7 9.4 5.6 214UV02565 +VTU BB208RMQKZ × KL6 198.8 16.9 11.8 51.6 3.9 0.3 4.0 10.1 5.0213US01152 + VTU BB208RMQKZ × MM69 192.9 17.4 11.1 52.2 2.9 2.1 4.1 10.04.9 212UC00914 + VTU RBO1RMQKZ × MM53 195.3 18.6 10.5 52.8 1.0 4.8 4.210.5 4.9 214US01523 + VTU BB208 × MM53RMQKZ 205.0 19.6 10.5 53.2 3.1 4.44.0 10.0 5.4 213UM02224 + GSS R2999RMQKB × T5842LMSLZ 196.3 19.7 10.054.4 2.4 0.8 3.7 9.9 5.4 9DKC53-56R1B 9DKC53-56R1B 200.7 19.8 10.1 54.50.5 1.2 3.5 9.2 5.3 213UA02356 + GSS A1601GJLHZ × A5105LFWMZ 198.2 20.59.7 55.1 0.1 −0.1 4.1 9.8 5.0 212UE02102 + GSS F0297RMQKZ × F1266LMSLZ199.8 21.4 9.3 54.2 1.0 5.8 3.9 9.6 4.6 Comparative Data for213US01144 + VTU, and 214UV02564 + VTU, Hybrids Having BB208 as OneInbred Parent Overall comparisons: Year 4 field trials, 58 reps214UV02564 + VTU BB208RMQKZ × MM68 196.1 16.3 12.0 52.2 1.6 0.2 3.5 9.44.3 213US01144 + VTU BB208RMQKZ × MM67 194.3 18.1 10.7 51.6 3.5 1.0 3.59.3 5.5 212UC00914 + VTU RBO1RMQKZ × MM53 194.5 18.3 10.6 53.3 0.8 1.23.7 9.5 5.1 213UA02356 + GSS A1601GJLHZ × A5105LFWMZ 193.7 19.4 10.055.2 0.4 0.0 3.7 9.3 5.1 9DKC53-56RIB 9DKC53-56RIB 193.3 19.4 10.0 54.70.2 2.6 3.3 9.0 5.2 213UM02224 + GSS R2999RMQKB × T5842LMSLZ 198.3 19.610.1 54.6 0.6 0.6 3.4 9.2 5.2 9P0533AM1 9P0533AM1 199.7 21.0 9.5 55.60.8 2.6 3.1 8.6 4.7 212UE02102 + GSS F0297RMQKZ × F1266LMSLZ 195.9 21.09.3 54.6 0.6 1.5 3.4 9.1 4.8BB209

TABLE 3 Yld Hybrid Grain H2O Y/M SL % RL % TW Asp PlHt EHt Overallcomparisons: First Year field trials, 5 reps BB59 × LH287BtCCR 208.423.0 9.2 0.0 6.8 56.8 7.3 280.0 100.0 BB209 × ML9 199.3 18.7 10.8 0.00.9 58.0 6.3 260.0 90.0 BB38 × ML9 191.2 20.1 9.6 0.0 0.4 58.4 7.1 260.0110.0 BC110 × ML9 171.9 18.5 9.3 0.0 1.5 58.4 7.1 300.0 90.0 Overallcomparisons: Second Year field trials, 11 reps BB209 × MN7RMQKZ 191.319.9 10.3 0.7 0.2 51.7 6.2 277.4 94.5 CB18 × MN7RMQKZ 186.5 19.8 10.10.2 1.1 51.9 6.5 259.1 94.5 BB59 × A1555RMQKZ 181.7 22.0 8.9 0.2 5.450.9 7.1 274.3 91.4 P1018AM1 180.0 20.9 9.1 0.3 5.8 54.3 7.0 295.7 109.7DKC57-50 176.8 19.2 9.7 0.0 0.0 56.9 5.8 274.3 85.3 Overall comparisons:Third Year field trials, 24 reps A1555RMQKZ × BB209 216.1 22.7 10.0 0.42.4 51.8 6.3 298.7 121.9 BB59 × A1555RMQKZ 212.2 21.8 10.0 0.0 1.2 51.96.8 280.4 112.8 CB18 × MN7RMQKZ 210.9 20.0 11.0 0.0 0.0 51.9 6.0 283.5112.8 P0832AMX 207.1 21.4 10.0 0.1 5.8 55.6 5.8 289.6 131.1 DKC57-75R1B205.7 21.8 10.0 0.0 2.2 54.3 6.6 268.2 106.7 BB46 × MM27RMQKZ 204.8 21.210.0 0.0 3.1 53.3 6.3 286.5 115.8 BB38RMQKZ × MM53 203.1 20.7 10.0 0.00.5 53.6 5.9 295.7 118.9 A3027RMQKZ × A2959LMSLZ 202.6 21.3 10.0 0.6 0.254.1 6.2 301.8 121.9 A3829RMQKZ × A6737Z 190.4 20.5 9.0 0.1 0.4 55.9 6.4283.5 125.0 Overall comparisons: Third Year field trials, 22 repsA7196GJLHZ × T6053LFWMZ 235.4 24.0 10.0 0.0 0.0 54.4 7.0 277.4 109.7CB15 × A1555RMQKZ 223.6 23.3 10.0 0.0 0.5 53.1 6.7 301.8 97.5 BB209 ×A1555RMQKZ 221.7 21.4 11.0 0.4 0.9 52.8 6.4 301.8 106.7 BB87 ×A1555RMQKZ 220.2 22.2 10.0 0.0 2.0 52.9 6.6 292.6 103.6 DKC62-97RIB219.7 21.9 10.0 0.1 0.7 55.1 6.5 283.5 103.6 BB38 × A1555RMQKZ 216.622.1 10.0 3.6 0.0 53.7 6.5 289.6 97.5 P1339AM1 214.9 22.8 10.0 2.0 2.155.1 6.3 317.0 118.9BB210

TABLE 4 Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA nComparative Data for 213UC00848 + VTU, a Hybrid Having BB210 as OneInbred Parent Overall comparisons: Year 1 field trials, 23 reps209UC00596 + VTU BB59 × A1555RMQKZ 186.1 19.7 9.5 53.8 0.0 1.5 3.2 9.46.4 9DKC62-97 DKC62-97 184.6 19.8 9.3 54.4 0.0 0.0 3.4 9.2 6.2213UC00848 + VTU BB210 × A1555RMQKZ 192.1 20.4 9.4 53.1 0.6 0.6 3.1 9.56.1 209UC00595 + VTU BB38 × A1555RMQKZ 186.6 20.5 9.1 53.6 0.8 0.9 3.49.5 6.4 9P1395AM1 P1395AM1 184.3 20.9 8.8 53.8 1.0 4.6 3.8 10.0 5.9Comparative Data for 213UC00848 + VTU, a Hybrid Having BB210 as OneInbred Parent Overall comparisons: Year 2 field trials, 57 reps213UC00848 + VTU 88210 × A1555RMQKZ 216.8 21.6 10.0 53.2 0.8 0.0 3.2 9.46.2 209UC00595 + VTU BB38 × A1555RMQKZ 216.1 21.6 10.0 53.7 0.4 0.0 3.49.6 6.2 9DKC62-97RIB 9DKC62-97RIB 215.7 22.0 9.8 55.0 0.0 0.0 3.2 9.16.3 9P1339AM1 9P1339AM1 212.7 22.6 9.4 55.2 3.2 0.0 3.9 10.1 5.8211UU01785 + VTU CB15 × A1555RMQKZ 215.6 22.8 9.5 53.4 0.9 0.1 3.1 9.56.1 Comparative Data for 213UC00848 + VTU, a Hybrid Having BB210 as OneInbred Parent Overall comparisons: Year 3 field trials, 64 reps9DKC63-33RIB 9DKC63-33R1B 220.9 19.8 11.2 55.7 1.7 1.6 3.4 9.0 5.4209UC00595 + VTU BB38 × A1555RMQKZ 215.7 20.8 10.4 53.8 0.5 0.7 3.1 9.26.0 213UC00848 + VTU BB210 × A1555RMQKZ 220.4 20.9 10.5 53.2 1.6 3.5 3.49.3 5.6 9P1221AMXT 9P1221AMXT 207.7 20.9 9.9 56.2 0.6 0.4 3.8 9.6 5.62110001785 + VTU CB15 × A1555RMQKZ 223.7 22.6 9.9 53.4 2.0 2.7 2.9 9.26.1BB211

TABLE 5 Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA nComparative Data for 213UC00851, a Hybrid Having BB211 as One InbredParent Overall comparisons: Year 1 field trials, 13 reps 213UC00851BB211 × ML9 174.1 16.6 10.5 56.6 0.0 5.0 2.9 8.2 6.2 208UN012309 BB38 ×ML9 173.6 17.9 9.7 55.4 0.1 2.3 2.7 7.9 6.3 9DKC57-50 DKC57-50 164.218.7 8.8 56.0 0.0 3.7 3.2 8.9 5.7 209UC00596 + VTU BB59 × A1555RMQKZ169.2 20.5 8.2 52.2 0.0 1.4 2.8 8.5 6.9 9P1018AM1 P1018AM1 165.2 20.58.0 54.2 0.0 14.4 3.4 9.3 6.4 Comparative Data for 213UC00851 and213UC01454, Hybrids Having BB211 as One Inbred Parent Overallcomparisons: Year 2 field trials, 24 reps 213UC01454 BB211 × MM53 205.819.1 10.8 52.6 0.8 0.2 4.2 10.0 5.6 213UC00851 BB211 × ML9 200.8 20.010.0 53.1 0.0 0.3 3.8 9.7 6.2 213UM02224 + GSS R2999RMQKB × T5842LMSLZ198.5 20.2 9.8 54.4 0.6 0.1 3.6 9.1 5.9 9DKC53-56RIB 9DKC53-56RIB 199.320.9 9.5 54.7 0.0 0.0 4.1 9.4 6.3 212UC00914 + VTU RBO1RMQKZ × MM53194.4 21.0 9.3 52.9 0.0 1.5 3.8 9.1 5.7 9P0533AM1 9P0533AM1 202.2 21.59.4 55.3 0.1 0.7 3.5 9.1 5.3 211UA02203 + VTU MEF2526RMQKZ × HCL4029191.1 21.7 8.8 55.0 0.0 0.5 3.5 9.1 5.6 Comparative Data for213UC00852 + VTU, a Hybrid Having BB211 as One Inbred Parent Overallcomparisons: Year 3 field trials, 60 reps 211US00896 + 017 BB38RMQKZ ×MM53 199.0 19.6 10.2 53.5 1.2 2.2 3.9 10.1 5.5 9DKC57-75RIB 9DKC57-75RIB199.6 20.1 9.9 54.3 0.8 0.5 3.6 9.4 5.9 213UA02359 + GSS A3027RMQKZ ×A2959LMSLZ 207.4 20.8 10.0 54.0 1.1 2.8 4.0 9.9 5.6 209UC00596 + VTUBB59 × A1555RMQKZ 205.1 20.9 9.8 52.7 0.5 2.9 3.6 9.5 6.0 213UC00852 +VTU BB211 × A1555RMQKZ 204.6 21.1 9.7 52.8 1.5 1.0 3.8 9.9 5.8 9P0832AMX9P0832AMX 200.4 21.8 9.2 55.1 0.7 11.3 3.8 9.7 5.8 212UA01994 + VTUT5056RMQKZ × T6540Z 206.8 22.0 9.4 54.7 1.3 5.0 3.9 9.5 5.7 214UA02497 +GSS T3997GJLHZ × T1540LFWMZ 202.0 22.8 8.9 53.8 0.6 8.8 4.0 9.8 6.2BC146

TABLE 6 Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA nComparative Data for 213UE00993, a Hybrid Having BC146 as One InbredParent Overall comparisons: Year 1 field trials, 4 reps 207UE00488 BB36× MN7 197.8 21.6 9.2 55.4 0.6 2.9 5.5 208UC00653 BB59 × LH287BT1CCR1192.7 22.0 8.8 55.0 0.3 23.8 5.2 213UE00993 BC146 × MN7 205.2 22.1 9.355.3 0.0 7.3 6.2 208UC00653 BB59 × LH287BT1CCR1 194.0 22.2 8.7 55.1 0.023.3 6.2 207UE00488 BB36 × MN7 201.9 22.5 9.0 54.6 0.0 1.5 5.5208UC00645 BB38 × LH287BT1CCR1 215.1 22.7 9.5 55.2 0.3 13.1 6.0208UC00645 BB38 × LH287BT1CCR1 188.1 23.3 8.1 55.5 0.0 22.2 6.0Comparative Data for 213UE00994, a Hybrid Having BC146 as One InbredParent Overall comparisons: Year 2 field trials, 15 reps 209UC00595 BB38× A1555RMQKZ 191.0 19.9 9.6 53.7 1.9 0.0 3.5 9.7 5.9 209UC00596 BB59 ×A1555RMQKZ 184.1 20.1 9.2 54.6 0.4 0.0 3.3 9.4 6.2 212UJ00007 BB87 ×A1555RMQKZ 193.8 21.2 9.1 53.5 1.2 2.2 3.4 9.5 6.4 211UU01785 CB15 ×A1555RMQKZ 190.6 21.6 8.8 54.3 0.4 0.0 3.3 9.5 6.6 212UC00811 BB36 ×A1555RMQKZ 194.1 21.7 8.9 52.9 1.1 1.5 3.4 9.4 6.5 213UE00994 BC146 ×A1555RMQKZ 197.3 22.0 9.0 54.2 1.4 0.3 3.6 9.7 6.6 Comparative Data for213UE00995, a Hybrid Having BC146 as One Inbred Parent Overallcomparisons: Year 2 field trials, 14 reps 211UC01094 CB18 × MN7RMQKZ179.7 19.1 9.4 54.3 0.6 0.0 3.4 9.1 5.4 209UC00596 BB59 × A1555RMQKZ182.9 20.7 8.8 52.8 0.5 0.3 3.8 9.5 6.4 213UE00995 BC146 × MN7RMQKZ195.0 20.8 9.4 52.3 2.5 0.0 3.8 9.5 6.0 209UC00595 BB38 × A1555RMQKZ191.7 20.9 9.2 54.5 1.3 0.6 3.5 9.6 5.9 Comparative Data for 213UE00995,a Hybrid Having BC146 as One Inbred Parent Overall comparisons: Year 3field trials, 22 reps 213UE00995 BC146 × MN7RMQKZ 212.2 20.2 10.5 52.01.3 0.4 3.4 9.9 6.1 9DKC62-97RIB 9DKC62-97RIB 217.1 21.8 9.9 54.9 0.20.0 3.6 9.6 6.7 212UJ00007 BB87 × A1555RMQKZ 224.7 22.2 10.1 52.6 0.00.0 3.5 9.9 6.8 209UC00595 BB38 × A1555RMQKZ 211.7 22.3 9.5 53.2 1.9 0.13.4 9.7 6.5 211UU01785 CB15 × A1555RMQKZ 220.4 23.3 9.5 53.3 0.0 0.1 3.410.0 6.6 21UU02222 A7196GJLHZ × T6053LFWMZ 223.1 23.7 9.4 54.6 0.0 0.03.3 8.9 6.5 Comparative Data for 213UE00995, a Hybrid Having BC146 asOne Inbred Parent Overall comparisons: Year 3 field trials, 22 reps213UE00995 BC146 × MN7RMQKZ 220.8 20.4 10.8 52.3 1.4 0.0 3.7 9.7 6.19DKC62-97RIB 9DKC62-97RIB 219.7 21.9 10.0 55.1 0.1 0.7 3.4 9.3 6.5209UC00595 BB38 × A1555RMQKZ 216.6 22.1 9.8 53.7 3.6 0.0 3.2 9.5 6.5212UJ00007 BB87 × A1555RMQKZ 220.2 22.2 9.9 52.9 0.0 2.0 3.4 9.6 6.69P1339AM1 9P1339AM1 214.9 22.8 9.4 55.1 2.0 2.1 3.9 10.4 6.3 211UU01785CB15 × A1555RMQKZ 223.6 23.3 9.6 53.1 0.0 0.5 3.2 9.9 6.7 213UU02222A7196GJLHZ × T6053LFWMZ 235.4 24.0 9.8 54.4 0.0 0.0 3.6 9.1 7.0Comparative Data for 213UE02417, a Hybrid Having BC146 as One InbredParent Overall comparisons: Year 3 field trials, 14 reps 211US00896BB38RMQKZ × MM53 217.7 20.2 10.8 53.5 0.0 1.1 3.6 9.7 5.7 211UC01094CB18 × MN7RMQKZ 207.4 20.4 10.2 52.1 0.0 0.0 3.7 9.8 5.9 213UE02417BC146 × MM53 215.6 20.6 10.4 52.8 0.5 2.0 3.9 9.5 5.9 211UV00415 BB46 ×MM27RMQKZ 200.6 21.6 9.3 53.1 0.0 2.6 4.0 9.1 6.1 213UA02359 A3027RMQKZ× A2959LMSLZ 206.7 22.2 9.3 54.1 0.0 0.7 3.8 9.2 6.2 209UC00596 BB59 ×A1555RMQKZ 216.2 23.1 9.4 52.4 0.0 0.4 3.3 9.0 6.4 Comparative Data for213UE00994 and 213UE00995, Hybrids Having BC146 as One Inbred ParentOverall comparisons: Year 3 field trials, 11 reps 213UE00995 BC146 ×MN7RMQKZ 211.7 18.8 11.2 52.3 0.0 0.0 3.4 9.7 5.8 211UV00423 BB36 ×MN7RMQKZ 195.9 18.9 10.4 51.5 0.0 0.0 3.1 10.0 6.3 211UV00415 BB46 ×MM27RMQKZ 201.2 19.9 10.1 53.7 0.3 0.0 3.4 9.3 5.8 212UJ00007 BB87 ×A1555RMQKZ 206.3 20.8 9.9 52.7 0.0 0.0 3.3 10.2 6.4 9DKC62-97RIB9DKC62-97R1B 211.3 20.9 10.1 54.6 0.0 0.0 3.4 9.7 6.8 209UC00595 BB38 ×A1555RMQKZ 212.6 21.5 9.9 53.3 0.0 0.0 3.1 9.4 6.7 213UE00994 BC146 ×A1555RMQKZ 212.4 21.5 9.9 53.7 0.0 0.0 3.6 10.0 6.3 212UC00811 BB36 ×A1555RMQKZ 211.4 22.1 9.6 52.7 0.0 0.0 3.1 9.4 6.2 9P1339AM1 9P1339AM1205.7 22.1 9.3 55.2 1.1 0.0 3.9 10.4 6.0 211UU01785 CB15 × A1555RMQKZ204.8 22.6 9.0 53.0 0.0 0.0 3.1 9.6 6.8 Comparative Data for 213UE02422,a Hybrid Having BC146 as One Inbred Parent Overall comparisons: Year 3field trials, 9 reps 211UV00415 BB46 × MM27RMQKZ 213.0 20.4 10.5 54.20.0 5.4 3.4 9.8 5.6 213UE02422 BC146 × MN26 216.8 20.8 10.4 55.6 0.0 0.53.4 9.8 6.3 9DKC62-97 9DKC62-97 216.6 21.5 10.1 55.7 0.0 0.9 3.4 9.7 6.2212UJ00007 BB87 × A1555RMQKZ 217.9 21.7 10.1 53.0 0.0 5.4 3.0 9.5 6.0209UC00595 BB38 × A1555RMQKZ 224.7 22.0 10.2 53.6 0.0 7.3 3.0 9.8 6.8212UC00811 BB36 × A1555RMQKZ 216.1 22.4 9.7 53.3 0.2 11.0 2.8 9.8 6.2213UU02222 A7196GJLHZ × T6053LFWMZ 226.8 23.0 9.9 55.7 0.0 1.1 3.4 9.46.3 211UU01785 CB15 × A1555RMQKZ 216.1 23.5 9.2 53.2 0.0 5.9 3.0 9.7 6.4Comparative Data for 213UE00994, a Hybrid Having BC146 as One InbredParent Overall comparisons: Year 4 field trials, 17 reps 214UA02497T3997GJLHZ × T1540LFWMZ 205.4 19.7 10.4 56.2 0.5 0.0 3.9 9.8 6.1209UC00595 BB38 × A1555RMQKZ 229.7 19.9 11.6 55.0 0.0 0.0 3.4 9.3 6.3212UJ00007 BB87 × A1555RMQKZ 230.1 19.9 11.6 54.1 3.6 0.0 3.5 9.4 6.2213UE00994 BC146 × A1555RMQKZ 223.3 20.1 11.1 54.5 0.0 0.0 3.4 9.5 6.39P1498HR 9P1498HR 214.2 20.5 10.4 56.6 0.0 0.0 3.8 9.9 6.0 213UC01433BB36GJLHZ × A1555LFWMZ 215.1 20.7 10.4 54.1 2.7 0.0 3.4 9.7 6.3213UA02371 F7316RMQKZ × F1266Z 225.1 20.7 10.9 55.9 0.0 0.0 3.8 9.0 6.2213UU02222 A7196GJLHZ × T6053LFWMZ 222.5 21.5 10.3 55.7 1.3 1.9 3.5 9.05.7 9P1690HR 9P1690HR 224.2 22.2 10.1 55.9 5.8 0.0 3.8 10.0 6.1Comparative Data for 213UE00994, a Hybrid Having BC146 as One InbredParent Overall comparisons: Year 4 field trials, 64 reps 9DKC63-33RIB9DKC63-33R18 220.9 19.8 11.2 55.7 1.7 1.6 3.4 9.0 5.4 209UC00595 BB38 ×A1555RMQKZ 215.7 20.8 10.4 53.8 0.5 0.7 3.1 9.2 6.0 213UE00994 BC146 ×A1555RMQKZ 220.9 20.8 10.6 53.4 0.9 2.1 3.3 9.3 5.8 9P1221AMXT9P1221AMXT 207.7 20.9 9.9 56.2 0.6 0.4 3.8 9.6 5.6 212UJ00007 BB87 ×A1555RMQKZ 222.4 21.1 10.5 53.1 2.2 2.0 3.3 9.3 6.1 213UA02371F7316RMQKZ × F1266Z 229.3 21.3 10.8 55.1 0.3 1.1 3.6 9.0 6.1 213UC01433BB36GILHZ × A1555LFWMZ 217.1 22.1 9.8 52.8 1.3 1.3 3.3 9.2 6.1213UA02677 F2608RMQKZ × T9551LMSLZ 219.2 22.2 9.9 55.7 0.4 1.1 3.4 9.56.1 211UU01785 CB15 × A1555RMQKZ 223.7 22.6 9.9 53.4 2.0 2.7 2.9 9.2 6.1213UU02222 A7196GJLHZ × T6053LFWMZ 230.6 22.9 10.1 55.1 0.4 0.5 3.3 8.66.4BC147

TABLE 7 Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA nComparative Data for 213UE03466, a Hybrid Having BC147 as One InbredParent Overall comparisons: Year 1 field trials, 4 reps 208UC00653 BB59× LH287BT1CCR1 181.9 22.7 8.0 57.7 0.0 9.1 6.1 208UC00653 BB59 ×LH287BT1CCR1 193.9 23.3 8.3 57.2 0.0 4.6 6.6 213UE03466 BC147 × MN7210.6 24.0 8.8 56.6 0.1 0.6 6.2 207UE00488 BB36 × MN7 198.7 24.3 8.256.7 0.0 3.7 5.8 208UC00645 BB38 × LH287BT1CCR1 203.9 24.6 8.3 56.9 0.03.7 7.3 207UE00488 BB36 × MN7 186.9 24.6 7.6 57.1 0.1 14.2 6.5208UC00645 BB38 × LH287BT1CCR1 202.3 25.0 8.1 57.8 0.0 4.7 7.3Comparative Data for 213UE01007, a Hybrid Having BC147 as One InbredParent Overall comparisons: Year 2 field trials, 15 reps 209UC00595 BB38× A1555RMQKZ 191.0 19.9 9.6 53.7 1.9 0.0 3.5 9.7 5.9 209UC00596 BB59 ×A1555RMQKZ 184.1 20.1 9.2 54.6 0.4 0.0 3.3 9.4 6.2 212UJ00007 BB87 ×A1555RMQKZ 193.8 21.2 9.1 53.5 1.2 2.2 3.4 9.5 6.4 211UU01785 CB15 ×A1555RMQKZ 190.6 21.6 8.8 54.3 0.4 0.0 3.3 9.5 6.6 212UC00811 BB36 ×A1555RMQKZ 194.1 21.7 8.9 52.9 1.1 1.5 3.4 9.4 6.5 213UE01007 BC147 ×A1555RMQKZ 202.7 22.1 9.2 54.2 5.0 0.0 3.6 10.1 6.7 Comparative Data for213UE01008, a Hybrid Having BC147 as One Inbred Parent Overallcomparisons: Year 2 field trials, 15 reps 211UC01094 CB18 × MN7RMQKZ183.5 18.6 9.9 54.4 1.1 0.0 3.6 9.0 5.1 209UC00595 BB38 × A1555RMQKZ193.4 20.7 9.4 54.4 1.1 1.2 3.5 9.4 6.4 213UE01008 BC147 × MN7RMQKZ198.8 20.7 9.6 53.5 0.5 0.6 3.9 9.9 6.3 209UC00596 BB59 × A1555RMQKZ186.9 20.9 8.9 54.1 0.8 0.1 3.7 9.6 6.1 Comparative Data for 213UE01008,a Hybrid Having BC147 as One Inbred Parent Overall comparisons: Year 3field trials, 24 reps 211UC01094 CB18 × MN7RMQKZ 210.9 20.0 10.5 51.90.0 0.0 3.7 9.3 6.0 211US00896 BB38RMQKZ × MM53 203.1 20.7 9.8 53.6 0.00.5 3.9 9.7 5.9 211UV00415 BB46 × MM27RMQKZ 204.8 21.2 9.7 53.3 0.0 3.13.8 9.4 6.3 213UA02359 A3027RMQKZ × A2959LMSLZ 202.6 21.3 9.5 54.1 0.60.2 4.0 9.9 6.2 9P0832AMX 9P0832AMX 207.1 21.4 9.7 55.6 0.1 5.8 4.3 9.55.8 9DKC57-75RIB 9DKC57-75RIB 205.7 21.8 9.4 54.3 0.0 2.2 3.5 8.8 6.6209UC00596 BB59 × A1555RMQKZ 212.2 21.8 9.7 51.9 0.0 1.2 3.7 9.2 6.8213UE01008 BC147 × MN7RMQKZ 215.1 23.4 9.2 50.9 0.0 0.9 4.1 10.1 6.7Comparative Data for 213UE01007 and 213UE01008, Hybrids Having BC147 asOne Inbred Parent Overall comparisons: Year 3 field trials, 23 reps212UJ00007 BB87 × A1555RMQKZ 217.8 21.5 10.1 52.7 2.7 1.1 3.8 9.9 6.19DKC62-97RIB 9DKC62-97RIB 217.8 21.6 10.1 55.2 1.2 0.0 3.5 9.4 6.4209UC00595 BB38 × A1555RMQKZ 220.5 21.6 10.2 53.7 0.5 0.2 3.4 9.8 6.2213UE01008 BC147 × MN7RMQKZ 219.2 21.9 10.0 51.8 1.5 0.0 3.7 9.9 6.4213UE01007 BC147 × A1555RMQKZ 219.8 22.7 9.7 53.0 2.6 0.7 3.5 10.0 6.79P1339AM1 9P1339AM1 214.3 22.9 9.4 55.3 1.6 0.1 3.8 10.1 6.3 211UU01785CB15 × A1555RMQKZ 216.0 23.5 9.2 52.9 1.6 0.3 3.4 9.9 6.7 213UU02222A7196GJLHZ × T6053LFWMZ 231.4 23.6 9.8 54.5 0.3 0.0 4.0 9.1 6.8Comparative Data for 213UE01008, a Hybrid Having BC147 as One InbredParent Overall comparisons: Year 3 field trials, 22 reps 213UE01008BC147 × MN7RMQKZ 217.9 21.3 10.2 51.6 4.0 0.2 3.5 10.0 6.6 9DKC62-97RIB9DKC62-97RIB 217.1 21.8 9.9 54.9 0.2 0.0 3.6 9.6 6.7 212UJ00007 BB87 ×A1555RMQKZ 224.7 22.2 10.1 52.6 0.0 0.0 3.5 9.9 6.8 209UC00595 BB38 ×A1555RMQKZ 211.7 22.3 9.5 53.2 1.9 0.1 3.4 9.7 6.5 211UU01785 CB15 ×A1555RMQKZ 220.4 23.3 9.5 53.3 0.0 0.1 3.4 10.0 6.6 213UU02222A7196GJLHZ × T6053LFWMZ 223.1 23.7 9.4 54.6 0.0 0.0 3.3 8.9 6.5Comparative Data for 213UE01007, a Hybrid Having BC147 as One InbredParent Overall comparisons: Year 3 field trials, 22 reps 9DKC62-97RIB9DKC62-97RIB 219.7 21.9 10.0 55.1 0.1 0.7 3.4 9.3 6.5 209UC00595 BB38 ×A1555RMQKZ 216.6 22.1 9.8 53.7 3.6 0.0 3.2 9.5 6.5 212UJ00007 BB87 ×A1555RMQKZ 220.2 22.2 9.9 52.9 0.0 2.0 3.4 9.6 6.6 9P1339AM1 9P1339AM1214.9 22.8 9.4 55.1 2.0 2.1 3.9 10.4 6.3 213UE01007 BC147 × A1555RMQKZ226.9 23.2 9.8 52.7 0.0 6.7 3.5 10.2 6.9 211UU01785 CB15 × A1555RMQKZ223.6 23.3 9.6 53.1 0.0 0.5 3.2 9.9 6.7 213UU02222 A7196GJLHZ ×T6053LFWMZ 235.4 24.0 9.8 54.4 0.0 0.0 3.6 9.1 7.0 Comparative Data for213UE02418, a Hybrid Having BC147 as One Inbred Parent Overallcomparisons: Year 3 field trials, 14 reps 211US00896 BB38RMQKZ × MM53217.7 20.2 10.8 53.5 400.0 1.1 3.6 9.7 5.7 211UC01094 CB18 × MN7RMQKZ207.4 20.4 10.2 52.1 0.0 0.0 3.7 9.8 5.9 211UV00415 BB46 × MM27RMQKZ200.6 21.6 9.3 53.1 0.0 2.6 4.0 9.1 6.1 213UA02359 A3027RMQKZ ×A2959LMSLZ 206.7 22.2 9.3 54.1 0.0 0.7 3.8 9.2 6.2 213UE02418 BC147 ×MM53 215.7 22.5 9.6 52.4 0.3 3.8 4.2 9.9 6.1 209UC00596 BB59 ×A1555RMQKZ 216.2 23.1 9.4 52.4 0.0 0.4 3.3 9.0 6.4 Comparative Data for213UE01007 and 213UE01008, Hybrids Having BC147 as One Inbred ParentOverall comparisons: Year 3 field trials, 11 reps 211UV00423 BB36 ×MN7RMQKZ 195.9 18.9 10.4 51.5 0.0 0.0 3.1 10.0 6.3 211UV00415 BB46 ×MM27RMQKZ 201.2 19.9 10.1 53.7 0.3 0.0 3.4 9.3 5.8 213UE01008 BC147 ×MN7RMQKZ 205.4 20.4 10.1 51.7 0.0 0.0 3.4 9.8 6.4 212UJ00007 BB87 ×A1555RMQKZ 206.3 20.8 9.9 52.7 0.0 0.0 3.3 10.2 6.4 9DKC62-97RIB9DKC62-97RIB 211.3 20.9 10.1 54.6 0.0 0.0 3.4 9.7 6.8 209UC00595 BB38 ×A1555RMQKZ 212.6 21.5 9.9 53.3 0.0 0.0 3.1 9.4 6.7 212UC00811 BB36 ×A1555RMQKZ 211.4 22.1 9.6 52.7 0.0 0.0 3.1 9.4 6.2 9P1339AM1 9P1339AM1205.7 22.1 9.3 55.2 1.1 0.0 3.9 10.4 6.0 213UE01007 BC147 × A1555RMQKZ213.9 22.5 9.5 52.8 0.0 0.0 3.0 10.1 7.0 211UU01785 CB15 × A1555RMQKZ204.8 22.6 9.0 53.0 0.0 0.0 3.1 9.6 6.8 Comparative Data for 213UE01007,a Hybrid Having BC147 as One Inbred Parent Overall comparisons: Year 4field trials, 17 reps 214UA02497 T3997GJLHZ × T1540LFWMZ 205.4 19.7 10.456.2 0.5 0.0 3.9 9.8 6.1 209UC00595 BB38 × A1555RMQKZ 229.7 19.9 11.655.0 0.0 0.0 3.4 9.3 6.3 212UJ00007 BB87 × A1555RMQKZ 230.1 19.9 11.654.1 3.6 0.0 3.5 9.4 6.2 213UE01007 BC147 × A1555RMQKZ 230.3 20.5 11.254.7 0.0 0.0 3.5 9.6 6.5 9P1498HR 9P1498HR 214.2 20.5 10.4 56.6 0.0 0.03.8 9.9 6.0 213UC01433 BB36GJLHZ × A1555LFWMZ 215.1 20.7 10.4 54.1 2.70.0 3.4 9.7 6.3 213UA02371 F7316RMQKZ × F1266Z 225.1 20.7 10.9 55.9 0.00.0 3.8 9.0 6.2 213UU02222 A7196GJLHZ × T6053LFWMZ 222.5 21.5 10.3 55.71.3 1.9 3.5 9.0 5.7 9P1690HR 9P1690HR 224.2 22.2 10.1 55.9 5.8 0.0 3.810.0 6.1 Comparative Data for 214UN02889, a Hybrid Having BC147 as OneInbred Parent Overall comparisons: Year 4 field trials, 22 reps211US00896 BB38RMQKZ × MM53 200.4 19.4 10.3 54.0 0.6 7.7 3.6 10.5 5.49DKC57-75RIB 9DKC57-75RIB 198.5 20.3 9.8 54.6 0.3 10.1 3.2 9.1 6.3214UN02889 BC147 × MM64 212.0 20.4 10.4 52.4 0.4 5.6 3.7 10.2 6.4213UA02359 A3027RMQKZ × A2959LMSLZ 206.0 20.4 10.1 54.4 0.4 1.6 3.8 10.15.9 209UC00596 BB59 × A1555RMQKZ 202.2 20.8 9.7 52.5 0.0 0.3 3.6 10.25.6 212UA01994 T5056RMQKZ × T6540Z 209.5 21.3 9.9 55.2 0.4 4.7 3.5 9.45.8 214UA02497 T3997GJLHZ × T1540LFWMZ 213.0 22.8 9.3 53.8 0.1 4.8 4.410.2 6.9 212UE01615 R2613RMQKZ × MN26 202.6 23.0 8.8 55.7 0.8 5.5 3.410.0 5.9 Comparative Data for 214UE03317, a Hybrid Having BC147 as OneInbred Parent Overall comparisons: Year 4 field trials, 25 reps213UU03346 F7316RPGJZ × F1266Z 220.9 19.8 11.1 55.5 0.8 2.1 3.3 8.6 5.2214UE03317 BC147 × A1555RPGJZ 226.2 19.9 11.4 54.1 1.4 1.3 3.3 9.0 5.8213UU03348 F2608RPGJZ × T9551Z 223.0 20.4 10.9 56.2 2.5 0.5 3.6 9.3 5.4210UJ02224 BB36RR2 × A1555ZNYKZ 217.4 20.4 10.6 54.6 0.4 0.1 3.0 9.0 5.4210UU00473 CB15 × LH287 211.4 20.8 10.2 54.5 0.6 1.1 2.9 9.0 5.9214UA03018 A7196RPGJZ × T6053Z 224.1 20.9 10.7 56.8 1.5 0.0 3.5 8.7 5.69P1360AM 9P1360AM 219.8 21.0 10.5 56.7 0.8 6.0 3.2 9.0 5.4 212UU00615CB15RHTTZ × A1555ZNYKZ 220.1 21.4 10.3 54.3 0.4 1.0 2.9 8.8 5.8214UA03131 A7196RPGJZ × A9826Z 215.6 22.3 9.7 55.2 0.4 0.0 3.3 8.8 5.79DKC66-97RIB 9DKC66-97RIB 216.8 22.4 9.7 55.3 0.1 0.0 3.3 8.9 6.0213UA02691 A7196RPGJZ × F1479Z 221.0 22.5 9.8 55.6 10.5 0.0 3.8 9.3 6.79P1690HR 9P1690HR 228.7 22.7 10.1 56.1 0.9 5.5 3.6 9.6 6.5 ComparativeData for 213UE01007, a Hybrid Having BC147 as One Inbred Parent Overallcomparisons: Year 4 field trials, 57 reps 213UU03346 F7316RPGJZ × F1266Z219.8 20.4 10.8 55.7 0.8 0.0 3.3 8.6 5.6 213UU03348 F2608RPGJZ × T9551Z220.3 21.0 10.5 55.7 1.0 2.2 3.5 9.0 5.5 9DKC64-69 9DKC64-69 212.3 21.210.0 55.9 1.7 1.7 3.6 8.8 5.2 213UE01007 BC147 × A1555RMQKZ 223.7 21.410.4 53.6 0.6 1.7 3.3 9.2 6.0 214UA03018 A7196RPGJZ × T6053Z 216.3 21.510.1 56.6 2.0 1.4 3.4 8.8 5.4 9P1360AM 9P1360AM 210.7 21.5 9.8 56.3 1.72.9 3.4 9.1 5.5 210UJ02224 BB36RR2 × A1555ZNYKZ 208.0 21.5 9.7 54.3 0.01.0 2.9 8.8 5.5 212UU00615 CB15RHTTZ × A1555ZNYKZ 218.0 21.7 10.0 54.31.8 1.0 3.3 9.1 5.4 9DKC66-97RIB 9DKC66-97RIB 216.5 22.7 9.5 54.8 1.00.0 3.3 9.1 5.8 9P1690HR 9P1690HR 223.3 22.9 9.7 55.2 3.1 1.8 3.7 9.76.2 214UA03131 A7196RPGJZ × A9826Z 216.2 23.2 9.3 54.7 1.0 0.1 3.4 9.15.7 213UA02691 A7196RPGJZ × F1479Z 207.2 23.2 8.9 55.0 0.9 1.3 3.6 9.46.4CB21

TABLE 8 Comparative Data for 508UV07587, a Hybrid Having CB21 as OneInbred Parent Overall comparisons: Year 1 field trials, 4 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % HA n 508UV07587 CB21/MN7 218.318.7 11.7 55.4 0.3 1.3 5.5 208UN01154 + NZR BB14BT1RR2/MN7 216.2 18.811.5 55.4 0.3 0.0 5.0 207US00957 BB38/MN7 210.0 19.4 10.8 55.9 −0.1 0.75.0 Comparative Data for 211UV00440, a Hybrid Having CB21 as One InbredParent Overall comparisons: Year 2 field trials, 21 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHt PIHt HA n 211UV00440 CB21/ML9190.3 17.4 10.9 54.8 1.4 0.4 3.8 9.7 5.8 206UV00328 BB14/MM27 182.3 19.29.5 54.6 2.6 0.0 3.4 9.2 5.6 208UN01239 BB38/ML9 184.0 19.7 9.3 54.8 0.60.0 3.4 9.9 6.4 211US00002 + 603 BB59/LH287RR2-1 187.6 19.7 9.5 54.8 1.00.1 3.4 9.7 6.6 9DKC61-69 DKC61-69 189.0 20.0 9.4 55.0 2.2 1.9 4.0 10.15.9 Comparative Data for 211UV00440, a Hybrid Having CB21 as One InbredParent Overall comparisons: Year 3 field trials, 22 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHt PIHt HA n 211UV00440 CB21/ML9187.3 18.7 10.0 56.6 0.7 4.5 3.4 8.8 5.4 208UN01239 BB38/ML9 178.3 18.79.5 57.7 1.0 1.4 3.0 8.9 5.6 9DKC57-50 DKC57-50 179.3 19.7 9.1 57.1 0.51.7 3.5 9.1 5.6 211US00002 + 603 BB59/LH287RR2-1 181.4 20.7 8.8 56.5 1.53.8 3.5 9.1 5.9 208UC00653 + YGC BB59/LH287BT1CCR1 185.6 20.8 8.9 55.92.7 3.9 3.5 9.3 6.0 Comparative Data for 211UV01591 + VTU, a HybridHaving CB21 as One Inbred Parent Overall comparisons: Year 4 fieldtrials, 8 reps Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % HA n211UC01091 + 017 BC110/MN7RMQKZ 146.0 19.2 7.6 53.9 0.0 0.0 4.7211UC01094 + 017 CB18/MN7RMQKZ 171.9 21.0 8.2 52.6 0.2 0.0 4.7211UV01591 + VTU CB21/A1555RMQKZ 175.7 22.2 7.9 52.1 0.1 0.0 5.7209UC00595 + VTU BB38/A1555RMQKZ 170.7 23.3 7.3 51.3 0.0 0.0 5.7Comparative Data for 211UV01591 + VTU, a Hybrid Having CB21 as OneInbred Parent Overall comparisons: Year 5 field trials, 64 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 211UC01094 + 017CB18 × MN7RMQKZ 200.7 19.7 10.2 52.3 0.1 0.1 3.5 9.2 6.0 211US00896 +017 BB38RMQKZ × MM53 195.1 20.1 9.7 53.9 0.2 0.4 3.7 9.5 5.79DKC57-75RIB 9DKC57-75RIB 203.2 20.9 9.7 54.7 0.3 1.2 3.3 8.7 6.59P0832AMX 9P0832AMX 199.5 21.3 9.4 55.8 0.5 3.7 3.9 9.5 5.8 209UC00596 +VTU BB59 × A1555RMQKZ 203.4 21.4 9.5 52.7 0.0 1.7 3.4 8.9 6.5211UV01591 + VTU CB21/A1555RMQKZ 210.0 21.6 9.7 53.0 0.8 2.0 3.6 8.6 6.5211UV00415 + 017 BB46 × MM27RMQKZ 201.1 21.6 9.3 53.5 0.6 1.1 3.8 9.36.1 Comparative Data for 211UV01591 + VTU, a Hybrid Having CB21 as OneInbred Parent Overall comparisons: Year 5 field trials, 61 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 211US00896 + 017BB38RMQKZ × MM53 199.3 20.1 9.9 53.5 0.5 0.4 3.6 9.5 5.9 211UC01094 +017 CB18 × MN7RMQKZ 207.2 20.5 10.1 51.4 0.3 0.0 3.5 9.1 6.09DKC57-75RIB 9DKC57-75RIB 199.2 21.2 9.4 54.5 0.1 0.6 3.3 8.6 6.5211UV00415 + 017 BB46 × MM27RMQKZ 208.5 21.4 9.7 53.0 0.4 1.2 3.7 9.36.1 209UC00596 + VTU BB59 × A1555RMQKZ 208.1 21.7 9.6 52.3 0.1 0.8 3.49.3 6.5 211UV01591 + VTU CB21/A1555RMQKZ 213.2 21.9 9.7 52.7 0.0 0.8 3.79.2 6.7 Comparative Data for 211UV00440, a Hybrid Having CB21 as OneInbred Parent Overall comparisons: Year 6 field trials, 58 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 211UV00440 CB21 ×ML9 200.2 17.4 11.8 52.8 0.8 4.8 3.7 9.1 5.5 212UC00914 + VTU RBO1RMQKZ× MM53 194.6 18.3 10.9 53.2 0.8 1.2 3.7 9.5 5.1 9DKC53-56RIB DKC53-56RIB193.5 19.5 10.2 54.7 0.2 2.6 3.3 9.0 5.2 9P0533AM1 P0533AM1 199.9 21.09.7 55.6 0.8 2.6 3.1 8.6 4.7 Comparative Data for 215UV00062, a HybridHaving CB21 as One Inbred Parent Overall comparisons: Year 6 fieldtrials, 60 reps Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHTHA n 215UV00062 CB21 × MM69 202.7 17.7 12.1 52.1 3 0.9 3.9 9.9 5.5211US00896 + 017 BB38RMQKZ × MM53 199 19.6 10.8 53.5 1.2 2.2 3.9 10.15.5 9DKC57-75RIB 9DKC57-75RIB 199.6 20.1 10.3 54.3 0.8 0.5 3.6 9.4 5.9209UC00596 + VTU BB59 × A1555RMQKZ 205.1 20.9 10.3 52.7 0.5 2.9 3.6 9.56 9P0832AMX 9P0832AMX 200.4 21.8 9.5 55.1 0.7 11.3 3.8 9.7 5.8CB34

TABLE 2 Comparative Data for 213UC00864 + VTU, a Hybrid Having CB34 asOne Inbred Parent Overall comparisons: Year 1 field trials, 23 repsGenotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n210US02194 + VTU F4780RMQKZ × T5972Z 178.2 17.3 10.3 57.8 0.6 0.0 3.28.5 5.4 211US00896 + 017 BB38RMQKZ × MM53 192.2 18.1 10.6 55.4 0.0 0.03.3 8.8 5.9 211UC01094 + 017 CB18 × MN7RMQKZ 191.4 18.7 10.2 54.5 0.40.0 3.1 8.2 5.3 211UV00415 + 017 BB46 × MM27RMQKZ 197.6 19.6 10.1 53.50.3 0.0 3.3 8.7 6.0 209UC00596 + VTU BB59 × A1555RMQKZ 183.3 20.7 8.952.3 0.4 0.2 2.7 8.3 6.2 213UC00864 + VTU CB34 × A1555RMQKZ 205.6 21.59.6 52.1 0.4 0.7 3.3 8.8 6.2

TABLE 3 Comparative Data for 213UC00864 + VTU, a Hybrid Having CB34 asOne Inbred Parent Overall comparisons: Year 2 field trials, 57 repsGenotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n213UC00864 + VTU CB34 × A1555RMQKZ 218.5 21.2 10.3 53.0 1.6 0.2 3.7 10.06.3 209UC00595 + VTU BB38 × A1555RMQKZ 216.1 21.6 10.0 53.7 0.4 0.0 3.49.6 6.2 212UJ00007 + VTU BB87 × A1555RMQKZ 217.4 21.8 10.0 52.8 1.5 0.23.1 9.5 6.4 9DKC62-97RIB 9DKC62-97RIB 215.7 22.0 9.8 55.0 0.0 0.0 3.29.1 6.3 9P1339AM1 9P1339AM1 212.7 22.6 9.4 55.2 3.2 0.0 3.9 10.1 5.8211UU01785 + VTU CB15 × A1555RMQKZ 215.6 22.8 9.5 53.4 0.9 0.1 3.1 9.56.1

TABLE 4 Comparative Data for 213UC00864 + VTU, a Hybrid Having CB34 asOne Inbred Parent Overall comparisons: Year 3 field trials, 64 repsGenotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n9DKC63-33RIB 9DKC63-33RIB 220.9 19.8 11.2 55.7 1.7 1.6 3.4 9.0 5.4209UC00595 + VTU BB38 × A1555RMQKZ 215.7 20.8 10.4 53.8 0.5 0.7 3.1 9.26.0 9P1221AMXT 9P1221AMXT 207.7 20.9 9.9 56.2 0.6 0.4 3.8 9.6 5.6213UC00864 + VTU CB34 × A1555RMQKZ 224.0 21.0 10.7 53.2 1.9 3.2 3.7 9.86.2 212UJ00007 + VTU BB87 × A1555RMQKZ 222.4 21.1 10.5 53.1 2.2 2.0 3.39.3 6.1 213UA02677 + GSS F2608RMQKZ × T9551LMSLZ 219.2 22.2 9.9 55.7 0.41.1 3.4 9.5 6.1 211UU01785 + VTU CB15 × A1555RMQKZ 223.7 22.6 9.9 53.42.0 2.7 2.9 9.2 6.1CB39

TABLE 10 Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA nComparative Data for 213UU00787 + VTU, a Hybrid Having CB39 as OneInbred Parent Overall comparisons: 60 reps 211US00896 + 017 BB38RMQKZ ×MM53 199.0 19.6 10.2 53.5 1.2 2.2 3.9 10.1 5.5 9DKC57-75RIB 9DKC57-75RIB199.6 20.1 9.9 54.3 0.8 0.5 3.6 9.4 5.9 213UA02359 + GSS 2201 × 8001207.4 20.8 10.0 54.0 1.1 2.8 4.0 9.9 5.6 209UC00596 + VTU BB59 × 7610205.1 20.9 9.8 52.7 0.5 2.9 3.6 9.5 6.0 9P0832AMX 9P0832AMX 200.4 21.89.2 55.1 0.7 11.3 3.8 9.7 5.8 212UA01994 + VTU 2036 × 8028 206.8 22.09.4 54.7 1.3 5.0 3.9 9.5 5.7 213UU00787 + VTU CB39 × 7610 201.3 22.1 9.152.6 0.7 2.8 3.5 9.9 6.0 214UA02497 + GSS 2349 × 8097 202.0 22.8 8.953.8 0.6 8.8 4.0 9.8 6.2 Comparative Data for 213UU00787 + VTU, a HybridHaving CB39 as One Inbred Parent Overall comparisons: 48 reps210UJ02225 + N34 BB59RR2 × 7610 201.5 19.9 10.1 53.6 0.0 1.9 6.3211US00896 + 017 BB38RMQKZ × MM53 207.5 20.1 10.3 54.3 0.7 2.2 5.9213UA02370 + GSS 2332 × 8077 192.0 20.1 9.6 55.9 2.0 2.0 5.9213UC00852 + VTU BB211 × 7610 203.9 20.8 9.8 53.7 0.1 2.1 6.1211UV01591 + VTU CB21 × 7610 205.9 21.2 9.7 53.8 0.2 2.2 6.4209UC00596 + VTU BB59 × 7610 203.7 21.2 9.6 53.2 0.0 1.6 6.3213UA02520 + GSS 2160 × 8013 188.8 21.4 8.8 55.7 0.1 2.0 6.2212UC00810 + VTU BB202 × 7610 205.4 21.9 9.4 54.3 0.1 2.4 6.4213UU00787 + VTU CB39 × 7610 206.9 22.1 9.4 53.2 0.1 2.3 6.5214UA02497 + GSS 2349 × 8097 199.0 22.2 9.0 54.6 0.0 3.4 6.6II15

TABLE 11 Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA nComparative data for 509UM06823, a Hybrid having II15 as One InbredParent Overall comparisons: Year 1 field trials, 7 reps 203UK00780 + 603HC50RR2-1/SGI901 176.0 21.4 8.2 54.9 0.9 0.4 5.4 209UM00819 + 603HCL116RR2/HCL419 202.4 23.1 8.8 53.9 1.8 0.6 6.4 509UM06823 AB7/II15201.1 23.6 8.5 53.4 0.0 0.7 5.4 206UM01239 + 603 HCL107RR2/HCL506 195.625.5 7.7 53.1 0.5 0.6 6.1 208UC01430 + 603 HCL105RR2/HCL531 204.6 27.27.5 52.4 0.0 0.0 6.7 Comparative data for 509UM06823, a Hybrid havingII15 as One Inbred Parent Overall comparisons: Year 2 field trials, 15reps 509UM06823 AB7/II15 170.2 14.5 11.8 58.2 4.0 2.9 3.1 8.7 5.3207UM01824 + 603 HC50RR2-1/HCL422 157.5 14.8 10.6 59.0 4.5 0.0 3.4 9.05.4 208UA01447 + YGC HCL116CCR1/HCL425BT1 164.7 15.5 10.6 59.1 1.3 0.02.9 7.6 4.9 9DKC43-27 DKC43-27 180.8 16.3 11.1 57.6 0.6 0.0 3.2 8.1 6.1209UM00819 + 603 HCL116RR2/HCL419 173.0 16.6 10.4 57.4 5.4 0.1 3.4 8.95.7 208UC01435 + YGC HCL116CCR1/HCL419BT1-2 185.9 17.2 10.8 57.2 2.5 0.03.5 9.0 6.4 206UM01239 + 603 HCL107RR2/HCL506 180.6 17.3 10.4 56.7 4.60.4 3.7 8.7 6.7 210UM01241 + 603 HCL112RR2/HCL437 185.8 18.4 10.1 56.81.3 0.3 3.3 8.7 6.5 Comparative data for 511UM07776, a Hybrid havingII15 as One Inbred Parent Overall comparisons: Year 3 field trials, 8reps 202UQ01053 + 603 HC37RR2-1/SGI918 127.3 18.8 6.8 55.9 3.8 0.4 3.07.9 5.1 210UK00575 + NZR LM3/IV2BT1RR2 98.6 19.5 5.1 56.1 1.9 1.8 2.87.2 4.9 9DKC30-20 DKC30-20 122.8 19.5 6.3 57.0 1.4 0.6 3.4 8.6 5.59P39D97 P39D97 137.4 19.6 7.0 58.2 3.2 19.6 3.6 7.8 4.6 208UA01447 + YGCHCL116CCR1/HCL425BT1 125.8 19.8 6.4 56.3 2.2 0.8 3.2 8.2 5.5207UM01824 + 603 HC50RR2-1/HCL422 136.0 19.9 6.8 55.9 2.9 0.9 3.2 8.65.8 9DKC35-43 DKC35-43 134.8 20.2 6.7 55.8 1.1 0.2 3.6 8.5 5.8210UJ01450 + 603 HC37RR2-1/SGI901 120.7 20.3 5.9 55.8 2.1 0.4 3.5 8.75.6 209UM00819 + 603 HCL116RR2/HCL419 136.2 20.7 6.6 56.1 6.1 2.8 3.68.7 5.9 511UM07776 AB19/II15 135.7 22.6 6.0 52.8 4.2 19.1 3.4 9.0 4.5Comparative data for 509UM06823 and 511UM07776, Hybrids Having II15 asOne Inbred Parent Overall comparisons: Year 3 field trials, 9 reps202UQ01053 + 603 HC37RR2-1/SGI918 122.2 18.2 6.7 56.9 5.6 0.1 3.1 7.84.9 9DKC35-43 DKC35-43 117.6 18.3 6.4 57.2 3.5 0.1 3.1 7.8 5.2509UM06823 AB7/II15 138.8 18.3 7.6 54.8 7.2 2.2 2.9 8.3 5.1 208UA01447 +YGC HCL116CCR1/HCL425BT1 126.6 18.4 6.9 57.1 1.8 0.1 2.8 7.4 5.1207UM01824 + 603 HC50RR2-1/HCL422 140.8 18.6 7.6 56.4 4.2 0.0 3.2 8.15.6 209UM00819 + 603 HCL116RR2/HCL419 126.2 18.9 6.7 57.1 9.3 0.1 3.28.2 5.3 9P8906HR P8906HR 132.2 19.2 6.9 56.9 2.0 0.1 3.4 8.2 5.79DKC38-89 DKC38-89 147.5 19.8 7.4 56.5 0.3 0.0 3.0 8.3 5.9 208UC01435 +YGC HCL116CCR1/HCL419BT1-2 137.6 19.9 6.9 56.7 6.2 0.1 3.4 8.4 5.8511UM07776 AB19/II15 142.4 20.1 7.1 54.9 1.4 12.3 3.3 8.6 5.0206UM01239 + 603 HCL107RR2/HCL506 143.9 21.2 6.8 55.5 9.1 1.7 3.3 8.05.6 Comparative data for 212UM01817 + VTU, a Hybrid having II15 as OneInbred Parent Overall comparisons: Year 4 field trials, 8 reps207UM01824 + 603 HC50RR2-1/HCL422 176.6 16.1 11.0 57.1 7.9 0.1 5.5212UM01817 + VTU T0958RMQKZ/II15 196.3 16.3 12.0 56.5 5.8 2.3 5.6207UM01825 + YGC HC50BT1CCR1/HCL422 183.5 16.9 10.9 57.0 3.8 0.1 6.6209UM00819 + 603 HCL116RR2/HCL419 182.2 17.6 10.4 57.8 3.8 1.0 6.3212UM01651 + 603 R2999RHTTB/A3974Z 198.0 18.1 11.0 56.9 1.4 1.8 5.8212UC00824 + VTU R2999RMQKB/A3974Z 198.4 18.1 10.9 56.7 0.4 0.1 5.5206UM01239 + 603 HCL107RR2/HCL506 201.7 18.6 10.9 56.8 4.2 0.1 6.6208UC01435 + YGC HCL116CCR1/HCL419BT1-2 192.2 18.7 10.3 57.0 3.5 2.0 6.0Comparative data for 212UM01817 + VTU and 213UM02792 + N34, HybridsHaving II15 as One Inbred Parent Overall comparisons: Year 5 fieldtrials, 8 reps 212UM01741 + N34 HC50RR2-2/F3121ZNYKZ 157.3 16.6 9.5 53.50.0 0.5 3.4 8.8 5.0 212UM01817 + VTU T0958RMQKZ/II15 176.7 18.9 9.3 53.30.9 2.4 3.3 8.7 6.0 211UA02124 + N34 HCL116RR2/F3632ZNYKZ 170.9 19.3 8.954.4 0.6 1.9 3.6 9.1 5.8 9P8906AM P8906AM 163.1 19.8 8.2 54.7 0.7 0.03.6 8.6 5.5 9DKC38-03RIB DKC38-03RIB 167.9 20.2 8.3 53.4 0.3 0.9 3.3 8.65.7 9DKC39-07RIB DKC39-07RIB 158.8 21.1 7.5 52.2 0.0 1.2 3.2 9.0 6.0213UM02227 + N34 A7202RPGJZ × A4695Z 153.0 21.1 7.2 54.5 0.2 0.0 3.2 8.55.7 213UM02792 + N34 A8075RPGJZ/II15 171.8 22.9 7.5 51.8 0.0 3.3 3.1 8.95.8 212UC00824 + VTU R2999RMQKB/A3974Z 167.3 23.7 7.1 53.1 0.0 0.0 3.48.4 6.0 Comparative data for 212UM01817 + VTU, a Hybrid having II15 asOne Inbred Parent Overall comparisons: Year 5 field trials, 32 reps212UM01741 + N34 HC50RR2-2/F3121ZNYKZ 156.3 16.8 9.3 53.7 1.7 1.6 3.68.7 5.1 212UM01817 + VTU T0958RMQKZ/II15 167.5 18.4 9.1 54.4 3.0 3.6 3.48.9 5.4 211UA02124 + N34 HCL116RR2/F3632ZNYKZ 168.8 19.6 8.6 55.1 2.61.5 3.8 8.7 5.6 9P8906AM P8906AM 163.0 19.8 8.2 55.7 0.6 3.5 4.1 8.8 5.49DKC38-03RIB DKC38-03RIB 172.7 19.8 8.7 54.7 1.1 1.2 3.4 8.7 5.19DKC39-07RIB DKC39-07RIB 168.5 20.5 8.2 53.6 1.1 0.6 3.6 8.9 5.5213UM02227 + N34 A7202RPGJZ × A4695Z 160.7 20.8 7.7 55.5 0.2 0.2 3.5 8.55.4 212UC00824 + VTU R2999RMQKB/A3974Z 170.4 22.4 7.6 54.0 0.4 0.3 3.58.4 5.6 Comparative data for 213UM02792 + N34, a Hybrid having II15 asOne Inbred Parent Overall comparisons: Year 6 field trials, 12 reps213UA02381 + N34 R4168RPGJZ × A2542Z 155.8 18.7 8.3 55.0 0.1 0.0214UA03141 + N34 A6887RPGJZ × HCL4015 156.2 19.7 7.9 53.5 0.3 0.0213UM02227 + N34 A7202RPGJZ × A4695Z 161.0 20.2 8.0 52.7 1.1 0.0214UM02331 + N34 A0036RPGJZ × A1731Z 159.8 20.8 7.7 52.2 0.3 0.2214UA03159 + N34 F5804RPGJZ × R9424Z 154.2 20.8 7.4 55.3 0.4 0.09P8906AM 9P8906AM 148.5 20.8 7.1 53.1 0.1 0.1 9DKC38-03RIB 9DKC38-03RIB159.8 21.3 7.5 51.7 0.1 0.2 213UM02792 + N34 A8075RPGJZ × II15 164.321.4 7.7 50.8 0.3 0.1 214UA03157 + GSS F3030GJLHZ × T9245LFWMZ 166.022.0 7.5 51.2 0.0 0.0 212UC00824 + VTU R2999RMQKB × A3974Z 158.2 22.47.1 51.4 0.0 0.0II17

TABLE 12 Comparative Data for 214UQ01470, a Hybrid Having II17 as OneInbred Parent Overall Comparisons: Year 1 field trials, 8 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 214UQ01470 II17/AB7173.1 14.4 12.0 59.4 4.0 0.0 NA NA 4.2 9DKC43-27 DKC43-27 187.4 15.412.2 58.8 1.9 0.2 NA NA 5.8 209UM00819 + 603 HCL116RR2/HCL419 187.8 15.412.2 59.1 1.0 1.2 NA NA 6.0 209UM00819 + 603 HCL116RR2/HCL419 193.4 15.512.5 58.8 1.0 0.0 NA NA 5.5 9DKC43-27 DKC43-27 182.4 15.7 11.6 58.4 1.10.0 NA NA 5.8 205UQ00792 + NZR HCL107RR2/HCL506BT1 182.9 16.1 11.4 57.90.8 1.0 NA NA 5.7 205UQ00792 + NZR HCL107RR2/HCL506BT1 179.3 16.6 10.858.1 1.6 1.2 NA NA 6.0 Comparative Data for 214UQ01472, a Hybrid HavingII17 as One Inbred Parent Overall Comparisons: Year 2 field trials, 10reps Pedigree Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n214UQ01472 BC110/II17 173.8 15.9 10.9 54.0 2.3 0.0 NA NA 4.2 9DKC52-59DKC52-59 170.6 17.1 10.0 54.0 8.2 0.1 NA NA 5.2 208UC01438 + YGCT2142RBDHZ/HCL519BT1 178.3 17.5 10.2 54.8 3.7 0.5 NA NA 5.2 207UV01124 +YGC HCL301CCR1/HCL516BT1 186.4 18.6 10.0 54.7 10.4 1.1 NA NA 4.49P0463XR P0463 180.5 19.3 9.4 55.1 2.3 7.9 NA NA 5.6 Comparative Datafor 214UQ01474, a Hybrid Having II17 as One Inbred Parent OverallComparisons: Year 3 field trials, 13 reps Pedigree Pedigree YLD Mst %Y/M TWgt SL % RL % EHT PLHT HA n 9DKC43-27 DKC43-27 193.4 16.2 11.9 60.20.0 0.0 40.5 94.8 5.8 209UM00819 + 603 HCL116RR2/HCL419 189.2 16.3 11.661.4 0.3 0.0 48.9 105.5 6.1 9DKC43-27 DKC43-27 191.8 16.6 11.6 59.8 0.00.0 43.3 97.3 6.5 212UC00824 + VTU R2999RMQKB/A3974Z 196.6 16.7 11.860.5 0.0 0.0 44.7 98.7 5.9 210UJ02213 + YGC HCL116CCR1/HCL419BT1 198.916.8 11.8 60.0 0.0 0.4 46.1 101.5 6.1 210UJ02213 + YGCHCL116CCR1/HCL419BT1 205.6 16.9 12.2 60.6 0.0 0.0 45.8 105.5 6.29P9519HR P9519HR 207.3 17.0 12.2 59.4 0.0 3.3 47.5 101.0 6.3212UC00824 + VTU R2999RMQKB/A3974Z 198.5 17.3 11.5 58.4 0.0 0.2 47.295.9 6.1 9P9519HR P9519HR 205.9 17.5 11.8 58.1 0.0 3.2 48.4 103.2 6.2214UQ01474 2107/II17 208.2 18.0 11.6 57.8 0.0 4.2 46.4 98.4 6.5Comparative Data for 214UQ01476 + 017, a Hybrid Having II17 as OneInbred Parent Overall Comparisons: Year 4 field trials, 17 reps PedigreePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 212UA01962 + VTUA8075RMQKZ/R7440Z 193.5 19.0 10.2 56.3 0.0 0.0 41.3 108.3 5.2213UM02223 + VTU R2999RMQKB/HCL4003 197.8 19.9 9.9 55.2 0.2 0.0 43.3110.2 5.7 214UQ01476 + 017 BC110RBDHZ/II17 195.4 20.0 9.8 56.0 0.1 0.039.4 110.5 5.9 9P0062AMX P0062AMX 186.3 20.1 9.3 54.0 0.9 1.4 45.8 108.84.7 9DKC49-94RIB DKC49-94RIB 191.4 20.8 9.2 54.1 0.2 0.0 43.6 104.6 5.7213UR02175 + GSS R2999GJLHZ/R3414LFWMZ 197.8 22.1 9.0 53.6 0.7 0.0 44.3109.0 6.6 Comparative Data for 214UQ03359 + N34, a Hybrid Having II17 asOne Inbred Parent Overall Comparisons: Year 5 field trials GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 214UQ03359 + N34R2999RPGJZ/II17 190.4 19.4 9.8 54.8 0.0 3.4 42.9 106.7 4.7 213UR02175 +GSS R2999GJLHZ/R3414LFWMZ 198.2 21.5 9.2 53.1 0.4 3.5 46.1 116.1 5.39P9917AMX 9P9917AMX 193.2 21.5 9.0 54.6 0.0 4.1 46.1 110.2 4.39DKC49-29RIB 9DKC49-29RIB 190.8 21.8 8.8 53.8 0.0 0.0 45.7 115.4 5.3IM5

TABLE 13 Comparative Data for 211UM02780, a Hybrid Having IM5 as OneInbred Parent Overall comparisons: Year 1 field trials, 6 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % HA n 211UM02780 AB7 × IM5 181.612.8 14.5 56.0 2.1 2.3 5.7 203UK00780 + 603 HC50RR2-1 × SGI901 148.212.9 11.7 57.9 1.9 0.0 5.3 209UM00819 + 603 HCL116RR2 × HCL419 170.813.9 12.6 57.5 0.9 0.3 5.7 206UM01239 + 603 HCL107RR2 × HCL506 187.715.0 13.1 56.3 1.4 1.0 6.3 210UM01241 + 603 HCL112RR2 × HCL437 196.216.0 12.7 56.6 0.7 0.0 6.8 Comparative Data for 211UM02783, a HybridHaving IM5 as One Inbred Parent Overall comparisons: Year 2 fieldtrials, 11 reps Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHTHA n 211UM02783 AB19 × IM5 139.0 19.0 7.6 55.7 5.8 −0.1 3.3 8.3 5.3202UQ01053 + 603 HC37RR2-1 × SGI918 121.7 19.1 6.6 57.1 5.2 0.0 3.1 7.84.8 208UA01447 + YGC HCL116CCR1 × HCL425BT1 128.8 19.6 6.8 57.2 1.2 0.52.8 7.4 4.8 209UM00819 + 603 HCL116RR2 × HCL419 132.2 19.7 6.9 57.2 8.40.3 3.2 8.2 5.1 9DKC35-43 9DKC35-43 123.3 19.8 6.6 56.7 3.1 0.0 3.1 7.85.1 207UM01824 + 603 HC50RR2-1 × HCL422 138.7 20.2 7.3 56.4 4.0 0.5 3.28.1 5.2 208UC01435 + YGC HCL116CCR1 × HCL419BT1-2 136.5 20.7 6.8 56.85.6 0.0 3.4 8.4 5.4 210UM00618 + G11 AB14 × NP2623GTCBLL 138.0 20.9 6.856.3 6.4 0.4 3.2 7.9 4.7 9P8906HR 9P8906HR 134.5 21.1 6.8 56.9 1.8 0.03.4 8.2 5.2 9DKC38-89 9DKC38-89 149.7 21.2 7.4 55.8 0.4 −0.2 3.0 8.3 5.5206UM01239 + 603 HCL107RR2 × HCL506 143.9 22.2 6.8 55.5 8.2 1.3 3.3 8.05.3 Comparative Data for 211UM02783, a Hybrid Having IM5 as One InbredParent Overall comparisons: Year 2 field trials, 17 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 210UK00575 + NZRIV2BT1RR2 × LM3 138.9 16.5 8.6 57.3 3.5 4.6 3.1 7.6 4.8 212UM01721 + N34A9587RHTTZ × A2268ZNYKZ 168.0 17.1 10.1 57.3 1.2 0.4 3.5 8.1 6.39DKC30-20 9DKC30-20 158.7 17.1 9.5 55.9 3.2 1.9 3.7 8.2 5.0 210UA02058 +YGC R5927RBDHZ × A3498ZKDDZ 157.8 17.4 9.4 57.1 3.0 1.6 3.9 8.0 5.1212UM01650 + N34 HCL116RR2 × F7298ZNYKZ 181.5 17.5 10.8 56.4 2.6 0.1 3.48.3 5.3 209UK01912 + YGC R5927RBDHZ × F3745ZKDDZ 152.3 17.5 9.1 57.6 3.80.1 3.3 8.0 5.2 212UK01584 + N34 SGI044RHTTZ × SGI045ZNYKZ 149.8 17.58.8 56.8 1.4 0.2 3.9 8.1 6.6 9P39D97 9P39D97 153.0 17.9 8.9 56.5 1.0 8.63.8 8.0 5.1 211UM02783 AB19 × IM5 177.6 19.5 9.7 54.0 1.9 0.3 3.4 8.26.5 Comparative Data for 212UM01826 + VTU, a Hybrid Having IM5 as OneInbred Parent Overall comparisons: Year 3 field trials, 12 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % HA n 210UK00855 + 603 IV2RR2 × LM3142.2 15.6 9.2 60.6 4.3 11.5 4.4 207UM01824 + 603 HC50RR2-1 × HCL422170.7 15.9 10.8 58.6 1.9 2.2 5.3 213UM00001 + 603 SGI044RHTTZ × SGI045164.9 16.1 10.5 59.4 1.1 0.7 6.1 212UM01650 + N34 HCL116RR2 × F7298ZNYKZ166.8 16.2 10.4 58.6 1.6 1.4 5.2 210UA02058 + YGC R5927RBDHZ ×A3498ZKDDZ 165.8 16.2 10.4 59.3 1.9 0.0 5.3 212UM01826 + VTU IM5 × 2371184.9 16.5 11.5 58.3 7.1 0.0 5.3 207UM01825 + YGC HC50BT1CCR1 × HCL422182.9 16.7 11.1 58.2 0.8 1.8 6.0 209UM00819 + 603 HCL116RR2 × HCL419181.1 17.2 10.7 57.9 3.1 1.1 5.3 212UM01651 + 603 R2999RHTTB × A3974Z204.3 17.3 12.0 56.7 0.3 0.0 5.3 212UC00824 + VTU R2999RMQKB × A3974Z194.4 17.3 11.4 57.7 0.6 0.0 5.4 Comparative Data for 212UM01827 + VTU,a Hybrid Having IM5 as One Inbred Parent Overall comparisons: Year 3field trials, 12 reps Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % HAn 207UM01825 + YGC HC50BT1CCR1 × HCL422 174.2 16.0 11.0 60.8 0.0 0.2 5.5212UM01827 + VTU 2107 × IM5 197.7 16.9 11.8 59.1 0.1 0.0 6.8212UM01723 + VTU A8668RMQKZ × T0813Z 179.3 17.3 10.7 59.3 0.1 2.0 5.5210UR01118 + VTU HCL112CCR1 × A5338ZNYKZ 188.5 17.8 10.9 59.6 0.0 0.06.0 208UC01435 + YGC HCL116CCR1 × HCL419BT1-2 195.4 17.9 11.3 59.1 0.0−0.3 6.1 212UC00824 + VTU R2999RMQKB × A3974Z 188.1 18.0 10.7 59.1 0.00.0 5.7 210UA02079 + YGC R2999RBDHZ × HCL4003BT1 202.1 19.1 10.9 57.90.0 0.2 6.3 Comparative Data for 212UM01826 + VTU and 212UM01827 + VTU,Hybrids Having IM5 as One Inbred Parent Overall comparisons: Year 4field trials, 17 reps Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHTPLHT HA n 212UM01826 + VTU IM5 × 2371 166.0 17.0 9.9 53.9 5.0 0.7 3.48.6 5.9 212UM01741 + N34 HC50RR2-2 × F3121ZNYKZ 163.4 17.3 9.5 54.9 1.01.3 3.3 8.6 5.1 9P8906AM 9P8906AM 168.9 19.5 8.7 55.6 1.1 1.0 3.9 9.15.7 9DKC38-03RIB 9DKC38-03RIB 174.9 19.8 9.0 54.4 2.1 0.6 3.5 8.7 5.5212UM01723 + VTU A8668RMQKZ × T0813Z 168.3 19.8 8.6 54.0 0.7 1.3 3.5 8.95.6 9DKC39-07RIB 9DKC39-07RIB 171.9 20.2 8.6 53.6 0.7 1.0 3.5 9.1 5.8212UM01827 + VTU 2107 × IM5 170.2 20.7 8.5 53.2 4.3 0.8 3.4 8.7 6.3213UM02227 + N34 A7202RPGJZ × A4695Z 160.5 20.7 7.9 55.5 0.3 0.2 3.2 8.75.2 212UC00824 + VTU R2999RMQKB × A3974Z 174.1 22.5 7.9 53.8 3.6 −0.13.5 8.5 5.6 Comparative Data for 212UM01822, a Hybrid Having IM5 as OneInbred Parent Overall comparisons: Year 4 field trials, 18 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 212UM01741 + N34HC50RR2-2 × F3121ZNYKZ 165.0 16.8 9.8 54.9 2.3 1.5 3.5 8.9 5.1212UM01822 AB14 × IM5 172.4 18.3 9.5 55.6 2.7 0.7 3.1 8.4 5.1212UM01723 + VTU A8668RMQKZ × T0813Z 170.6 18.9 9.1 54.5 1.4 0.5 3.5 8.95.8 9DKC38-03RIB 9DKC38-03RIB 173.4 19.2 9.2 54.7 0.9 0.7 3.7 8.9 5.2213UM02227 + N34 A7202RPGJZ × A4695Z 166.0 20.8 8.1 55.8 0.7 0.0 3.6 8.95.5 212UC00824 + VTU R2999RMQKB × A3974Z 168.2 22.0 7.7 54.1 0.3 0.2 3.58.6 5.4 Comparative Data for 212UM01826 + VTU, a Hybrid Having IM5 asOne Inbred Parent Overall comparisons: Year 4 field trials, 16 repsGenotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n212UM01741 + N34 HC50RR2-2 × F3121ZNYKZ 156.3 16.8 9.3 53.7 1.7 1.6 3.68.7 5.1 212UM01826 + VTU IM5 × 2371 166.8 17.6 9.7 53.9 2.5 2.7 3.4 8.65.8 212UM01723 + VTU A8668RMQKZ × T0813Z 165.6 19.2 8.8 54.3 0.8 1.7 3.58.7 5.3 9DKC38-03RIB 9DKC38-03RIB 172.7 19.8 8.9 54.7 1.1 1.2 3.4 8.75.1 9P8906AM 9P8906AM 163.0 19.8 8.3 55.7 0.6 3.5 4.1 8.8 5.49DKC39-07RIB 9DKC39-07RIB 168.5 20.5 8.4 53.6 1.1 0.6 3.6 8.9 5.5213UM02227 + N34 A7202RPGJZ × A4695Z 160.7 20.8 7.8 55.5 0.2 0.2 3.5 8.55.4 212UC00824 + VTU R2999RMQKB × A3974Z 170.4 22.4 7.8 54.0 0.4 0.3 3.58.4 5.6LK1

TABLE 14 Comparative Data for 213US00930 + VTU, a Hybrid Having LK1 asOne Inbred Parent Overall comparisons: Year 1 field trials, 12 repsGenotype Pedigree YLD Mst % Y/M TWgt SL % RL % HA n 212UA01960 + VTUA7196RMQKZ × T6053Z 166.4 19.7 8.4 55.9 1.9 0.0 5.5 211UV00415 + 017BB46 × MM27RMQKZ 172.3 19.8 8.7 53.7 0.0 0.0 5.8 209UC00596 + VTU BB59 ×A1555RMQKZ 169.6 19.8 8.6 53.2 0.0 0.0 6.4 213US00930 + VTU A7196RMQKZ ×LK1 181.2 20.5 8.8 53.2 3.6 0.0 5.2 209UC00595 + VTU BB38 × A1555RMQKZ166.6 20.8 8.0 52.2 0.0 0.0 6.7 211UU01785 + VTU CB15 × A1555RMQKZ 177.321.9 8.1 52.3 0.1 2.4 6.7 9DKC66-96 9DKC66-96 173.5 22.4 7.7 53.9 0.80.4 6.1 Comparative Data for 213UC01710 + 603, a Hybrid Having LK1 asOne Inbred Parent Overall comparisons: Year 2 field trials, 6 repsGenotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n213UC01710 + 603 CC8RHTTZ × LK1 204.2 20.1 10.2 54.5 1.4 0.0 3.8 10.05.5 9P1498HR 9P1498HR 212.2 21.0 10.1 55.0 0.0 0.0 4.3 10.3 5.39DKC66-96 9DKC66-96 209.9 21.5 9.8 54.6 0.0 0.0 2.8 9.4 5.5 ComparativeData for 213UC01711 + 603, a Hybrid Having LK1 as One Inbred ParentOverall comparisons: Year 2 field trials, 11 reps Genotype Pedigree YLDMst % Y/M TWgt SL % RL % EHT PLHT HA n 213UC01711 + 603 CB13RHTTZ × LK1199.3 19.9 10.0 55.2 0.0 0.0 3.9 11.0 5.5 212UJ00007 + VTU BB87 ×A1555RMQKZ 200.8 20.1 10.0 53.7 0.6 0.0 3.6 10.7 5.7 9P1498HR 9P1498HR209.0 20.4 10.2 56.0 0.4 0.0 3.7 10.5 5.6 210UJ02224 + N34 BB36RR2 ×A1555ZNYKZ 194.3 20.8 9.3 54.2 0.1 0.0 3.6 10.2 5.3 211UU01785 + VTUCB15 × A1555RMQKZ 194.8 20.9 9.3 54.1 0.0 0.0 3.4 10.7 5.0 9DKC66-969DKC66-96 199.6 21.6 9.2 54.9 0.0 0.0 3.9 10.3 5.5 207UC01707 + YGC CC1× LH287BT1CCR1 193.1 22.1 8.7 53.4 1.0 0.0 3.0 10.3 5.5 Comparative Datafor 213US01135 + VTU, a Hybrid Having LK1 as One Inbred Parent Overallcomparisons: Year 2 field trials, 23 reps Genotype Pedigree YLD Mst %Y/M TWgt SL % RL % EHT PLHT HA n 213US01135 + VTU BB36RMQKZ × LK1 220.020.6 10.7 54.0 1.7 0.0 3.7 10.3 6.3 212UJ00007 + VTU BB87 × A1555RMQKZ218.4 21.5 10.2 52.8 2.6 1.1 3.8 9.9 6.2 9DKC62-97RIB 9DKC62-97RIB 217.321.5 10.1 55.3 1.2 0.0 3.5 9.4 6.4 209UC00595 + VTU BB38 × A1555RMQKZ220.9 21.6 10.2 53.7 0.5 0.2 3.4 9.8 6.2 9P1339AM1 9P1339AM1 214.5 22.89.4 55.2 1.5 0.1 3.8 10.1 6.3 211UU01785 + VTU CB15 × A1555RMQKZ 216.323.5 9.2 52.9 1.6 0.3 3.4 9.9 6.8 213UU02222 + GSS A7196GJLHZ ×T6053LFWMZ 230.9 23.6 9.8 54.5 0.2 0.0 4.0 9.1 6.8 Comparative Data for214US01543 + VTU, a Hybrid Having LK1 as One Inbred Parent Overallcomparisons: Year 3 field trials, 23 reps Genotype Pedigree YLD Mst %Y/M TWgt SL % RL % EHT PLHT HA n 214US01543 + VTU MEF2526RMQKZ × LK1205.2 20.3 10.1 53.9 0.6 0.3 4.0 9.8 5.8 211US00896 + 017 BB38RMQKZ ×MM53 198.2 20.5 9.7 53.1 1.0 10.6 3.9 9.7 5.4 209UC00596 + VTU BB59 ×A1555RMQKZ 202.6 21.2 9.6 52.4 0.0 1.7 3.6 9.6 5.7 9DKC57-75RIB9DKC57-75RIB 199.2 21.3 9.4 53.9 1.3 1.7 3.6 9.1 5.5 213UA02359 + GSSA3027RMQKZ × A2959LMSLZ 206.1 21.7 9.5 53.7 0.5 2.0 4.0 9.5 5.69P0832AMX 9P0832AMX 202.4 22.8 8.9 54.6 0.1 16.4 3.8 9.8 5.6 ComparativeData for 214UV02540, 214UV02541, and 214UV02542, Hybrids Having LK1 asOne Inbred Parent Overall comparisons: Year 3 field trials, 24 repsGenotype Pedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 214UV02540BB85 × LK1 214.4 17.9 12.0 53.8 7.3 0.5 3.7 10.5 4.7 214UV02541 CB20 ×LK1 217.6 19.1 11.4 54.0 4.6 0.0 4.3 10.2 5.4 214UV02542 CB21 × LK1210.5 19.1 11.0 54.2 2.3 0.2 3.8 10.2 5.4 9DKC63-33RIB 9DKC63-33RIB223.5 19.4 11.5 56.1 1.3 0.2 3.9 9.7 5.3 9P1221AMXT 9P1221AMXT 203.419.4 10.5 56.8 4.8 1.4 4.1 10.0 5.0 209UC00595 + VTU BB38 × A1555RMQKZ223.2 20.9 10.7 53.5 1.2 0.2 3.7 9.9 5.9 212UJ00007 + VTU BB87 ×A1555RMQKZ 224.7 21.2 10.6 53.6 3.3 2.9 3.8 9.9 6.0 213UU02222 + GSSA7196GJLHZ × T6053LFWMZ 234.9 22.8 10.3 55.3 0.1 0.0 3.6 9.4 6.6211UU01785 + VTU CB15 × A1555RMQKZ 225.1 22.9 9.8 53.5 1.8 0.7 3.1 9.66.0 Comparative Data for 213US01135 + VTU, a Hybrid Having LK1 as OneInbred Parent Overall comparisons: Year 3 field trials, 64 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % EHT PLHT HA n 213US01135 + VTUBB36RMQKZ × LK1 217.1 19.1 11.4 54.2 2.1 0.8 3.5 9.8 5.7 9DKC63-33RIB9DKC63-33RIB 220.8 19.7 11.2 55.7 1.7 1.6 3.4 9.0 5.4 209UC00595 + VTUBB38 × A1555RMQKZ 215.7 20.8 10.4 53.8 0.5 0.7 3.1 9.2 6.0 9P1221AMXT9P1221AMXT 207.7 20.9 9.9 56.2 0.6 0.4 3.8 9.6 5.5 212UJ00007 + VTU BB87× A1555RMQKZ 222.4 21.1 10.5 53.1 2.2 2.0 3.3 9.3 6.1 213UC01433 + GSSBB36GJLHZ × A1555LFWMZ 217.1 22.1 9.8 52.8 1.3 1.3 3.3 9.2 6.1211UU01785 + VTU CB15 × A1555RMQKZ 223.7 22.6 9.9 53.4 2.0 2.7 2.9 9.26.1 213UU02222 + GSS A7196GJLHZ × T6053LFWMZ 230.6 22.8 10.1 55.1 0.40.5 3.3 8.6 6.4 Comparative Data for 213US01135 + VTU, a Hybrid HavingLK1 as One Inbred Parent Overall comparisons: Year 3 field trials, 85reps Genotype Pedigree YLD Mst % Y/M TWgt SL % RL % 213US01135 + VTUBB36RMQKZ × LK1 223.0 17.1 13.0 56.4 2.3 0.2 9P1221AMXT 9P1221AMXT 219.417.9 12.3 59.1 2.2 0.5 213UA02390 + GSS T3997GJLHZ × T6053LFWMZ 231.118.5 12.5 57.1 0.4 0.1 209UC00595 + VTU BB38 × A1555RMQKZ 228.9 18.612.3 55.6 0.8 0.1 212UJ00007 + VTU BB87 × A1555RMQKZ 228.0 18.8 12.154.4 0.5 0.4 213UC01433 + GSS BB36GJLHZ × A1555LFWMZ 220.0 18.9 11.654.7 0.9 0.5 213UU02222 + GSS A7196GJLHZ × T6053LFWMZ 239.5 19.0 12.658.4 0.5 0.2 211UU01785 + VTU CB15 × A1555RMQKZ 231.6 19.3 12.0 55.7 0.70.2MM65

TABLE 15 Comparative Data for 212UU02866 + VTU, a Hybrid Having MM65 asOne Inbred Parent Overall comparisons: 64 reps Genotype Pedigree YLD Mst% Y/M TWgt SL % RL % EHT PLHT HA n 9DKC63-33RIB 9DKC63-33RIB 220.9 19.811.2 55.7 1.7 1.6 3.4 9.0 5.4 209UC00595 + VTU BB38 × 7610 215.7 20.810.4 53.8 0.5 0.7 3.1 9.2 6.0 9P1221AMXT 9P1221AMXT 207.7 20.9 9.9 56.20.6 0.4 3.8 9.6 5.6 212UJ00007 + VTU BB87 × 7610 222.4 21.1 10.5 53.12.2 2.0 3.3 9.3 6.1 212UU02866 + VTU BB36 × MM65RMQKZ 217.2 21.1 10.354.3 1.3 3.1 3.3 9.4 5.8 213UA02371 + VTU 2361 × 8084 229.3 21.3 10.855.1 0.3 1.1 3.6 9.0 6.1 213UC01433 + GSS BB36GJLHZ × 7641 217.1 22.19.8 52.8 1.3 1.3 3.3 9.2 6.1 213UA02677 + GSS 2220 × 8030 219.2 22.2 9.955.7 0.4 1.1 3.4 9.5 6.1 211UU01785 + VTU CB15 × 7610 223.7 22.6 9.953.4 2.0 2.7 2.9 9.2 6.1 213UU02222 + GSS 2195 × 8026 230.6 22.9 10.155.1 0.4 0.5 3.3 8.6 6.4 Comparative Data for 212UU02866 + VTU, a HybridHaving MM65 as One Inbred Parent Overall comparisons: 18 reps GenotypePedigree YLD Mst % Y/M TWgt SL % RL % HA n 213UU01991 + VTU BB86RMQKZ ×MM69 208.3 22.1 9.4 52.9 0.0 0.0 5.7 213US01135 + VTU BB36RMQKZ × LK1217.7 22.4 9.7 52.7 0.0 0.0 6.0 211UC01200 + VTU 2033 × ML12 222.0 23.19.6 54.1 0.0 0.0 5.6 213UC00848 + VTU BB210 × 7610 220.7 23.5 9.4 52.60.0 0.0 5.8 213UC00864 + VTU CB34 × 7610 232.6 23.7 9.8 52.5 0.0 0.0 5.9213UC01452 + VTU BB209 × 7610 220.5 23.7 9.3 52.8 0.0 −0.1 5.6209UC00595 + VTU BB38 × 7610 224.9 24.0 9.4 53.0 0.0 0.0 5.9213UE00994 + VTU BC146 × 7610 217.6 24.0 9.1 52.9 0.0 0.1 6.0213UU03346 + N34 2361 × 8084 213.9 24.0 8.9 53.9 0.1 0.1 5.8212UU02866 + VTU BB36 × MM65RMQKZ 225.1 24.1 9.3 53.2 0.0 3.7 5.5213UC01433 + GSS BB36GJLHZ × 7641 218.2 24.7 8.8 52.5 0.0 0.0 6.1211UU01785 + VTU CB15 × 7610 227.6 24.9 9.1 52.7 0.0 0.0 6.1212UJ00007 + VTU BB87 × 7610 225.2 24.9 9.0 52.7 0.0 0.0 6.1213UE01007 + VTU BC147 × 7610 226.1 25.0 9.0 52.5 0.0 0.1 6.2210UJ02224 + N34 BB36RR2 × 7632 208.2 25.1 8.3 53.0 0.0 0.0 5.9214UA03131 + N34 2196 × 7729 222.4 26.1 8.5 53.6 0.6 0.7 5.8213UU02222 + GSS 2195 × 8026 226.4 27.0 8.4 53.7 0.1 0.0 6.5

The foregoing detailed description has been given for clearness ofunderstanding only and no unnecessary limitations should be understoodthere from as modifications will be obvious to those skilled in the art.

While a number of exemplary aspects and embodiments have been discussedabove, those of skill in the art will recognize certain modifications,permutations, additions and sub-combinations thereof. It is thereforeintended that the following appended claims and claims hereafterintroduced are interpreted to include all such modifications,permutations, additions and sub-combinations as are within their truespirit and scope.

Unless defined otherwise, all technical and scientific terms herein havethe same meaning as commonly understood by one of ordinary skill in theart to which this invention belongs. Although any methods and materials,similar or equivalent to those described herein, can be used in thepractice or testing of the present invention, the preferred methods andmaterials are described herein. All publications, patents, and patentpublications cited are incorporated by reference herein in theirentirety for all purposes.

The publications discussed herein are provided solely for theirdisclosure prior to the filing date of the present application. Nothingherein is to be construed as an admission that the present invention isnot entitled to antedate such publication by virtue of prior invention.

While the invention has been described in connection with specificembodiments thereof, it will be understood that it is capable of furthermodifications and this application is intended to cover any variations,uses, or adaptations of the invention following, in general, theprinciples of the invention and including such departures from thepresent disclosure as come within known or customary practice within theart to which the invention pertains and as may be applied to theessential features hereinbefore set forth and as follows in the scope ofthe appended claims.

What is claimed is:
 1. A seed of inbred corn line designated II15,wherein a representative sample of seed of said line was deposited underATCC Accession No. PTA-123784.
 2. A corn plant, or a part thereof,produced by growing the seed of claim
 1. 3. A corn plant, or a partthereof, having all the physiological and morphological characteristicsof inbred line II15, wherein a representative sample of seed of saidline was deposited under ATCC Accession No. PTA-123784.
 4. A tissueculture of cells produced from the plant of claim
 2. 5. A corn plantregenerated from the tissue culture of claim 4, wherein the regeneratedplant has all the morphological and physiological characteristics ofinbred line II15, wherein a representative sample of seed of said linewas deposited under ATCC Accession No. PTA-123784.
 6. A method forproducing a hybrid corn seed wherein the method comprises crossing theplant of claim 2 with a different corn plant and harvesting theresultant hybrid corn seed.
 7. A hybrid corn seed produced by the methodof claim
 6. 8. A hybrid corn plant produced by growing the seed of claim7.
 9. A method for producing inbred corn line II15, wherein arepresentative sample of seed of said line was deposited under ATCCAccession No. PTA-123784, wherein the method comprises: a) planting acollection of seeds comprising seed of a hybrid, one of whose parents isinbred line II15, said collection also comprising seed of said inbred;b) growing plants from said collection of seeds; c) identifying theplants having the physiological and morphological characteristics ofinbred corn line II15 as inbred parent plants; d) controllingpollination of said inbred parent plants in a manner which preserves thehomozygosity of said inbred parent plant; and e) harvesting theresultant seed and thereby producing an inbred corn line having all ofthe physiological and morphological characteristics of inbred corn lineII15.
 10. The method of claim 9 wherein step (c) comprises identifyingplants with decreased vigor compared to the other plants grown from thecollection of seeds.
 11. A method for producing a corn plant thatcontains in its genetic material one or more transgenes, wherein themethod comprises crossing the corn plant of claim 2 with either a secondplant of another corn line which contains a transgene or a transformedcorn plant of the inbred corn line II15, so that the genetic material ofthe progeny plant that results from the cross contains the transgene(s)operably linked to a regulatory element and wherein the transgene isselected from the group consisting of male sterility, male fertility,herbicide resistance, insect resistance, disease resistance, waterstress tolerance, and increased digestibility.
 12. A corn plant producedby the method of claim
 11. 13. The corn plant of claim 12, wherein thetransgene confers resistance to an herbicide selected from the groupconsisting of imidazolinone, sulfonylurea, glyphosate, glufosinate,L-phosphinothricin, triazine and benzonitrile.
 14. The corn plant ofclaim 12, wherein the transgene encodes a Bacillus thuringiensisprotein.
 15. The corn plant of claim 12, wherein the transgene confersdisease resistance.
 16. The corn plant of claim 12, wherein thetransgene confers water stress tolerance.
 17. The corn plant of claim12, wherein the transgene confers increased digestibility.
 18. A methodfor producing a hybrid corn seed wherein the method comprises crossingthe plant of claim 12 with a different corn plant and harvesting theresultant hybrid corn seed.
 19. A method of producing a corn plant withincreased waxy starch or increased amylose starch wherein the methodcomprises transforming the corn plant of claim 2 with a transgene thatmodifies waxy starch or amylose starch metabolism, thereby producing acorn plant with increased waxy starch or amylose starch metabolism. 20.A corn plant produced by the method of claim
 19. 21. A method ofintroducing one or more desired traits into inbred corn line II15wherein the method comprises: a) crossing the inbred line II15 plantsgrown from the inbred line II15 seed, wherein a representative sample ofseed of said line was deposited under ATCC Accession No. PTA-123784,with plants of another corn line that comprise one or more desiredtraits to produce progeny plants, wherein the one or more desired traitsare selected from the group consisting of male sterility, malefertility, herbicide resistance, insect resistance, disease resistance,waxy starch, water stress tolerance, increased amylose starch andincreased digestibility; b) selecting progeny plants that have the oneor more desired traits to produce selected progeny plants; c) crossingthe selected progeny plants with the inbred corn line II15 plants toproduce backcross progeny plants; d) selecting for backcross progenyplants that have the one or more desired traits and physiological andmorphological characteristics of inbred corn line II15 listed in Table1J to produce selected backcross progeny plants; and e) repeating steps(c) and (d) one or more times in succession to produce selected secondor higher backcross progeny plants that comprise the desired one or moretraits and all of the physiological and morphological characteristics ofinbred corn line II15 as listed in Table 1J.
 22. A corn plant producedby the method of claim 21, wherein the plant has the one or more desiredtraits and otherwise all of the physiological and morphologicalcharacteristics of inbred corn line II15, wherein a representativesample of seed of said line was deposited under ATCC Accession No.PTA-123784.
 23. A method for producing inbred corn line II15 seed,wherein a representative sample of seed of said line was deposited underATCC Accession No. PTA-123784, wherein the method comprises crossing afirst inbred parent corn plant with a second inbred parent corn plantand harvesting the resultant corn seed, wherein both said first andsecond inbred corn plant are the corn plant of claim
 2. 24. A method forproducing inbred corn line II15 seed, wherein a representative sample ofseed of said line was deposited under ATCC Accession No. PTA-123784,wherein the method comprises: a) planting the inbred corn seed of claim1; b) growing a plant from said seed; c) controlling pollination in amanner that the pollen produced by the grown plant pollinates the ovulesproduced by the grown plant; and d) harvesting the resultant seed andthereby producing an inbred corn line having all of the physiologicaland morphological characteristics of inbred corn line II15.
 25. A methodfor producing a corn seed that contains in its genetic material one ormore transgenes, wherein the method comprises crossing the corn plant ofclaim 2 with either a second plant of another corn line which containsone or more transgenes or a transformed corn plant of the inbred cornline II15, wherein the transgene(s) is operably linked to a regulatoryelement and wherein the transgene is selected from the group consistingof male sterility, male fertility, herbicide resistance, insectresistance, disease resistance, water stress tolerance, and increaseddigestibility; and harvesting the resultant seed.
 26. A corn seed, or apart thereof, produced by the method of claim
 25. 27. A method forproducing a hybrid corn seed wherein the method comprises crossing theplant of claim 22 with a different corn plant and harvesting theresultant hybrid corn seed.
 28. A hybrid corn seed produced by themethod of claim
 27. 29. A method of producing a corn product, saidmethod comprising the step of milling the inbred seed of claim 1,thereby producing the corn product.
 30. The method of claim 29, whereinthe corn product is selected from the group consisting of corn meal,corn flour, corn starch, corn syrup, corn sweetener and corn oil.
 31. Amethod of producing a corn product, said method comprising the step ofmilling the hybrid seed of claim 7, thereby producing the corn product.32. The method of claim 31, wherein the corn product is selected fromthe group consisting of corn meal, corn flour, corn starch, corn syrup,corn sweetener and corn oil.